Determination of Nonderivatized para-Hydroxylated Metabolites of Diazepam in Biological Fluids with a GC Megabore Column System

Determination of Nonderivatized para-Hydroxylated Metabolites of Diazepam in Biological Fluids with a GC Megabore Column System

A method was developed using gas chromatography (GC) and a Megabore column system capable of simultaneous detection of diazapam, N-desmethyldiazepam, temazepam, oxazepam, and their para-hydroxylated metabolites. This method does not require derivatization of para-hydroxylated metabolites. Standard c...

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Veröffentlicht in:Journal of analytical toxicology 1992-07, Vol.16 (4), p.236-239
Hauptverfasser: Beischlag, T.V., Inaba, T.
Format: Artikel
Sprache:eng
Schlagworte:
Analysis
Animals
Biological and medical sciences
Chromatography, Gas
Diazepam - metabolism
Diazepam - urine
General pharmacology
Hydroxylation
Male
Medical sciences
Nordazepam - urine
Oxazepam - urine
Pharmacology. Drug treatments
Rats
Rats, Inbred Strains
Temazepam - urine
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Beschreibung
Zusammenfassung:A method was developed using gas chromatography (GC) and a Megabore column system capable of simultaneous detection of diazapam, N-desmethyldiazepam, temazepam, oxazepam, and their para-hydroxylated metabolites. This method does not require derivatization of para-hydroxylated metabolites. Standard curves for pure reference compounds were linear, with the minimum detectable concentration of diazepam and its metabolites as low as 0.13 ng/injection.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/16.4.236