In vitro assay for the biosynthesis and metabolism of juvenile hormone by exposed corpora allata of Aedes aegypti (Diptera: Culicidae)
A technique has been developed to study JH III biosynthesis in vitro by removing the head and thorax of Aedes aegypti (L.) and exposing the corpora allata (CA). Exposed CA were incubated with [12-3H] methyl farnesoate, and the newly synthesized JH III and JH III metabolites were followed using C18 r...
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| Veröffentlicht in: | Journal of medical entomology 1992-03, Vol.29 (2), p.318-324 |
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| creator | Borovsky, D. (IFAS-University of Florida, Vero Beach, FL) Carlson, D.A |
| description | A technique has been developed to study JH III biosynthesis in vitro by removing the head and thorax of Aedes aegypti (L.) and exposing the corpora allata (CA). Exposed CA were incubated with [12-3H] methyl farnesoate, and the newly synthesized JH III and JH III metabolites were followed using C18 reversed-phase, high-performance liquid chromatography (HPLC) and preparative gas chromatography. The rate of synthesis of [12-3H] JH III by exposed CA from newly emerged adult Ae. aegypti was 23 fmol/CA/h. The rate of synthesis of [12-3H] JH III decreased 4-fold 3 d after adult eclosion and increased to 23 fmol/CA/h 5 h after the blood meal. Exposed CA of blood-fed and sugar-fed Ae. aegypti were also incubated with L-[methyl-3H] methionine, and the rate of synthesis of JH III was studied. The rate of JH III biosynthesis increased immediately after the blood meal as was found with [12-3H] methyl farnesoate. The potential application of methyl farnesoate in monitoring the de novo synthesis of JH III in mosquitoes in vitro and in vivo is discussed |
| doi_str_mv | 10.1093/jmedent/29.2.318 |
| format | Article |
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(IFAS-University of Florida, Vero Beach, FL) ; Carlson, D.A</creator><creatorcontrib>Borovsky, D. (IFAS-University of Florida, Vero Beach, FL) ; Carlson, D.A</creatorcontrib><description>A technique has been developed to study JH III biosynthesis in vitro by removing the head and thorax of Aedes aegypti (L.) and exposing the corpora allata (CA). Exposed CA were incubated with [12-3H] methyl farnesoate, and the newly synthesized JH III and JH III metabolites were followed using C18 reversed-phase, high-performance liquid chromatography (HPLC) and preparative gas chromatography. The rate of synthesis of [12-3H] JH III by exposed CA from newly emerged adult Ae. aegypti was 23 fmol/CA/h. The rate of synthesis of [12-3H] JH III decreased 4-fold 3 d after adult eclosion and increased to 23 fmol/CA/h 5 h after the blood meal. Exposed CA of blood-fed and sugar-fed Ae. aegypti were also incubated with L-[methyl-3H] methionine, and the rate of synthesis of JH III was studied. The rate of JH III biosynthesis increased immediately after the blood meal as was found with [12-3H] methyl farnesoate. The potential application of methyl farnesoate in monitoring the de novo synthesis of JH III in mosquitoes in vitro and in vivo is discussed</description><identifier>ISSN: 0022-2585</identifier><identifier>EISSN: 1938-2928</identifier><identifier>DOI: 10.1093/jmedent/29.2.318</identifier><identifier>PMID: 1495050</identifier><identifier>CODEN: JMENA6</identifier><language>eng</language><publisher>Lanham, MD: Entomological Society of America</publisher><subject>Aedes - metabolism ; AEDES AEGYPTI ; Animals ; Biochemistry. Physiology. Immunology ; Biological and medical sciences ; BIOSINTESIS ; BIOSYNTHESE ; Chromatography, Gas ; Chromatography, High Pressure Liquid ; Corpora Allata - metabolism ; Culicidae ; Diptera ; Female ; Fundamental and applied biological sciences. Psychology ; HORMONAS JUVENILES ; HORMONE JUVENILE ; Insecta ; Invertebrates ; Juvenile Hormones - biosynthesis ; Juvenile Hormones - metabolism ; METABOLISME ; METABOLISMO ; Methionine - metabolism ; Physiology. Development ; SECRECION ; SECRETION ; SISTEMA NERVIOSO ; SYSTEME NERVEUX</subject><ispartof>Journal of medical entomology, 1992-03, Vol.29 (2), p.318-324</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c288t-4fc8ca330b361b2e1c346c8c958838a8ea23bcad9705a68d9c1d9a0dd9d12f33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5195792$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1495050$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Borovsky, D. (IFAS-University of Florida, Vero Beach, FL)</creatorcontrib><creatorcontrib>Carlson, D.A</creatorcontrib><title>In vitro assay for the biosynthesis and metabolism of juvenile hormone by exposed corpora allata of Aedes aegypti (Diptera: Culicidae)</title><title>Journal of medical entomology</title><addtitle>J Med Entomol</addtitle><description>A technique has been developed to study JH III biosynthesis in vitro by removing the head and thorax of Aedes aegypti (L.) and exposing the corpora allata (CA). Exposed CA were incubated with [12-3H] methyl farnesoate, and the newly synthesized JH III and JH III metabolites were followed using C18 reversed-phase, high-performance liquid chromatography (HPLC) and preparative gas chromatography. The rate of synthesis of [12-3H] JH III by exposed CA from newly emerged adult Ae. aegypti was 23 fmol/CA/h. The rate of synthesis of [12-3H] JH III decreased 4-fold 3 d after adult eclosion and increased to 23 fmol/CA/h 5 h after the blood meal. Exposed CA of blood-fed and sugar-fed Ae. aegypti were also incubated with L-[methyl-3H] methionine, and the rate of synthesis of JH III was studied. The rate of JH III biosynthesis increased immediately after the blood meal as was found with [12-3H] methyl farnesoate. The potential application of methyl farnesoate in monitoring the de novo synthesis of JH III in mosquitoes in vitro and in vivo is discussed</description><subject>Aedes - metabolism</subject><subject>AEDES AEGYPTI</subject><subject>Animals</subject><subject>Biochemistry. Physiology. Immunology</subject><subject>Biological and medical sciences</subject><subject>BIOSINTESIS</subject><subject>BIOSYNTHESE</subject><subject>Chromatography, Gas</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Corpora Allata - metabolism</subject><subject>Culicidae</subject><subject>Diptera</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HORMONAS JUVENILES</subject><subject>HORMONE JUVENILE</subject><subject>Insecta</subject><subject>Invertebrates</subject><subject>Juvenile Hormones - biosynthesis</subject><subject>Juvenile Hormones - metabolism</subject><subject>METABOLISME</subject><subject>METABOLISMO</subject><subject>Methionine - metabolism</subject><subject>Physiology. Development</subject><subject>SECRECION</subject><subject>SECRETION</subject><subject>SISTEMA NERVIOSO</subject><subject>SYSTEME NERVEUX</subject><issn>0022-2585</issn><issn>1938-2928</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtr3DAUhUVpSSdJ96VQ0KKEduGJHqMZqbswfSQQyKLpWlxL14kG23IlO9R_oL87GmZol91cXXS-ezhwCHnL2ZIzIy93HXrsx0thlmIpuX5BFtxIXQkj9EuyYEyISiitXpPTnHeMMc1X5oSclKmYYgvy56anT2FMkULOMNMmJjo-Iq1DzHNfthwyhd7TDkeoYxtyR2NDd9MT9qFF-hhTF_vCzxR_DzGjpy6mISag0LYwwp6-KiGLCz7Mwxjoxy9hGDHBZ7qd2uCCB_x0Tl410GZ8c3zPyP23r_fb6-r27vvN9uq2ckLrsVo1TjuQktVyzWuB3MnVunwZpbXUoBGErB14s2EK1tobx70B5r3xXDRSnpGLg-2Q4q8J82i7kB2WoD3GKduN5FwqLf4L8jUXRsm9IzuALsWcEzZ2SKGDNFvO7L4ie6zICmOFLRWVk_dH76ku2r-DQydF_3DUITtomwS9C_kvprhRG7OP-O6ANRAtPKSC_PxhuF5tmJDPdfqlNQ</recordid><startdate>199203</startdate><enddate>199203</enddate><creator>Borovsky, D. (IFAS-University of Florida, Vero Beach, FL)</creator><creator>Carlson, D.A</creator><general>Entomological Society of America</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>199203</creationdate><title>In vitro assay for the biosynthesis and metabolism of juvenile hormone by exposed corpora allata of Aedes aegypti (Diptera: Culicidae)</title><author>Borovsky, D. (IFAS-University of Florida, Vero Beach, FL) ; Carlson, D.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c288t-4fc8ca330b361b2e1c346c8c958838a8ea23bcad9705a68d9c1d9a0dd9d12f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Aedes - metabolism</topic><topic>AEDES AEGYPTI</topic><topic>Animals</topic><topic>Biochemistry. Physiology. Immunology</topic><topic>Biological and medical sciences</topic><topic>BIOSINTESIS</topic><topic>BIOSYNTHESE</topic><topic>Chromatography, Gas</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Corpora Allata - metabolism</topic><topic>Culicidae</topic><topic>Diptera</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HORMONAS JUVENILES</topic><topic>HORMONE JUVENILE</topic><topic>Insecta</topic><topic>Invertebrates</topic><topic>Juvenile Hormones - biosynthesis</topic><topic>Juvenile Hormones - metabolism</topic><topic>METABOLISME</topic><topic>METABOLISMO</topic><topic>Methionine - metabolism</topic><topic>Physiology. Development</topic><topic>SECRECION</topic><topic>SECRETION</topic><topic>SISTEMA NERVIOSO</topic><topic>SYSTEME NERVEUX</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Borovsky, D. (IFAS-University of Florida, Vero Beach, FL)</creatorcontrib><creatorcontrib>Carlson, D.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical entomology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Borovsky, D. (IFAS-University of Florida, Vero Beach, FL)</au><au>Carlson, D.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro assay for the biosynthesis and metabolism of juvenile hormone by exposed corpora allata of Aedes aegypti (Diptera: Culicidae)</atitle><jtitle>Journal of medical entomology</jtitle><addtitle>J Med Entomol</addtitle><date>1992-03</date><risdate>1992</risdate><volume>29</volume><issue>2</issue><spage>318</spage><epage>324</epage><pages>318-324</pages><issn>0022-2585</issn><eissn>1938-2928</eissn><coden>JMENA6</coden><abstract>A technique has been developed to study JH III biosynthesis in vitro by removing the head and thorax of Aedes aegypti (L.) and exposing the corpora allata (CA). Exposed CA were incubated with [12-3H] methyl farnesoate, and the newly synthesized JH III and JH III metabolites were followed using C18 reversed-phase, high-performance liquid chromatography (HPLC) and preparative gas chromatography. The rate of synthesis of [12-3H] JH III by exposed CA from newly emerged adult Ae. aegypti was 23 fmol/CA/h. The rate of synthesis of [12-3H] JH III decreased 4-fold 3 d after adult eclosion and increased to 23 fmol/CA/h 5 h after the blood meal. Exposed CA of blood-fed and sugar-fed Ae. aegypti were also incubated with L-[methyl-3H] methionine, and the rate of synthesis of JH III was studied. The rate of JH III biosynthesis increased immediately after the blood meal as was found with [12-3H] methyl farnesoate. The potential application of methyl farnesoate in monitoring the de novo synthesis of JH III in mosquitoes in vitro and in vivo is discussed</abstract><cop>Lanham, MD</cop><pub>Entomological Society of America</pub><pmid>1495050</pmid><doi>10.1093/jmedent/29.2.318</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of medical entomology, 1992-03, Vol.29 (2), p.318-324 |
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| source | MEDLINE; Oxford journals |
| subjects | Aedes - metabolism AEDES AEGYPTI Animals Biochemistry. Physiology. Immunology Biological and medical sciences BIOSINTESIS BIOSYNTHESE Chromatography, Gas Chromatography, High Pressure Liquid Corpora Allata - metabolism Culicidae Diptera Female Fundamental and applied biological sciences. Psychology HORMONAS JUVENILES HORMONE JUVENILE Insecta Invertebrates Juvenile Hormones - biosynthesis Juvenile Hormones - metabolism METABOLISME METABOLISMO Methionine - metabolism Physiology. Development SECRECION SECRETION SISTEMA NERVIOSO SYSTEME NERVEUX |
| title | In vitro assay for the biosynthesis and metabolism of juvenile hormone by exposed corpora allata of Aedes aegypti (Diptera: Culicidae) |
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