ANALYSIS OF THE PRESENCE OF TRYPANOSOMA CRUZI IN THE HEART TISSUE OF THREE PATIENTS WITH CHRONIC CHAGAS' HEART DISEASE
It is still unclear to what extent myocarditis-associated, chronic Chagas' heart disease is due to persisting Trypanosoma cruzi. In the present study, we have analyzed tissue samples from the hearts of three patients with this disease. In situ hybridization provided little evidence for the pres...
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| Veröffentlicht in: | The American journal of tropical medicine and hygiene 2003-02, Vol.68 (2), p.242-247 |
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| container_title | The American journal of tropical medicine and hygiene |
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| creator | ELIAS, FERNANDO E VIGLIANO, CARLOS A LAGUENS, RUBEN P LEVIN, MARIANO J BEREK, CLAUDIA |
| description | It is still unclear to what extent myocarditis-associated, chronic Chagas' heart disease is due to persisting Trypanosoma cruzi. In the present study, we have analyzed tissue samples from the hearts of three patients with this disease. In situ hybridization provided little evidence for the presence of intact T. cruzi even at sites of strong inflammation. Nevertheless, micromanipulation techniques detected remnants of both T. cruzi kinetoplast DNA and nuclear DNA. Trypanosoma cruzi DNA was also detected in single macrophages dissected directly from frozen heart tissue sections. Thus, this analysis demonstrates that T. cruzi kinetoplast DNA and nuclear DNA are widely dispersed in the heart tissue, although in low amounts. Since we rarely detected intact T. cruzi parasites during the chronic phase of Chagas' heart disease, we can exclude heart tissue as a major parasite reservoir. |
| doi_str_mv | 10.4269/ajtmh.2003.68.242 |
| format | Article |
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In the present study, we have analyzed tissue samples from the hearts of three patients with this disease. In situ hybridization provided little evidence for the presence of intact T. cruzi even at sites of strong inflammation. Nevertheless, micromanipulation techniques detected remnants of both T. cruzi kinetoplast DNA and nuclear DNA. Trypanosoma cruzi DNA was also detected in single macrophages dissected directly from frozen heart tissue sections. Thus, this analysis demonstrates that T. cruzi kinetoplast DNA and nuclear DNA are widely dispersed in the heart tissue, although in low amounts. 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In the present study, we have analyzed tissue samples from the hearts of three patients with this disease. In situ hybridization provided little evidence for the presence of intact T. cruzi even at sites of strong inflammation. Nevertheless, micromanipulation techniques detected remnants of both T. cruzi kinetoplast DNA and nuclear DNA. Trypanosoma cruzi DNA was also detected in single macrophages dissected directly from frozen heart tissue sections. Thus, this analysis demonstrates that T. cruzi kinetoplast DNA and nuclear DNA are widely dispersed in the heart tissue, although in low amounts. Since we rarely detected intact T. cruzi parasites during the chronic phase of Chagas' heart disease, we can exclude heart tissue as a major parasite reservoir.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Chagas Cardiomyopathy - parasitology</subject><subject>Chronic Disease</subject><subject>DNA Primers</subject><subject>DNA, Protozoan - genetics</subject><subject>Female</subject><subject>Human protozoal diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Myocarditis - parasitology</subject><subject>Myocardium - pathology</subject><subject>Parasitic diseases</subject><subject>Polymerase Chain Reaction</subject><subject>Protozoal diseases</subject><subject>Tropical medicine</subject><subject>Trypanosoma cruzi - genetics</subject><subject>Trypanosoma cruzi - isolation & purification</subject><subject>Trypanosomiasis</subject><issn>0002-9637</issn><issn>1476-1645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFvmzAYhq1p05p1-wG7TFy2ncj8GYPN0WJuQcqgwlRTd7EMmIWKNC1OFu3fjyRIOe706pOe9_0OD0IfAS8pieJv5nG3WS8JxsEy4ktCySu0AMoiHyIavkYLjDHx4yhgV-idc48YAycAb9EVkIgChXiB_ohcrB5UprzixqtS6d2VUsk8kae7fLgTeaGKH8JLyvtfmZflJyiVoqy8KlPq_gympZyqospkXinvZ1alXpKWRZ4lU4pbob7One-ZkkLJ9-hNZwZnP8x5jaobWSWpvypus0Ss_IYGsPNtw2uITRABB2bazpKWhRQs7wLatk0bc85MzdsAc94ZbuKahXXDAs4a1sU0uEZfzrPP4_Zlb91Ob3rX2GEwT3a7d5oFAITj6L8gcE5YTPAEwhlsxq1zo-3089hvzPhXA9ZHKfokRR-l6IjrScrU-TSP7-uNbS-N2cIEfJ4B4xozdKN5anp34WjIGGXhhVv3v9eHfrTabcwwTLOgD4fD8d3p4T-Hvpik</recordid><startdate>20030201</startdate><enddate>20030201</enddate><creator>ELIAS, FERNANDO E</creator><creator>VIGLIANO, CARLOS A</creator><creator>LAGUENS, RUBEN P</creator><creator>LEVIN, MARIANO J</creator><creator>BEREK, CLAUDIA</creator><general>ASTMH</general><general>Allen Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20030201</creationdate><title>ANALYSIS OF THE PRESENCE OF TRYPANOSOMA CRUZI IN THE HEART TISSUE OF THREE PATIENTS WITH CHRONIC CHAGAS' HEART DISEASE</title><author>ELIAS, FERNANDO E ; VIGLIANO, CARLOS A ; LAGUENS, RUBEN P ; LEVIN, MARIANO J ; BEREK, CLAUDIA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-ec8b19a361817adfe2d7541e8f34ddcd9887ab8d3088fa8a9b75bc7387c7f943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Chagas Cardiomyopathy - parasitology</topic><topic>Chronic Disease</topic><topic>DNA Primers</topic><topic>DNA, Protozoan - genetics</topic><topic>Female</topic><topic>Human protozoal diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>Myocarditis - parasitology</topic><topic>Myocardium - pathology</topic><topic>Parasitic diseases</topic><topic>Polymerase Chain Reaction</topic><topic>Protozoal diseases</topic><topic>Tropical medicine</topic><topic>Trypanosoma cruzi - genetics</topic><topic>Trypanosoma cruzi - isolation & purification</topic><topic>Trypanosomiasis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ELIAS, FERNANDO E</creatorcontrib><creatorcontrib>VIGLIANO, CARLOS A</creatorcontrib><creatorcontrib>LAGUENS, RUBEN P</creatorcontrib><creatorcontrib>LEVIN, MARIANO J</creatorcontrib><creatorcontrib>BEREK, CLAUDIA</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>The American journal of tropical medicine and hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ELIAS, FERNANDO E</au><au>VIGLIANO, CARLOS A</au><au>LAGUENS, RUBEN P</au><au>LEVIN, MARIANO J</au><au>BEREK, CLAUDIA</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ANALYSIS OF THE PRESENCE OF TRYPANOSOMA CRUZI IN THE HEART TISSUE OF THREE PATIENTS WITH CHRONIC CHAGAS' HEART DISEASE</atitle><jtitle>The American journal of tropical medicine and hygiene</jtitle><addtitle>Am J Trop Med Hyg</addtitle><date>2003-02-01</date><risdate>2003</risdate><volume>68</volume><issue>2</issue><spage>242</spage><epage>247</epage><pages>242-247</pages><issn>0002-9637</issn><eissn>1476-1645</eissn><coden>AJTHAB</coden><abstract>It is still unclear to what extent myocarditis-associated, chronic Chagas' heart disease is due to persisting Trypanosoma cruzi. In the present study, we have analyzed tissue samples from the hearts of three patients with this disease. In situ hybridization provided little evidence for the presence of intact T. cruzi even at sites of strong inflammation. Nevertheless, micromanipulation techniques detected remnants of both T. cruzi kinetoplast DNA and nuclear DNA. Trypanosoma cruzi DNA was also detected in single macrophages dissected directly from frozen heart tissue sections. Thus, this analysis demonstrates that T. cruzi kinetoplast DNA and nuclear DNA are widely dispersed in the heart tissue, although in low amounts. Since we rarely detected intact T. cruzi parasites during the chronic phase of Chagas' heart disease, we can exclude heart tissue as a major parasite reservoir.</abstract><cop>Lawrence, KS</cop><pub>ASTMH</pub><pmid>12641419</pmid><doi>10.4269/ajtmh.2003.68.242</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Base Sequence Biological and medical sciences Chagas Cardiomyopathy - parasitology Chronic Disease DNA Primers DNA, Protozoan - genetics Female Human protozoal diseases Humans Infectious diseases Male Medical sciences Mice Mice, Inbred BALB C Middle Aged Molecular Sequence Data Myocarditis - parasitology Myocardium - pathology Parasitic diseases Polymerase Chain Reaction Protozoal diseases Tropical medicine Trypanosoma cruzi - genetics Trypanosoma cruzi - isolation & purification Trypanosomiasis |
| title | ANALYSIS OF THE PRESENCE OF TRYPANOSOMA CRUZI IN THE HEART TISSUE OF THREE PATIENTS WITH CHRONIC CHAGAS' HEART DISEASE |
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