In situ hybridization of GnRH mRNA in the rat and the mink hypothalamus using biotinylated synthetic oligonucleotide probes

The cellular localization of GnRH messenger RNA (mRNA) in the rat and the mink hypothalamus has been examined using a newly developed highly sensitive non-radioactive in situ hybridization procedure. Synthetic oligonucleotides labeled by addition of a biotin-21-dUTP tail at their 3′ end can be used...

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Veröffentlicht in:	Brain research. Molecular brain research. 1992-06, Vol.14 (1), p.57-63
Hauptverfasser:	Boissin-Agasse, Line, de Bouard, Véronique, Roch, Gisèle, Boissin, Jean
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline Phosphatase Animals Bacterial Proteins Base Sequence Biological and medical sciences Biotin Biotinylated oligonucleotide DNA Probes Fundamental and applied biological sciences. Psychology Gonadotropin-Releasing Hormone - biosynthesis Gonadotropin-releasing hormone mRNA Hormones and neuropeptides. Regulation Hypothalamus - metabolism Hypothalamus. Hypophysis. Epiphysis. Urophysis In situ hybridization Male Mink - metabolism Mink hypothalamus Molecular Sequence Data Nucleic Acid Hybridization Rat hypothalamus Rats - metabolism Rats, Inbred Strains RNA, Messenger - analysis Rodentia Seasons Sexual Behavior, Animal Species Specificity Streptavidin Vertebrates: endocrinology
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