Expression of a galactinol synthase gene in tomato seeds is up-regulated before maturation desiccation and again after imbibition whenever radicle protrusion is prevented
Raffinose family oligosaccharides (RFOs) have been implicated in mitigating the effects of environmental stresses on plants. In seeds, proposed roles for RFOs include protecting cellular integrity during desiccation and/or imbibition, extending longevity in the dehydrated state, and providing substr...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2003-03, Vol.131 (3), p.1347-1359 |
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| creator | Downie, B Gurusinghe, S Dahal, P Thacker, R.R Snyder, J.C Nonogaki, H Yim, K Fukanaga, K Alvarado, V Bradford, K.J |
| description | Raffinose family oligosaccharides (RFOs) have been implicated in mitigating the effects of environmental stresses on plants. In seeds, proposed roles for RFOs include protecting cellular integrity during desiccation and/or imbibition, extending longevity in the dehydrated state, and providing substrates for energy generation during germination. A gene encoding galactinol synthase (GOLS), the first committed enzyme in the biosynthesis of RFOs, was cloned from tomato (Lycopersicon esculentum Mill. cv Moneymaker) seeds, and its expression was characterized in tomato seeds and seedlings. GOLS (LeGOLS-1) mRNA accumulated in developing tomato seeds concomitant with maximum dry weight deposition and the acquisition of desiccation tolerance. LeGOLS-1 mRNA was present in mature, desiccated seeds but declined within 8 h of imbibition in wild-type seeds. However, LeGOLS-1 mRNA accumulated again in imbibed seeds prevented from completing germination by dormancy or water deficit. Gibberellin-deficient (gib-1) seeds maintained LeGOLS-1 mRNA amounts after imbibition unless supplied with gibberellin, whereas abscisic acid (ABA) did not prevent the loss of LeGOLS-1 mRNA from wild-type seeds. The presence of LeGOLS-1 mRNA in ABA-deficient (sitiens) tomato seeds indicated that wild-type amounts of ABA are not necessary for its accumulation during seed development. In all cases, LeGOLS-1 mRNA was most prevalent in the radicle tip. LeGOLS-1 mRNA accumulation was induced by dehydration but not by cold in germinating seeds, whereas both stresses induced LeGOLS-1 mRNA accumulation in seedling leaves. The physiological implications of LeGOLS-1 expression patterns in seeds and leaves are discussed in light of the hypothesized role of RFOs in plant stress tolerance. |
| doi_str_mv | 10.1104/pp.016386 |
| format | Article |
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In seeds, proposed roles for RFOs include protecting cellular integrity during desiccation and/or imbibition, extending longevity in the dehydrated state, and providing substrates for energy generation during germination. A gene encoding galactinol synthase (GOLS), the first committed enzyme in the biosynthesis of RFOs, was cloned from tomato (Lycopersicon esculentum Mill. cv Moneymaker) seeds, and its expression was characterized in tomato seeds and seedlings. GOLS (LeGOLS-1) mRNA accumulated in developing tomato seeds concomitant with maximum dry weight deposition and the acquisition of desiccation tolerance. LeGOLS-1 mRNA was present in mature, desiccated seeds but declined within 8 h of imbibition in wild-type seeds. However, LeGOLS-1 mRNA accumulated again in imbibed seeds prevented from completing germination by dormancy or water deficit. Gibberellin-deficient (gib-1) seeds maintained LeGOLS-1 mRNA amounts after imbibition unless supplied with gibberellin, whereas abscisic acid (ABA) did not prevent the loss of LeGOLS-1 mRNA from wild-type seeds. The presence of LeGOLS-1 mRNA in ABA-deficient (sitiens) tomato seeds indicated that wild-type amounts of ABA are not necessary for its accumulation during seed development. In all cases, LeGOLS-1 mRNA was most prevalent in the radicle tip. LeGOLS-1 mRNA accumulation was induced by dehydration but not by cold in germinating seeds, whereas both stresses induced LeGOLS-1 mRNA accumulation in seedling leaves. The physiological implications of LeGOLS-1 expression patterns in seeds and leaves are discussed in light of the hypothesized role of RFOs in plant stress tolerance.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.016386</identifier><identifier>PMID: 12644684</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Biologists</publisher><subject>abscisic acid ; Abscisic Acid - pharmacology ; Adaptation to environment and cultivation conditions ; Agronomy. Soil science and plant productions ; Amino Acid Sequence ; bioaccumulation ; Biological and medical sciences ; biosynthesis ; carbohydrate content ; Cloning, Molecular ; cold stress ; complementary DNA ; Dehydration ; desiccation (plant physiology) ; DNA ; DNA, Complementary - chemistry ; DNA, Complementary - genetics ; Economic plant physiology ; Embryo development. Germination ; Environmental Stress and Adaptation ; enzyme activity ; Fundamental and applied biological sciences. Psychology ; galactosyltransferases ; Galactosyltransferases - genetics ; Galactosyltransferases - metabolism ; gene expression ; Gene Expression Regulation, Developmental - drug effects ; Gene Expression Regulation, Plant - drug effects ; Genetics and breeding of economic plants ; Germination ; Germination - physiology ; Germination and dormancy ; gibberellins ; Gibberellins - pharmacology ; Growth and development ; Imbibition ; inositol 3-alpha-galactosyltransferase ; Leaves ; leGOLS-1 gene ; Lycopersicon esculentum - enzymology ; Lycopersicon esculentum - genetics ; Lycopersicon esculentum - growth & development ; Messenger RNA ; molecular cloning ; Molecular Sequence Data ; Oligosaccharides ; plant anatomy ; Plant physiology and development ; plant proteins ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Roots - genetics ; Plant Roots - growth & development ; Plants ; raffinose ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; seed development ; seed germination ; seed maturation ; Seedlings ; Seeds ; Seeds - enzymology ; Seeds - genetics ; Seeds - growth & development ; Sequence Analysis, DNA ; Solanum lycopersicum var. lycopersicum ; sugars ; Temperature ; tomatoes ; Varietal selection. Specialized plant breeding, plant breeding aims ; vegetable crops ; Water - pharmacology ; water stress</subject><ispartof>Plant physiology (Bethesda), 2003-03, Vol.131 (3), p.1347-1359</ispartof><rights>Copyright 2003 American Society of Plant Biologists</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c525t-6e4c6d9893e84e07758e45afc17d00fa1b07044b71bcbb1df3c299d92c6b572e3</citedby><cites>FETCH-LOGICAL-c525t-6e4c6d9893e84e07758e45afc17d00fa1b07044b71bcbb1df3c299d92c6b572e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4280999$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4280999$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14698894$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12644684$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Downie, B</creatorcontrib><creatorcontrib>Gurusinghe, S</creatorcontrib><creatorcontrib>Dahal, P</creatorcontrib><creatorcontrib>Thacker, R.R</creatorcontrib><creatorcontrib>Snyder, J.C</creatorcontrib><creatorcontrib>Nonogaki, H</creatorcontrib><creatorcontrib>Yim, K</creatorcontrib><creatorcontrib>Fukanaga, K</creatorcontrib><creatorcontrib>Alvarado, V</creatorcontrib><creatorcontrib>Bradford, K.J</creatorcontrib><title>Expression of a galactinol synthase gene in tomato seeds is up-regulated before maturation desiccation and again after imbibition whenever radicle protrusion is prevented</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Raffinose family oligosaccharides (RFOs) have been implicated in mitigating the effects of environmental stresses on plants. In seeds, proposed roles for RFOs include protecting cellular integrity during desiccation and/or imbibition, extending longevity in the dehydrated state, and providing substrates for energy generation during germination. A gene encoding galactinol synthase (GOLS), the first committed enzyme in the biosynthesis of RFOs, was cloned from tomato (Lycopersicon esculentum Mill. cv Moneymaker) seeds, and its expression was characterized in tomato seeds and seedlings. GOLS (LeGOLS-1) mRNA accumulated in developing tomato seeds concomitant with maximum dry weight deposition and the acquisition of desiccation tolerance. LeGOLS-1 mRNA was present in mature, desiccated seeds but declined within 8 h of imbibition in wild-type seeds. However, LeGOLS-1 mRNA accumulated again in imbibed seeds prevented from completing germination by dormancy or water deficit. Gibberellin-deficient (gib-1) seeds maintained LeGOLS-1 mRNA amounts after imbibition unless supplied with gibberellin, whereas abscisic acid (ABA) did not prevent the loss of LeGOLS-1 mRNA from wild-type seeds. The presence of LeGOLS-1 mRNA in ABA-deficient (sitiens) tomato seeds indicated that wild-type amounts of ABA are not necessary for its accumulation during seed development. In all cases, LeGOLS-1 mRNA was most prevalent in the radicle tip. LeGOLS-1 mRNA accumulation was induced by dehydration but not by cold in germinating seeds, whereas both stresses induced LeGOLS-1 mRNA accumulation in seedling leaves. The physiological implications of LeGOLS-1 expression patterns in seeds and leaves are discussed in light of the hypothesized role of RFOs in plant stress tolerance.</description><subject>abscisic acid</subject><subject>Abscisic Acid - pharmacology</subject><subject>Adaptation to environment and cultivation conditions</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Amino Acid Sequence</subject><subject>bioaccumulation</subject><subject>Biological and medical sciences</subject><subject>biosynthesis</subject><subject>carbohydrate content</subject><subject>Cloning, Molecular</subject><subject>cold stress</subject><subject>complementary DNA</subject><subject>Dehydration</subject><subject>desiccation (plant physiology)</subject><subject>DNA</subject><subject>DNA, Complementary - chemistry</subject><subject>DNA, Complementary - genetics</subject><subject>Economic plant physiology</subject><subject>Embryo development. Germination</subject><subject>Environmental Stress and Adaptation</subject><subject>enzyme activity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>galactosyltransferases</subject><subject>Galactosyltransferases - genetics</subject><subject>Galactosyltransferases - metabolism</subject><subject>gene expression</subject><subject>Gene Expression Regulation, Developmental - drug effects</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Genetics and breeding of economic plants</subject><subject>Germination</subject><subject>Germination - physiology</subject><subject>Germination and dormancy</subject><subject>gibberellins</subject><subject>Gibberellins - pharmacology</subject><subject>Growth and development</subject><subject>Imbibition</subject><subject>inositol 3-alpha-galactosyltransferase</subject><subject>Leaves</subject><subject>leGOLS-1 gene</subject><subject>Lycopersicon esculentum - enzymology</subject><subject>Lycopersicon esculentum - genetics</subject><subject>Lycopersicon esculentum - growth & development</subject><subject>Messenger RNA</subject><subject>molecular cloning</subject><subject>Molecular Sequence Data</subject><subject>Oligosaccharides</subject><subject>plant anatomy</subject><subject>Plant physiology and development</subject><subject>plant proteins</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Roots - genetics</subject><subject>Plant Roots - growth & development</subject><subject>Plants</subject><subject>raffinose</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>seed development</subject><subject>seed germination</subject><subject>seed maturation</subject><subject>Seedlings</subject><subject>Seeds</subject><subject>Seeds - enzymology</subject><subject>Seeds - genetics</subject><subject>Seeds - growth & development</subject><subject>Sequence Analysis, DNA</subject><subject>Solanum lycopersicum var. lycopersicum</subject><subject>sugars</subject><subject>Temperature</subject><subject>tomatoes</subject><subject>Varietal selection. Specialized plant breeding, plant breeding aims</subject><subject>vegetable crops</subject><subject>Water - pharmacology</subject><subject>water stress</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1u1TAQhS0EopfCgj0Cb0BikWInjmMvUVV-pEosoOto7IxTV7lxsJ1CX4mnxLe5oktWHvl8OnOkM4S85OyMcyY-LMsZ47JR8hHZ8bapq7oV6jHZMVZmppQ-Ic9SumGM8YaLp-SE11IIqcSO_Ln4vURMyYeZBkeBjjCBzX4OE013c76GhHTEGamfaQ57yIEmxCFRn-i6VBHHdYKMAzXoQkRaiDVCPvgNmLy12wzzQGGEYgIuY6R-b7zx99Kv62J_W_4iDN5OSJcYclzvI5UlJd4tzmXDc_LEwZTwxfE9JVefLn6cf6kuv33-ev7xsrJt3eZKorBy0Eo3qASyrmsVihac5d3AmANuWMeEMB031hg-uMbWWg-6ttK0XY3NKXm3-ZYcP1dMud_7ZHGaYMawpr5rONNcdP8FuZKalWUFfL-BNoaUIrp-iX4P8a7nrD802C9LvzVY2NdH09XscXggj5UV4O0RgGRhchFm69MDJ6QujR-4Vxt3k3KI_3RRK6a1LvKbTXYQehhjsbj6XpcDYZxJIWvV_AXAS7oe</recordid><startdate>20030301</startdate><enddate>20030301</enddate><creator>Downie, B</creator><creator>Gurusinghe, S</creator><creator>Dahal, P</creator><creator>Thacker, R.R</creator><creator>Snyder, J.C</creator><creator>Nonogaki, H</creator><creator>Yim, K</creator><creator>Fukanaga, K</creator><creator>Alvarado, V</creator><creator>Bradford, K.J</creator><general>American Society of Plant Biologists</general><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20030301</creationdate><title>Expression of a galactinol synthase gene in tomato seeds is up-regulated before maturation desiccation and again after imbibition whenever radicle protrusion is prevented</title><author>Downie, B ; Gurusinghe, S ; Dahal, P ; Thacker, R.R ; Snyder, J.C ; Nonogaki, H ; Yim, K ; Fukanaga, K ; Alvarado, V ; Bradford, K.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c525t-6e4c6d9893e84e07758e45afc17d00fa1b07044b71bcbb1df3c299d92c6b572e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>abscisic acid</topic><topic>Abscisic Acid - pharmacology</topic><topic>Adaptation to environment and cultivation conditions</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Amino Acid Sequence</topic><topic>bioaccumulation</topic><topic>Biological and medical sciences</topic><topic>biosynthesis</topic><topic>carbohydrate content</topic><topic>Cloning, Molecular</topic><topic>cold stress</topic><topic>complementary DNA</topic><topic>Dehydration</topic><topic>desiccation (plant physiology)</topic><topic>DNA</topic><topic>DNA, Complementary - chemistry</topic><topic>DNA, Complementary - genetics</topic><topic>Economic plant physiology</topic><topic>Embryo development. Germination</topic><topic>Environmental Stress and Adaptation</topic><topic>enzyme activity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>galactosyltransferases</topic><topic>Galactosyltransferases - genetics</topic><topic>Galactosyltransferases - metabolism</topic><topic>gene expression</topic><topic>Gene Expression Regulation, Developmental - drug effects</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Genetics and breeding of economic plants</topic><topic>Germination</topic><topic>Germination - physiology</topic><topic>Germination and dormancy</topic><topic>gibberellins</topic><topic>Gibberellins - pharmacology</topic><topic>Growth and development</topic><topic>Imbibition</topic><topic>inositol 3-alpha-galactosyltransferase</topic><topic>Leaves</topic><topic>leGOLS-1 gene</topic><topic>Lycopersicon esculentum - enzymology</topic><topic>Lycopersicon esculentum - genetics</topic><topic>Lycopersicon esculentum - growth & development</topic><topic>Messenger RNA</topic><topic>molecular cloning</topic><topic>Molecular Sequence Data</topic><topic>Oligosaccharides</topic><topic>plant anatomy</topic><topic>Plant physiology and development</topic><topic>plant proteins</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Roots - genetics</topic><topic>Plant Roots - growth & development</topic><topic>Plants</topic><topic>raffinose</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>seed development</topic><topic>seed germination</topic><topic>seed maturation</topic><topic>Seedlings</topic><topic>Seeds</topic><topic>Seeds - enzymology</topic><topic>Seeds - genetics</topic><topic>Seeds - growth & development</topic><topic>Sequence Analysis, DNA</topic><topic>Solanum lycopersicum var. lycopersicum</topic><topic>sugars</topic><topic>Temperature</topic><topic>tomatoes</topic><topic>Varietal selection. Specialized plant breeding, plant breeding aims</topic><topic>vegetable crops</topic><topic>Water - pharmacology</topic><topic>water stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Downie, B</creatorcontrib><creatorcontrib>Gurusinghe, S</creatorcontrib><creatorcontrib>Dahal, P</creatorcontrib><creatorcontrib>Thacker, R.R</creatorcontrib><creatorcontrib>Snyder, J.C</creatorcontrib><creatorcontrib>Nonogaki, H</creatorcontrib><creatorcontrib>Yim, K</creatorcontrib><creatorcontrib>Fukanaga, K</creatorcontrib><creatorcontrib>Alvarado, V</creatorcontrib><creatorcontrib>Bradford, K.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Downie, B</au><au>Gurusinghe, S</au><au>Dahal, P</au><au>Thacker, R.R</au><au>Snyder, J.C</au><au>Nonogaki, H</au><au>Yim, K</au><au>Fukanaga, K</au><au>Alvarado, V</au><au>Bradford, K.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of a galactinol synthase gene in tomato seeds is up-regulated before maturation desiccation and again after imbibition whenever radicle protrusion is prevented</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2003-03-01</date><risdate>2003</risdate><volume>131</volume><issue>3</issue><spage>1347</spage><epage>1359</epage><pages>1347-1359</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Raffinose family oligosaccharides (RFOs) have been implicated in mitigating the effects of environmental stresses on plants. In seeds, proposed roles for RFOs include protecting cellular integrity during desiccation and/or imbibition, extending longevity in the dehydrated state, and providing substrates for energy generation during germination. A gene encoding galactinol synthase (GOLS), the first committed enzyme in the biosynthesis of RFOs, was cloned from tomato (Lycopersicon esculentum Mill. cv Moneymaker) seeds, and its expression was characterized in tomato seeds and seedlings. GOLS (LeGOLS-1) mRNA accumulated in developing tomato seeds concomitant with maximum dry weight deposition and the acquisition of desiccation tolerance. LeGOLS-1 mRNA was present in mature, desiccated seeds but declined within 8 h of imbibition in wild-type seeds. However, LeGOLS-1 mRNA accumulated again in imbibed seeds prevented from completing germination by dormancy or water deficit. Gibberellin-deficient (gib-1) seeds maintained LeGOLS-1 mRNA amounts after imbibition unless supplied with gibberellin, whereas abscisic acid (ABA) did not prevent the loss of LeGOLS-1 mRNA from wild-type seeds. The presence of LeGOLS-1 mRNA in ABA-deficient (sitiens) tomato seeds indicated that wild-type amounts of ABA are not necessary for its accumulation during seed development. In all cases, LeGOLS-1 mRNA was most prevalent in the radicle tip. LeGOLS-1 mRNA accumulation was induced by dehydration but not by cold in germinating seeds, whereas both stresses induced LeGOLS-1 mRNA accumulation in seedling leaves. The physiological implications of LeGOLS-1 expression patterns in seeds and leaves are discussed in light of the hypothesized role of RFOs in plant stress tolerance.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Biologists</pub><pmid>12644684</pmid><doi>10.1104/pp.016386</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0889 |
| ispartof | Plant physiology (Bethesda), 2003-03, Vol.131 (3), p.1347-1359 |
| issn | 0032-0889 1532-2548 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73109147 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | abscisic acid Abscisic Acid - pharmacology Adaptation to environment and cultivation conditions Agronomy. Soil science and plant productions Amino Acid Sequence bioaccumulation Biological and medical sciences biosynthesis carbohydrate content Cloning, Molecular cold stress complementary DNA Dehydration desiccation (plant physiology) DNA DNA, Complementary - chemistry DNA, Complementary - genetics Economic plant physiology Embryo development. Germination Environmental Stress and Adaptation enzyme activity Fundamental and applied biological sciences. Psychology galactosyltransferases Galactosyltransferases - genetics Galactosyltransferases - metabolism gene expression Gene Expression Regulation, Developmental - drug effects Gene Expression Regulation, Plant - drug effects Genetics and breeding of economic plants Germination Germination - physiology Germination and dormancy gibberellins Gibberellins - pharmacology Growth and development Imbibition inositol 3-alpha-galactosyltransferase Leaves leGOLS-1 gene Lycopersicon esculentum - enzymology Lycopersicon esculentum - genetics Lycopersicon esculentum - growth & development Messenger RNA molecular cloning Molecular Sequence Data Oligosaccharides plant anatomy Plant physiology and development plant proteins Plant Proteins - genetics Plant Proteins - metabolism Plant Roots - genetics Plant Roots - growth & development Plants raffinose RNA, Messenger - genetics RNA, Messenger - metabolism seed development seed germination seed maturation Seedlings Seeds Seeds - enzymology Seeds - genetics Seeds - growth & development Sequence Analysis, DNA Solanum lycopersicum var. lycopersicum sugars Temperature tomatoes Varietal selection. Specialized plant breeding, plant breeding aims vegetable crops Water - pharmacology water stress |
| title | Expression of a galactinol synthase gene in tomato seeds is up-regulated before maturation desiccation and again after imbibition whenever radicle protrusion is prevented |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T17%3A54%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20a%20galactinol%20synthase%20gene%20in%20tomato%20seeds%20is%20up-regulated%20before%20maturation%20desiccation%20and%20again%20after%20imbibition%20whenever%20radicle%20protrusion%20is%20prevented&rft.jtitle=Plant%20physiology%20(Bethesda)&rft.au=Downie,%20B&rft.date=2003-03-01&rft.volume=131&rft.issue=3&rft.spage=1347&rft.epage=1359&rft.pages=1347-1359&rft.issn=0032-0889&rft.eissn=1532-2548&rft.coden=PPHYA5&rft_id=info:doi/10.1104/pp.016386&rft_dat=%3Cjstor_proqu%3E4280999%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18690070&rft_id=info:pmid/12644684&rft_jstor_id=4280999&rfr_iscdi=true |