Molecular events in the membrane transport of methotrexate in human CCRF-CEM leukemia cell lines

A variant line (CEM-7A) “overproducing” the reduced folate/MTX carrier system was isolated from human CCRF-CEM leukemia cells grown under selective conditions in medium containing 0.25 n m 5-formyl-THF as the sole folate source. This line exhibits a 95-fold increased V max for [ 3H]-MTX influx as co...

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Veröffentlicht in:	Advances in enzyme regulation 1992, Vol.32, p.17-31
Hauptverfasser:	Freisheim, James H., Ratnam, Manohar, McAlinden, Terence P., Prasad, K.M.R., Williams, Frederick E., Robbin Westerhof, G., Schornagel, Jan H., Jansen, Gerrit
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Labels Biological Transport, Active - drug effects Carrier Proteins - metabolism Folate Receptors, GPI-Anchored Folic Acid - analogs & derivatives Humans Leukemia - metabolism Methotrexate - analogs & derivatives Methotrexate - metabolism Purines - pharmacology Receptors, Cell Surface - metabolism Thymidine - pharmacology Tumor Cells, Cultured - metabolism
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