Conjugal transfer of plasmid and transposon DNA from Escherichia coli into Porphyromonas gingivalis
Using the broad host-range vector R751 to provide transfer functions, plasmid pVAL-1 and transposon Tn4351 were conjugally mobilized from Escherichia coli into Porphyromonas gingivalis. Transfer frequencies for both elements varied between 10 −6 and 10 −11, depending upon the recipient. The behavior...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1992-07, Vol.186 (2), p.1012-1019 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Dyer, D.W. Bilalis, G. Michel, J.H. Malek, R. |
| description | Using the broad host-range vector R751 to provide transfer functions, plasmid pVAL-1 and transposon Tn4351 were conjugally mobilized from
Escherichia coli into
Porphyromonas gingivalis. Transfer frequencies for both elements varied between 10
−6 and 10
−11, depending upon the recipient. The behavior of pVAL-1 and Tn4351 in
P. gingivalis was essentially as described previously in
Bacteroides spp. These data indicate that plasmid and transposon DNA can be conjugally transferred into
P. gingivalis and that these elements can be used to genetically manipulate the organism in examining putative virulence determinants that may participate in the induction or exacerbation of periodontal disease. |
| doi_str_mv | 10.1016/0006-291X(92)90847-E |
| format | Article |
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Escherichia coli into
Porphyromonas gingivalis. Transfer frequencies for both elements varied between 10
−6 and 10
−11, depending upon the recipient. The behavior of pVAL-1 and Tn4351 in
P. gingivalis was essentially as described previously in
Bacteroides spp. These data indicate that plasmid and transposon DNA can be conjugally transferred into
P. gingivalis and that these elements can be used to genetically manipulate the organism in examining putative virulence determinants that may participate in the induction or exacerbation of periodontal disease.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(92)90847-E</identifier><identifier>PMID: 1323280</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Autoradiography ; Biological and medical sciences ; Blotting, Southern ; Conjugation, Genetic ; Diverse techniques ; DNA Replication ; DNA Transposable Elements ; DNA, Bacterial - genetics ; DNA, Bacterial - isolation & purification ; DNA, Bacterial - metabolism ; Escherichia coli ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Molecular and cellular biology ; Molecular Weight ; Nucleic Acid Hybridization ; Phosphorus Radioisotopes ; Plasmids ; Porphyromonas gingivalis ; Porphyromonas gingivalis - genetics</subject><ispartof>Biochemical and biophysical research communications, 1992-07, Vol.186 (2), p.1012-1019</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-2df4d4c46bb3ae46cc1e9239e8b214c9ae273125ac219bcdc2eda6a14e3ecd273</citedby><cites>FETCH-LOGICAL-c417t-2df4d4c46bb3ae46cc1e9239e8b214c9ae273125ac219bcdc2eda6a14e3ecd273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(92)90847-E$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5475934$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1323280$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dyer, D.W.</creatorcontrib><creatorcontrib>Bilalis, G.</creatorcontrib><creatorcontrib>Michel, J.H.</creatorcontrib><creatorcontrib>Malek, R.</creatorcontrib><title>Conjugal transfer of plasmid and transposon DNA from Escherichia coli into Porphyromonas gingivalis</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Using the broad host-range vector R751 to provide transfer functions, plasmid pVAL-1 and transposon Tn4351 were conjugally mobilized from
Escherichia coli into
Porphyromonas gingivalis. Transfer frequencies for both elements varied between 10
−6 and 10
−11, depending upon the recipient. The behavior of pVAL-1 and Tn4351 in
P. gingivalis was essentially as described previously in
Bacteroides spp. These data indicate that plasmid and transposon DNA can be conjugally transferred into
P. gingivalis and that these elements can be used to genetically manipulate the organism in examining putative virulence determinants that may participate in the induction or exacerbation of periodontal disease.</description><subject>Autoradiography</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Conjugation, Genetic</subject><subject>Diverse techniques</subject><subject>DNA Replication</subject><subject>DNA Transposable Elements</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>DNA, Bacterial - metabolism</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Molecular and cellular biology</subject><subject>Molecular Weight</subject><subject>Nucleic Acid Hybridization</subject><subject>Phosphorus Radioisotopes</subject><subject>Plasmids</subject><subject>Porphyromonas gingivalis</subject><subject>Porphyromonas gingivalis - genetics</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rFEEQhhtR4rr6DxT6IKKHif01H30RwrpqIEQPCt6anuqa3Q4z3ZPu2UD-fWadJd70VFDvU0XxFCGvOTvnjFcfGWNVITT__V6LD5o1qi62T8iKM80KwZl6SlaPyHPyIucbxjhXlT4jZ1wKKRq2IrCJ4eawsz2dkg25w0RjR8fe5sE7aoNb-mPMMdDP1xe0S3Gg2wx7TB723lKIvac-TJH-iGnc3895DDbTnQ87f2d7n1-SZ53tM7461TX59WX7c_OtuPr-9XJzcVWA4vVUCNcpp0BVbSstqgqAoxZSY9MKrkBbFLXkorQguG7BgUBnK8sVSgQ3Z2vybtk7pnh7wDyZwWfAvrcB4yGbWjLdNKr6L8grqWTJyxlUCwgp5pywM2Pyg033hjNzfII5GjZHw0YL8-cJZjuPvTntP7QDur9Di_U5f3vKbQbbd7Nh8PkRK1Vd6vmCNfm0YDhLu_OYTAaPAdD5hDAZF_2_73gA8SWk9g</recordid><startdate>19920731</startdate><enddate>19920731</enddate><creator>Dyer, D.W.</creator><creator>Bilalis, G.</creator><creator>Michel, J.H.</creator><creator>Malek, R.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19920731</creationdate><title>Conjugal transfer of plasmid and transposon DNA from Escherichia coli into Porphyromonas gingivalis</title><author>Dyer, D.W. ; Bilalis, G. ; Michel, J.H. ; Malek, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-2df4d4c46bb3ae46cc1e9239e8b214c9ae273125ac219bcdc2eda6a14e3ecd273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Autoradiography</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Conjugation, Genetic</topic><topic>Diverse techniques</topic><topic>DNA Replication</topic><topic>DNA Transposable Elements</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>DNA, Bacterial - metabolism</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Molecular and cellular biology</topic><topic>Molecular Weight</topic><topic>Nucleic Acid Hybridization</topic><topic>Phosphorus Radioisotopes</topic><topic>Plasmids</topic><topic>Porphyromonas gingivalis</topic><topic>Porphyromonas gingivalis - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dyer, D.W.</creatorcontrib><creatorcontrib>Bilalis, G.</creatorcontrib><creatorcontrib>Michel, J.H.</creatorcontrib><creatorcontrib>Malek, R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dyer, D.W.</au><au>Bilalis, G.</au><au>Michel, J.H.</au><au>Malek, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Conjugal transfer of plasmid and transposon DNA from Escherichia coli into Porphyromonas gingivalis</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1992-07-31</date><risdate>1992</risdate><volume>186</volume><issue>2</issue><spage>1012</spage><epage>1019</epage><pages>1012-1019</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Using the broad host-range vector R751 to provide transfer functions, plasmid pVAL-1 and transposon Tn4351 were conjugally mobilized from
Escherichia coli into
Porphyromonas gingivalis. Transfer frequencies for both elements varied between 10
−6 and 10
−11, depending upon the recipient. The behavior of pVAL-1 and Tn4351 in
P. gingivalis was essentially as described previously in
Bacteroides spp. These data indicate that plasmid and transposon DNA can be conjugally transferred into
P. gingivalis and that these elements can be used to genetically manipulate the organism in examining putative virulence determinants that may participate in the induction or exacerbation of periodontal disease.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1323280</pmid><doi>10.1016/0006-291X(92)90847-E</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1992-07, Vol.186 (2), p.1012-1019 |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_73098846 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Autoradiography Biological and medical sciences Blotting, Southern Conjugation, Genetic Diverse techniques DNA Replication DNA Transposable Elements DNA, Bacterial - genetics DNA, Bacterial - isolation & purification DNA, Bacterial - metabolism Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Molecular and cellular biology Molecular Weight Nucleic Acid Hybridization Phosphorus Radioisotopes Plasmids Porphyromonas gingivalis Porphyromonas gingivalis - genetics |
| title | Conjugal transfer of plasmid and transposon DNA from Escherichia coli into Porphyromonas gingivalis |
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