The small GTP-binding protein rac regulates growth factor-induced membrane ruffling
The function of rac, a ras-related GTP-binding protein, was investigated in fibroblasts by microinjection. In confluent serum-starved Swiss 3T3 cells, rac1 rapidly stimulated actin filament accumulation at the plasma membrane, forming membrane ruffles. Several growth factors and activated H-ras also...
Gespeichert in:
| Veröffentlicht in: | Cell 1992-08, Vol.70 (3), p.401-410 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 410 |
|---|---|
| container_issue | 3 |
| container_start_page | 401 |
| container_title | Cell |
| container_volume | 70 |
| creator | Ridley, Anne J. Paterson, Hugh F. Johnston, Caroline L. Diekmann, Dagmar Hall, Alan |
| description | The function of rac, a ras-related GTP-binding protein, was investigated in fibroblasts by microinjection. In confluent serum-starved Swiss 3T3 cells, rac1 rapidly stimulated actin filament accumulation at the plasma membrane, forming membrane ruffles. Several growth factors and activated H-ras also induced membrane ruffling, and this response was prevented by a dominant inhibitory mutant rac protein, N17rac1. This suggests that endogenous rac proteins are required for growth factor-induced membrane ruffling. In addition to membrane ruffling, a later response to both rac1 microinjection and some growth factors was the formation of actin stress fibers, a process requiring endogenous rho proteins. Using N17rac1 we have shown that these growth factors act through rac to stimulate this rho-dependent response. We propose that rac and rho are essential components of signal transduction pathways linking growth factors to the organization of polymerized actin. |
| doi_str_mv | 10.1016/0092-8674(92)90164-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73098657</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0092867492901648</els_id><sourcerecordid>16284142</sourcerecordid><originalsourceid>FETCH-LOGICAL-c529t-60a8e68b9b5d22a04449e1897b52d2366af52463d2f1b6177e82057471c8eaff3</originalsourceid><addsrcrecordid>eNqFkF1LHDEUhoNYdLX-gxZyIWIvpk0y-bwRZGlVEFro9jpkMidrZD40mVH675vtLnqnVwfOed7Dy4PQJ0q-UkLlN0IMq7RU_NywL6ZseKX30IISoypOFdtHixfkEB3lfE8I0UKIA3RQ4FoKvUC_V3eAc--6Dl-tflVNHNo4rPFDGieIA07O4wTruXMTZLxO4_N0h4Pz05iqgs4eWtxD3yQ3AE5zCF1Jf0QfgusynOzmMfrz4_tqeV3d_ry6WV7eVl4wM1WSOA1SN6YRLWOOcM4NUG1UI1jLaildEIzLumWBNpIqBZoRobiiXoMLoT5GZ9u_pe3jDHmyfcweuq6UGedsVU2MlkK9C1LJNKecFZBvQZ_GnBME-5Bi79JfS4ndSLcbo3Zj1Jb5X7rVJfZ5939uemhfQ1vL5X66u7vsXReKLR_zCyZ4qWlowS62GBRpTxGSzT7CUBzHBH6y7Rjf7vEPJ4WckQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16284142</pqid></control><display><type>article</type><title>The small GTP-binding protein rac regulates growth factor-induced membrane ruffling</title><source>Elsevier ScienceDirect Journals Complete - AutoHoldings</source><source>MEDLINE</source><creator>Ridley, Anne J. ; Paterson, Hugh F. ; Johnston, Caroline L. ; Diekmann, Dagmar ; Hall, Alan</creator><creatorcontrib>Ridley, Anne J. ; Paterson, Hugh F. ; Johnston, Caroline L. ; Diekmann, Dagmar ; Hall, Alan</creatorcontrib><description>The function of rac, a ras-related GTP-binding protein, was investigated in fibroblasts by microinjection. In confluent serum-starved Swiss 3T3 cells, rac1 rapidly stimulated actin filament accumulation at the plasma membrane, forming membrane ruffles. Several growth factors and activated H-ras also induced membrane ruffling, and this response was prevented by a dominant inhibitory mutant rac protein, N17rac1. This suggests that endogenous rac proteins are required for growth factor-induced membrane ruffling. In addition to membrane ruffling, a later response to both rac1 microinjection and some growth factors was the formation of actin stress fibers, a process requiring endogenous rho proteins. Using N17rac1 we have shown that these growth factors act through rac to stimulate this rho-dependent response. We propose that rac and rho are essential components of signal transduction pathways linking growth factors to the organization of polymerized actin.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/0092-8674(92)90164-8</identifier><identifier>PMID: 1643658</identifier><identifier>CODEN: CELLB5</identifier><language>eng</language><publisher>Cambridge, MA: Elsevier Inc</publisher><subject>3T3 Cells ; actin ; Actins - metabolism ; Animals ; Biological and medical sciences ; Cell Adhesion - physiology ; Cell Membrane - physiology ; Cell physiology ; Culture Media, Serum-Free ; Cytoskeleton - metabolism ; Fluorescent Antibody Technique ; function ; Fundamental and applied biological sciences. Psychology ; growth factors ; Growth Substances - physiology ; GTP-Binding Proteins - genetics ; GTP-Binding Proteins - physiology ; guanine nucleotide-binding protein ; Guanosine Diphosphate - metabolism ; Guanosine Triphosphate - metabolism ; membranes ; Mice ; Molecular and cellular biology ; Oncogene Protein p21(ras) - antagonists & inhibitors ; Oncogene Protein p21(ras) - physiology ; Pinocytosis ; polymerization ; rac GTP-Binding Proteins ; Rac protein ; Responses to growth factors, tumor promotors, other factors ; Substrate Specificity ; Swiss 3T3 cells</subject><ispartof>Cell, 1992-08, Vol.70 (3), p.401-410</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c529t-60a8e68b9b5d22a04449e1897b52d2366af52463d2f1b6177e82057471c8eaff3</citedby><cites>FETCH-LOGICAL-c529t-60a8e68b9b5d22a04449e1897b52d2366af52463d2f1b6177e82057471c8eaff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0092-8674(92)90164-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5498691$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1643658$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ridley, Anne J.</creatorcontrib><creatorcontrib>Paterson, Hugh F.</creatorcontrib><creatorcontrib>Johnston, Caroline L.</creatorcontrib><creatorcontrib>Diekmann, Dagmar</creatorcontrib><creatorcontrib>Hall, Alan</creatorcontrib><title>The small GTP-binding protein rac regulates growth factor-induced membrane ruffling</title><title>Cell</title><addtitle>Cell</addtitle><description>The function of rac, a ras-related GTP-binding protein, was investigated in fibroblasts by microinjection. In confluent serum-starved Swiss 3T3 cells, rac1 rapidly stimulated actin filament accumulation at the plasma membrane, forming membrane ruffles. Several growth factors and activated H-ras also induced membrane ruffling, and this response was prevented by a dominant inhibitory mutant rac protein, N17rac1. This suggests that endogenous rac proteins are required for growth factor-induced membrane ruffling. In addition to membrane ruffling, a later response to both rac1 microinjection and some growth factors was the formation of actin stress fibers, a process requiring endogenous rho proteins. Using N17rac1 we have shown that these growth factors act through rac to stimulate this rho-dependent response. We propose that rac and rho are essential components of signal transduction pathways linking growth factors to the organization of polymerized actin.</description><subject>3T3 Cells</subject><subject>actin</subject><subject>Actins - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Adhesion - physiology</subject><subject>Cell Membrane - physiology</subject><subject>Cell physiology</subject><subject>Culture Media, Serum-Free</subject><subject>Cytoskeleton - metabolism</subject><subject>Fluorescent Antibody Technique</subject><subject>function</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>growth factors</subject><subject>Growth Substances - physiology</subject><subject>GTP-Binding Proteins - genetics</subject><subject>GTP-Binding Proteins - physiology</subject><subject>guanine nucleotide-binding protein</subject><subject>Guanosine Diphosphate - metabolism</subject><subject>Guanosine Triphosphate - metabolism</subject><subject>membranes</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Oncogene Protein p21(ras) - antagonists & inhibitors</subject><subject>Oncogene Protein p21(ras) - physiology</subject><subject>Pinocytosis</subject><subject>polymerization</subject><subject>rac GTP-Binding Proteins</subject><subject>Rac protein</subject><subject>Responses to growth factors, tumor promotors, other factors</subject><subject>Substrate Specificity</subject><subject>Swiss 3T3 cells</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkF1LHDEUhoNYdLX-gxZyIWIvpk0y-bwRZGlVEFro9jpkMidrZD40mVH675vtLnqnVwfOed7Dy4PQJ0q-UkLlN0IMq7RU_NywL6ZseKX30IISoypOFdtHixfkEB3lfE8I0UKIA3RQ4FoKvUC_V3eAc--6Dl-tflVNHNo4rPFDGieIA07O4wTruXMTZLxO4_N0h4Pz05iqgs4eWtxD3yQ3AE5zCF1Jf0QfgusynOzmMfrz4_tqeV3d_ry6WV7eVl4wM1WSOA1SN6YRLWOOcM4NUG1UI1jLaildEIzLumWBNpIqBZoRobiiXoMLoT5GZ9u_pe3jDHmyfcweuq6UGedsVU2MlkK9C1LJNKecFZBvQZ_GnBME-5Bi79JfS4ndSLcbo3Zj1Jb5X7rVJfZ5939uemhfQ1vL5X66u7vsXReKLR_zCyZ4qWlowS62GBRpTxGSzT7CUBzHBH6y7Rjf7vEPJ4WckQ</recordid><startdate>19920807</startdate><enddate>19920807</enddate><creator>Ridley, Anne J.</creator><creator>Paterson, Hugh F.</creator><creator>Johnston, Caroline L.</creator><creator>Diekmann, Dagmar</creator><creator>Hall, Alan</creator><general>Elsevier Inc</general><general>Cell Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19920807</creationdate><title>The small GTP-binding protein rac regulates growth factor-induced membrane ruffling</title><author>Ridley, Anne J. ; Paterson, Hugh F. ; Johnston, Caroline L. ; Diekmann, Dagmar ; Hall, Alan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c529t-60a8e68b9b5d22a04449e1897b52d2366af52463d2f1b6177e82057471c8eaff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>3T3 Cells</topic><topic>actin</topic><topic>Actins - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Adhesion - physiology</topic><topic>Cell Membrane - physiology</topic><topic>Cell physiology</topic><topic>Culture Media, Serum-Free</topic><topic>Cytoskeleton - metabolism</topic><topic>Fluorescent Antibody Technique</topic><topic>function</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>growth factors</topic><topic>Growth Substances - physiology</topic><topic>GTP-Binding Proteins - genetics</topic><topic>GTP-Binding Proteins - physiology</topic><topic>guanine nucleotide-binding protein</topic><topic>Guanosine Diphosphate - metabolism</topic><topic>Guanosine Triphosphate - metabolism</topic><topic>membranes</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Oncogene Protein p21(ras) - antagonists & inhibitors</topic><topic>Oncogene Protein p21(ras) - physiology</topic><topic>Pinocytosis</topic><topic>polymerization</topic><topic>rac GTP-Binding Proteins</topic><topic>Rac protein</topic><topic>Responses to growth factors, tumor promotors, other factors</topic><topic>Substrate Specificity</topic><topic>Swiss 3T3 cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ridley, Anne J.</creatorcontrib><creatorcontrib>Paterson, Hugh F.</creatorcontrib><creatorcontrib>Johnston, Caroline L.</creatorcontrib><creatorcontrib>Diekmann, Dagmar</creatorcontrib><creatorcontrib>Hall, Alan</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ridley, Anne J.</au><au>Paterson, Hugh F.</au><au>Johnston, Caroline L.</au><au>Diekmann, Dagmar</au><au>Hall, Alan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The small GTP-binding protein rac regulates growth factor-induced membrane ruffling</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>1992-08-07</date><risdate>1992</risdate><volume>70</volume><issue>3</issue><spage>401</spage><epage>410</epage><pages>401-410</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><coden>CELLB5</coden><abstract>The function of rac, a ras-related GTP-binding protein, was investigated in fibroblasts by microinjection. In confluent serum-starved Swiss 3T3 cells, rac1 rapidly stimulated actin filament accumulation at the plasma membrane, forming membrane ruffles. Several growth factors and activated H-ras also induced membrane ruffling, and this response was prevented by a dominant inhibitory mutant rac protein, N17rac1. This suggests that endogenous rac proteins are required for growth factor-induced membrane ruffling. In addition to membrane ruffling, a later response to both rac1 microinjection and some growth factors was the formation of actin stress fibers, a process requiring endogenous rho proteins. Using N17rac1 we have shown that these growth factors act through rac to stimulate this rho-dependent response. We propose that rac and rho are essential components of signal transduction pathways linking growth factors to the organization of polymerized actin.</abstract><cop>Cambridge, MA</cop><pub>Elsevier Inc</pub><pmid>1643658</pmid><doi>10.1016/0092-8674(92)90164-8</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0092-8674 |
| ispartof | Cell, 1992-08, Vol.70 (3), p.401-410 |
| issn | 0092-8674 1097-4172 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73098657 |
| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | 3T3 Cells actin Actins - metabolism Animals Biological and medical sciences Cell Adhesion - physiology Cell Membrane - physiology Cell physiology Culture Media, Serum-Free Cytoskeleton - metabolism Fluorescent Antibody Technique function Fundamental and applied biological sciences. Psychology growth factors Growth Substances - physiology GTP-Binding Proteins - genetics GTP-Binding Proteins - physiology guanine nucleotide-binding protein Guanosine Diphosphate - metabolism Guanosine Triphosphate - metabolism membranes Mice Molecular and cellular biology Oncogene Protein p21(ras) - antagonists & inhibitors Oncogene Protein p21(ras) - physiology Pinocytosis polymerization rac GTP-Binding Proteins Rac protein Responses to growth factors, tumor promotors, other factors Substrate Specificity Swiss 3T3 cells |
| title | The small GTP-binding protein rac regulates growth factor-induced membrane ruffling |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T16%3A02%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20small%20GTP-binding%20protein%20rac%20regulates%20growth%20factor-induced%20membrane%20ruffling&rft.jtitle=Cell&rft.au=Ridley,%20Anne%20J.&rft.date=1992-08-07&rft.volume=70&rft.issue=3&rft.spage=401&rft.epage=410&rft.pages=401-410&rft.issn=0092-8674&rft.eissn=1097-4172&rft.coden=CELLB5&rft_id=info:doi/10.1016/0092-8674(92)90164-8&rft_dat=%3Cproquest_cross%3E16284142%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16284142&rft_id=info:pmid/1643658&rft_els_id=0092867492901648&rfr_iscdi=true |