Production and molecular characteristics of four groups of exopolysaccharides from submerged culture of Phellinus gilvus
Aims: The objective of the present study was to determine the optimal culture conditions for the production of four groups of exopolysaccharides (EPSs) in Phellinus gilvus by submerged culture and to investigate their molecular properties by multi‐angle laser‐light scattering (MALLS) analysis. Metho...
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| creator | Hwang, H.J. Kim, S.W. Xu, C.P. Choi, J.W. Yun, J.W. |
| description | Aims: The objective of the present study was to determine the optimal culture conditions for the production of four groups of exopolysaccharides (EPSs) in Phellinus gilvus by submerged culture and to investigate their molecular properties by multi‐angle laser‐light scattering (MALLS) analysis.
Methods and Results: The optimal temperature and initial pH for the production of both mycelial biomass and EPSs in P. gilvus by submerged flask cultures were found to be 30°C and pH 9·0, respectively. Glucose and corn steep powder were the most suitable carbon and nitrogen source for both mycelial biomass and EPS production. Optimal medium composition was determined to be glucose 30 g l−1, corn steep powder 5 g l−1, MgSO4 1·23 g l−1, KH2PO4 0·68 g l−1, and K2HPO4 0·87 g l−1. Four groups of EPSs (Fr‐I, II, III, and IV) were obtained from the culture filtrates by gel filtration chromatography on Sepharose CL‐4B and characterized by size exclusion chromatography (SEC) coupled with MALLS. The weight average molar mass (Mw) of Fr‐I, Fr‐II, Fr‐III and Fr‐IV were determined to be 8·628 × 106 (±129 420), 1·045 × 106 (±19 855), 61·09 × 104 (±1244), and 33·55 × 104 (±134) g mol−1, respectively.
Conclusions: Under optimal culture conditions, the maximum EPS production in a 5‐l stirred fermenter indicated 5·3 g l−1 after 11 days of fermentation. The SEC/MALLS analysis revealed that Fr‐I, which has extremely high molecular weight, was presumably an aggregate of complex polysaccharides forming a compact globular shape; whereas Fr‐II was nearly spherical, Fr‐III and Fr‐IV were rod‐like chains in an aqueous solution.
Significance and Impact of the Study: This is the first report on the production of high amounts of EPSs from liquid‐culture of the basidiomycete, P. gilvus. The SEC/MALLS approach used in this study could be useful in providing greater insight into the characterization of the mushroom polysaccharides without carrying out elaborate fractionation procedures prior to analysis. |
| doi_str_mv | 10.1046/j.1365-2672.2003.01903.x |
| format | Article |
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Methods and Results: The optimal temperature and initial pH for the production of both mycelial biomass and EPSs in P. gilvus by submerged flask cultures were found to be 30°C and pH 9·0, respectively. Glucose and corn steep powder were the most suitable carbon and nitrogen source for both mycelial biomass and EPS production. Optimal medium composition was determined to be glucose 30 g l−1, corn steep powder 5 g l−1, MgSO4 1·23 g l−1, KH2PO4 0·68 g l−1, and K2HPO4 0·87 g l−1. Four groups of EPSs (Fr‐I, II, III, and IV) were obtained from the culture filtrates by gel filtration chromatography on Sepharose CL‐4B and characterized by size exclusion chromatography (SEC) coupled with MALLS. The weight average molar mass (Mw) of Fr‐I, Fr‐II, Fr‐III and Fr‐IV were determined to be 8·628 × 106 (±129 420), 1·045 × 106 (±19 855), 61·09 × 104 (±1244), and 33·55 × 104 (±134) g mol−1, respectively.
Conclusions: Under optimal culture conditions, the maximum EPS production in a 5‐l stirred fermenter indicated 5·3 g l−1 after 11 days of fermentation. The SEC/MALLS analysis revealed that Fr‐I, which has extremely high molecular weight, was presumably an aggregate of complex polysaccharides forming a compact globular shape; whereas Fr‐II was nearly spherical, Fr‐III and Fr‐IV were rod‐like chains in an aqueous solution.
Significance and Impact of the Study: This is the first report on the production of high amounts of EPSs from liquid‐culture of the basidiomycete, P. gilvus. The SEC/MALLS approach used in this study could be useful in providing greater insight into the characterization of the mushroom polysaccharides without carrying out elaborate fractionation procedures prior to analysis.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1046/j.1365-2672.2003.01903.x</identifier><identifier>PMID: 12631207</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Amino Acids - analysis ; Biological and medical sciences ; Biomass ; Biotechnology ; Carbohydrates - analysis ; Carbon - pharmacology ; Chromatography, Gel ; Culture Media ; exopolysaccharide ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Methods. Procedures. Technologies ; Microbial engineering. Fermentation and microbial culture technology ; Molecular Weight ; mycelial biomass ; Mycelium - growth & development ; Mycology - methods ; Nitrogen - pharmacology ; Phellinus gilvus ; Polyporales - drug effects ; Polyporales - growth & development ; Polyporales - metabolism ; Polysaccharides - biosynthesis ; Polysaccharides - chemistry ; Polysaccharides - isolation & purification ; Scattering, Radiation ; submerged culture ; Temperature</subject><ispartof>Journal of applied microbiology, 2003-01, Vol.94 (4), p.708-719</ispartof><rights>2003 INIST-CNRS</rights><rights>Copyright Blackwell Science Ltd. 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5033-8defb41c5f410bdd64f2ac829e774c983b619fe7a68adeb6de8bb48666989aa23</citedby><cites>FETCH-LOGICAL-c5033-8defb41c5f410bdd64f2ac829e774c983b619fe7a68adeb6de8bb48666989aa23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2672.2003.01903.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2672.2003.01903.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14663616$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12631207$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hwang, H.J.</creatorcontrib><creatorcontrib>Kim, S.W.</creatorcontrib><creatorcontrib>Xu, C.P.</creatorcontrib><creatorcontrib>Choi, J.W.</creatorcontrib><creatorcontrib>Yun, J.W.</creatorcontrib><title>Production and molecular characteristics of four groups of exopolysaccharides from submerged culture of Phellinus gilvus</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims: The objective of the present study was to determine the optimal culture conditions for the production of four groups of exopolysaccharides (EPSs) in Phellinus gilvus by submerged culture and to investigate their molecular properties by multi‐angle laser‐light scattering (MALLS) analysis.
Methods and Results: The optimal temperature and initial pH for the production of both mycelial biomass and EPSs in P. gilvus by submerged flask cultures were found to be 30°C and pH 9·0, respectively. Glucose and corn steep powder were the most suitable carbon and nitrogen source for both mycelial biomass and EPS production. Optimal medium composition was determined to be glucose 30 g l−1, corn steep powder 5 g l−1, MgSO4 1·23 g l−1, KH2PO4 0·68 g l−1, and K2HPO4 0·87 g l−1. Four groups of EPSs (Fr‐I, II, III, and IV) were obtained from the culture filtrates by gel filtration chromatography on Sepharose CL‐4B and characterized by size exclusion chromatography (SEC) coupled with MALLS. The weight average molar mass (Mw) of Fr‐I, Fr‐II, Fr‐III and Fr‐IV were determined to be 8·628 × 106 (±129 420), 1·045 × 106 (±19 855), 61·09 × 104 (±1244), and 33·55 × 104 (±134) g mol−1, respectively.
Conclusions: Under optimal culture conditions, the maximum EPS production in a 5‐l stirred fermenter indicated 5·3 g l−1 after 11 days of fermentation. The SEC/MALLS analysis revealed that Fr‐I, which has extremely high molecular weight, was presumably an aggregate of complex polysaccharides forming a compact globular shape; whereas Fr‐II was nearly spherical, Fr‐III and Fr‐IV were rod‐like chains in an aqueous solution.
Significance and Impact of the Study: This is the first report on the production of high amounts of EPSs from liquid‐culture of the basidiomycete, P. gilvus. The SEC/MALLS approach used in this study could be useful in providing greater insight into the characterization of the mushroom polysaccharides without carrying out elaborate fractionation procedures prior to analysis.</description><subject>Amino Acids - analysis</subject><subject>Biological and medical sciences</subject><subject>Biomass</subject><subject>Biotechnology</subject><subject>Carbohydrates - analysis</subject><subject>Carbon - pharmacology</subject><subject>Chromatography, Gel</subject><subject>Culture Media</subject><subject>exopolysaccharide</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial engineering. Fermentation and microbial culture technology</subject><subject>Molecular Weight</subject><subject>mycelial biomass</subject><subject>Mycelium - growth & development</subject><subject>Mycology - methods</subject><subject>Nitrogen - pharmacology</subject><subject>Phellinus gilvus</subject><subject>Polyporales - drug effects</subject><subject>Polyporales - growth & development</subject><subject>Polyporales - metabolism</subject><subject>Polysaccharides - biosynthesis</subject><subject>Polysaccharides - chemistry</subject><subject>Polysaccharides - isolation & purification</subject><subject>Scattering, Radiation</subject><subject>submerged culture</subject><subject>Temperature</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtvEzEUhS0EoiXwF5CFBLsJfsz4sWBRVTxVRBewtjx-pI5mxsEel-Tf40kiKrGBjX2v_N2j63MAgBitMWrZ2-0aU9Y1hHGyJgjRNcKynvtH4PLPw-Nj3TYd4uQCPMt5ixCmqGNPwQUmjGKC-CXY36Zoi5lDnKCeLBzj4EwZdILmTidtZpdCnoPJMHroY0lwk2LZHVu3j7s4HLI2Cxusy9CnOMJc-tGljbOwKs0luQW-vXPDEKaS4SYM9yU_B0-8HrJ7cb5X4MeH99-vPzU33z5-vr66aUyHKG2Edb5vsel8i1FvLWs90UYQ6ThvjRS0Z1h6xzUT2rqeWSf6vhWMMSmk1oSuwJuT7i7Fn8XlWY0hm7qLnlwsWXGKpEAU_RPEgmNOqoUr8OovcFt9meonFKFEdpJRWiFxgkyKOSfn1S6FUaeDwkgtGaqtWqJSS1RqyVAdM1T7OvryrL_4aB8Gz6FV4PUZ0NnowSc9mZAfuJYxyjCr3LsT9ysM7vDfC6gvV1-Xiv4G7vu52w</recordid><startdate>20030101</startdate><enddate>20030101</enddate><creator>Hwang, H.J.</creator><creator>Kim, S.W.</creator><creator>Xu, C.P.</creator><creator>Choi, J.W.</creator><creator>Yun, J.W.</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20030101</creationdate><title>Production and molecular characteristics of four groups of exopolysaccharides from submerged culture of Phellinus gilvus</title><author>Hwang, H.J. ; Kim, S.W. ; Xu, C.P. ; Choi, J.W. ; Yun, J.W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5033-8defb41c5f410bdd64f2ac829e774c983b619fe7a68adeb6de8bb48666989aa23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Amino Acids - analysis</topic><topic>Biological and medical sciences</topic><topic>Biomass</topic><topic>Biotechnology</topic><topic>Carbohydrates - analysis</topic><topic>Carbon - pharmacology</topic><topic>Chromatography, Gel</topic><topic>Culture Media</topic><topic>exopolysaccharide</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbial engineering. Fermentation and microbial culture technology</topic><topic>Molecular Weight</topic><topic>mycelial biomass</topic><topic>Mycelium - growth & development</topic><topic>Mycology - methods</topic><topic>Nitrogen - pharmacology</topic><topic>Phellinus gilvus</topic><topic>Polyporales - drug effects</topic><topic>Polyporales - growth & development</topic><topic>Polyporales - metabolism</topic><topic>Polysaccharides - biosynthesis</topic><topic>Polysaccharides - chemistry</topic><topic>Polysaccharides - isolation & purification</topic><topic>Scattering, Radiation</topic><topic>submerged culture</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hwang, H.J.</creatorcontrib><creatorcontrib>Kim, S.W.</creatorcontrib><creatorcontrib>Xu, C.P.</creatorcontrib><creatorcontrib>Choi, J.W.</creatorcontrib><creatorcontrib>Yun, J.W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hwang, H.J.</au><au>Kim, S.W.</au><au>Xu, C.P.</au><au>Choi, J.W.</au><au>Yun, J.W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production and molecular characteristics of four groups of exopolysaccharides from submerged culture of Phellinus gilvus</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2003-01-01</date><risdate>2003</risdate><volume>94</volume><issue>4</issue><spage>708</spage><epage>719</epage><pages>708-719</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aims: The objective of the present study was to determine the optimal culture conditions for the production of four groups of exopolysaccharides (EPSs) in Phellinus gilvus by submerged culture and to investigate their molecular properties by multi‐angle laser‐light scattering (MALLS) analysis.
Methods and Results: The optimal temperature and initial pH for the production of both mycelial biomass and EPSs in P. gilvus by submerged flask cultures were found to be 30°C and pH 9·0, respectively. Glucose and corn steep powder were the most suitable carbon and nitrogen source for both mycelial biomass and EPS production. Optimal medium composition was determined to be glucose 30 g l−1, corn steep powder 5 g l−1, MgSO4 1·23 g l−1, KH2PO4 0·68 g l−1, and K2HPO4 0·87 g l−1. Four groups of EPSs (Fr‐I, II, III, and IV) were obtained from the culture filtrates by gel filtration chromatography on Sepharose CL‐4B and characterized by size exclusion chromatography (SEC) coupled with MALLS. The weight average molar mass (Mw) of Fr‐I, Fr‐II, Fr‐III and Fr‐IV were determined to be 8·628 × 106 (±129 420), 1·045 × 106 (±19 855), 61·09 × 104 (±1244), and 33·55 × 104 (±134) g mol−1, respectively.
Conclusions: Under optimal culture conditions, the maximum EPS production in a 5‐l stirred fermenter indicated 5·3 g l−1 after 11 days of fermentation. The SEC/MALLS analysis revealed that Fr‐I, which has extremely high molecular weight, was presumably an aggregate of complex polysaccharides forming a compact globular shape; whereas Fr‐II was nearly spherical, Fr‐III and Fr‐IV were rod‐like chains in an aqueous solution.
Significance and Impact of the Study: This is the first report on the production of high amounts of EPSs from liquid‐culture of the basidiomycete, P. gilvus. The SEC/MALLS approach used in this study could be useful in providing greater insight into the characterization of the mushroom polysaccharides without carrying out elaborate fractionation procedures prior to analysis.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>12631207</pmid><doi>10.1046/j.1365-2672.2003.01903.x</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acids - analysis Biological and medical sciences Biomass Biotechnology Carbohydrates - analysis Carbon - pharmacology Chromatography, Gel Culture Media exopolysaccharide Fermentation Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Molecular Weight mycelial biomass Mycelium - growth & development Mycology - methods Nitrogen - pharmacology Phellinus gilvus Polyporales - drug effects Polyporales - growth & development Polyporales - metabolism Polysaccharides - biosynthesis Polysaccharides - chemistry Polysaccharides - isolation & purification Scattering, Radiation submerged culture Temperature |
| title | Production and molecular characteristics of four groups of exopolysaccharides from submerged culture of Phellinus gilvus |
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