Dr1, a TATA-binding protein-associated phosphoprotein and inhibitor of class II gene transcription
We have discovered a protein termed Dr1 that interacts with the TATA-binding protein, TBP. The association of Dr1 with TBP results in repression of both basal and activated levels of transcription. The interaction of Dr1 with TBP precludes the formation of a transcription-competent complex by inhibi...
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| Veröffentlicht in: | Cell 1992-08, Vol.70 (3), p.477-489 |
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| creator | Inostroza, A. Mermelstein, Fred H. Ha, Ilho Lane, William S. Reinberg, Danny |
| description | We have discovered a protein termed Dr1 that interacts with the TATA-binding protein, TBP. The association of Dr1 with TBP results in repression of both basal and activated levels of transcription. The interaction of Dr1 with TBP precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Dr1 activity is associated with a 19 kd protein. A cDNA clone encoding Dr1 was isolated. Dr1 is phosphorylated in vivo and phosphorylation of Dr1 affected its interaction with TBP. Our results suggest a regulatory role for Dr1 in repression of transcription mediated via phosphorylation. |
| doi_str_mv | 10.1016/0092-8674(92)90172-9 |
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The association of Dr1 with TBP results in repression of both basal and activated levels of transcription. The interaction of Dr1 with TBP precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Dr1 activity is associated with a 19 kd protein. A cDNA clone encoding Dr1 was isolated. Dr1 is phosphorylated in vivo and phosphorylation of Dr1 affected its interaction with TBP. Our results suggest a regulatory role for Dr1 in repression of transcription mediated via phosphorylation.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/0092-8674(92)90172-9</identifier><identifier>PMID: 1339312</identifier><identifier>CODEN: CELLB5</identifier><language>eng</language><publisher>Cambridge, MA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Base Sequence ; Biological and medical sciences ; Blotting, Western ; cDNA ; Cloning, Molecular ; DNA ; DNA-Binding Proteins - metabolism ; Dr1 protein ; Electrophoresis, Polyacrylamide Gel ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; genes ; Genes, MHC Class II ; HeLa Cells ; Humans ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; nucleotide sequence ; Phosphoproteins - genetics ; Phosphoproteins - metabolism ; Phosphorylation ; Precipitin Tests ; predictions ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; repression ; TATA-binding protein ; TATA-Box Binding Protein ; transcription ; Transcription Factor TFIIA ; Transcription Factor TFIIB ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription, Genetic ; Transcription. Transcription factor. Splicing. Rna processing</subject><ispartof>Cell, 1992-08, Vol.70 (3), p.477-489</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c529t-e8646f3c109c4383da08c6e70d4618b96a01988c42ffd65f3f746e409151563</citedby><cites>FETCH-LOGICAL-c529t-e8646f3c109c4383da08c6e70d4618b96a01988c42ffd65f3f746e409151563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0092867492901729$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5498699$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1339312$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inostroza, A.</creatorcontrib><creatorcontrib>Mermelstein, Fred H.</creatorcontrib><creatorcontrib>Ha, Ilho</creatorcontrib><creatorcontrib>Lane, William S.</creatorcontrib><creatorcontrib>Reinberg, Danny</creatorcontrib><title>Dr1, a TATA-binding protein-associated phosphoprotein and inhibitor of class II gene transcription</title><title>Cell</title><addtitle>Cell</addtitle><description>We have discovered a protein termed Dr1 that interacts with the TATA-binding protein, TBP. The association of Dr1 with TBP results in repression of both basal and activated levels of transcription. The interaction of Dr1 with TBP precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Dr1 activity is associated with a 19 kd protein. A cDNA clone encoding Dr1 was isolated. Dr1 is phosphorylated in vivo and phosphorylation of Dr1 affected its interaction with TBP. Our results suggest a regulatory role for Dr1 in repression of transcription mediated via phosphorylation.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>cDNA</subject><subject>Cloning, Molecular</subject><subject>DNA</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Dr1 protein</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>genes</subject><subject>Genes, MHC Class II</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequence</subject><subject>Phosphoproteins - genetics</subject><subject>Phosphoproteins - metabolism</subject><subject>Phosphorylation</subject><subject>Precipitin Tests</subject><subject>predictions</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>repression</subject><subject>TATA-binding protein</subject><subject>TATA-Box Binding Protein</subject><subject>transcription</subject><subject>Transcription Factor TFIIA</subject><subject>Transcription Factor TFIIB</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><subject>Transcription. 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Psychology</topic><topic>Gene Expression Regulation</topic><topic>genes</topic><topic>Genes, MHC Class II</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequence</topic><topic>Phosphoproteins - genetics</topic><topic>Phosphoproteins - metabolism</topic><topic>Phosphorylation</topic><topic>Precipitin Tests</topic><topic>predictions</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>repression</topic><topic>TATA-binding protein</topic><topic>TATA-Box Binding Protein</topic><topic>transcription</topic><topic>Transcription Factor TFIIA</topic><topic>Transcription Factor TFIIB</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Inostroza, A.</creatorcontrib><creatorcontrib>Mermelstein, Fred H.</creatorcontrib><creatorcontrib>Ha, Ilho</creatorcontrib><creatorcontrib>Lane, William S.</creatorcontrib><creatorcontrib>Reinberg, Danny</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Human Genome Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inostroza, A.</au><au>Mermelstein, Fred H.</au><au>Ha, Ilho</au><au>Lane, William S.</au><au>Reinberg, Danny</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dr1, a TATA-binding protein-associated phosphoprotein and inhibitor of class II gene transcription</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>1992-08-07</date><risdate>1992</risdate><volume>70</volume><issue>3</issue><spage>477</spage><epage>489</epage><pages>477-489</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><coden>CELLB5</coden><abstract>We have discovered a protein termed Dr1 that interacts with the TATA-binding protein, TBP. The association of Dr1 with TBP results in repression of both basal and activated levels of transcription. The interaction of Dr1 with TBP precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Dr1 activity is associated with a 19 kd protein. A cDNA clone encoding Dr1 was isolated. Dr1 is phosphorylated in vivo and phosphorylation of Dr1 affected its interaction with TBP. Our results suggest a regulatory role for Dr1 in repression of transcription mediated via phosphorylation.</abstract><cop>Cambridge, MA</cop><pub>Elsevier Inc</pub><pmid>1339312</pmid><doi>10.1016/0092-8674(92)90172-9</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Base Sequence Biological and medical sciences Blotting, Western cDNA Cloning, Molecular DNA DNA-Binding Proteins - metabolism Dr1 protein Electrophoresis, Polyacrylamide Gel Escherichia coli Fundamental and applied biological sciences. Psychology Gene Expression Regulation genes Genes, MHC Class II HeLa Cells Humans Molecular and cellular biology Molecular genetics Molecular Sequence Data nucleotide sequence Phosphoproteins - genetics Phosphoproteins - metabolism Phosphorylation Precipitin Tests predictions Recombinant Proteins - genetics Recombinant Proteins - metabolism repression TATA-binding protein TATA-Box Binding Protein transcription Transcription Factor TFIIA Transcription Factor TFIIB Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing |
| title | Dr1, a TATA-binding protein-associated phosphoprotein and inhibitor of class II gene transcription |
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