Molecular mapping of mouse chromosomes 4 and 6: Use of a flow-sorted robertsonian chromosome
The development of dense genetic maps of mammalian chromosomes is facilitated when chromosome-specific libraries are used as a source of genetic markers. To saturate the genetic maps of mouse chromosomes 4 and 6, we have made use of fluorescent-activated chromosome sorting to purify a 4:6 Robertsoni...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 1992-07, Vol.13 (3), p.761-769 |
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| container_title | Genomics (San Diego, Calif.) |
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| creator | Bahary, Nathan Pachter, Jane E. Felman, Rina Leibel, Rudolph L. Albright, Kevin Cram, Scott Friedman, Jeffrey M. |
| description | The development of dense genetic maps of mammalian chromosomes is facilitated when chromosome-specific libraries are used as a source of genetic markers. To saturate the genetic maps of mouse chromosomes 4 and 6, we have made use of fluorescent-activated chromosome sorting to purify a 4:6 Robertsonian chromosome from a cell line harboring the Rb(4:6)2Bnr translocation. After staining with chromomycin A3 and Hoechst 33528, this chromosome was separated from the other mouse chromosomes. DNA was isolated from the fraction containing the Robertsonian chromosome and subcloned into the insertion vector λgt 10, generating a library with 4.6 × 10
5 independent phage. A total of 19 single-copy sequences were used to type the progeny of a C57BL/6J ×
Mus spretus backcross that had previously been typed for loci on chromosomes 4 and 6. Approximately 70% of the clones in the library mapped to either chromosome 4 or 6 as assessed by genetic mapping and by use of a somatic cell hybrid panel. Simple sequence repeats have also been isolated from this library. Further characterization of these microsatellites should accelerate efforts to map mouse chromosomes 4 and 6 using PCR. In addition, flow sorting of Robertsonian chromosomes suggests a general approach for making chromosome-specific libraries in mouse. |
| doi_str_mv | 10.1016/0888-7543(92)90151-H |
| format | Article |
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5 independent phage. A total of 19 single-copy sequences were used to type the progeny of a C57BL/6J ×
Mus spretus backcross that had previously been typed for loci on chromosomes 4 and 6. Approximately 70% of the clones in the library mapped to either chromosome 4 or 6 as assessed by genetic mapping and by use of a somatic cell hybrid panel. Simple sequence repeats have also been isolated from this library. Further characterization of these microsatellites should accelerate efforts to map mouse chromosomes 4 and 6 using PCR. In addition, flow sorting of Robertsonian chromosomes suggests a general approach for making chromosome-specific libraries in mouse.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1016/0888-7543(92)90151-H</identifier><identifier>PMID: 1639403</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Cell Line ; Chromosome Mapping - methods ; Classical genetics, quantitative genetics, hybrids ; Cloning, Molecular ; DNA - genetics ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; Genetic Linkage ; Genetic Markers ; Genetics of eukaryotes. Biological and molecular evolution ; Genomic Library ; Mice - genetics ; Mice, Inbred C57BL ; Muridae ; Pedigree ; Polymerase Chain Reaction ; Repetitive Sequences, Nucleic Acid ; Translocation, Genetic ; Vertebrata</subject><ispartof>Genomics (San Diego, Calif.), 1992-07, Vol.13 (3), p.761-769</ispartof><rights>1992 Academic Press, Inc.</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-9d415a4b45208a07795bf6c63e90db746c2ce1b2fe9af3467612cda055f73b693</citedby><cites>FETCH-LOGICAL-c332t-9d415a4b45208a07795bf6c63e90db746c2ce1b2fe9af3467612cda055f73b693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/088875439290151H$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5360960$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1639403$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bahary, Nathan</creatorcontrib><creatorcontrib>Pachter, Jane E.</creatorcontrib><creatorcontrib>Felman, Rina</creatorcontrib><creatorcontrib>Leibel, Rudolph L.</creatorcontrib><creatorcontrib>Albright, Kevin</creatorcontrib><creatorcontrib>Cram, Scott</creatorcontrib><creatorcontrib>Friedman, Jeffrey M.</creatorcontrib><title>Molecular mapping of mouse chromosomes 4 and 6: Use of a flow-sorted robertsonian chromosome</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>The development of dense genetic maps of mammalian chromosomes is facilitated when chromosome-specific libraries are used as a source of genetic markers. To saturate the genetic maps of mouse chromosomes 4 and 6, we have made use of fluorescent-activated chromosome sorting to purify a 4:6 Robertsonian chromosome from a cell line harboring the Rb(4:6)2Bnr translocation. After staining with chromomycin A3 and Hoechst 33528, this chromosome was separated from the other mouse chromosomes. DNA was isolated from the fraction containing the Robertsonian chromosome and subcloned into the insertion vector λgt 10, generating a library with 4.6 × 10
5 independent phage. A total of 19 single-copy sequences were used to type the progeny of a C57BL/6J ×
Mus spretus backcross that had previously been typed for loci on chromosomes 4 and 6. Approximately 70% of the clones in the library mapped to either chromosome 4 or 6 as assessed by genetic mapping and by use of a somatic cell hybrid panel. Simple sequence repeats have also been isolated from this library. Further characterization of these microsatellites should accelerate efforts to map mouse chromosomes 4 and 6 using PCR. In addition, flow sorting of Robertsonian chromosomes suggests a general approach for making chromosome-specific libraries in mouse.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Chromosome Mapping - methods</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Cloning, Molecular</subject><subject>DNA - genetics</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Linkage</subject><subject>Genetic Markers</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Genomic Library</subject><subject>Mice - genetics</subject><subject>Mice, Inbred C57BL</subject><subject>Muridae</subject><subject>Pedigree</subject><subject>Polymerase Chain Reaction</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Translocation, Genetic</subject><subject>Vertebrata</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2LFDEQhoMo67j6DxRyENFDa-Wzkz0IsqgjrHhxb0JIpysa6e6MSY_ivzfjDKsnPdXhfd6i6iHkIYPnDJh-AcaYrldSPLX8mQWmWLe9RTYMjO2Mlvo22dwgd8m9Wr8CgBWGn5EzpoWVIDbk0_s8YdhPvtDZ73Zp-UxzpHPeV6ThS8lzrnnGSiX1y0j1Bb1uQSM8jVP-0dVcVhxpyQOWteYl-eWv2n1yJ_qp4oPTPCfXb15_vNx2Vx_evrt8ddUFIfja2VEy5eUgFQfjoe-tGqIOWqCFceilDjwgG3hE66OQuteMh9GDUrEXg7binDw57t2V_G2PdXVzqgGnyS_YPnG9AMsNU_8Fmeamb9IaKI9gKLnWgtHtSpp9-ekYuIN9d1DrDmqd5e63fbdttUen_fthxvFP6ai75Y9Pua_BT7H4JaR6gymhwWpo2Msjhk3a94TF1ZBwCTimgmF1Y07_vuMXwRWgFA</recordid><startdate>199207</startdate><enddate>199207</enddate><creator>Bahary, Nathan</creator><creator>Pachter, Jane E.</creator><creator>Felman, Rina</creator><creator>Leibel, Rudolph L.</creator><creator>Albright, Kevin</creator><creator>Cram, Scott</creator><creator>Friedman, Jeffrey M.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199207</creationdate><title>Molecular mapping of mouse chromosomes 4 and 6: Use of a flow-sorted robertsonian chromosome</title><author>Bahary, Nathan ; Pachter, Jane E. ; Felman, Rina ; Leibel, Rudolph L. ; Albright, Kevin ; Cram, Scott ; Friedman, Jeffrey M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-9d415a4b45208a07795bf6c63e90db746c2ce1b2fe9af3467612cda055f73b693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Chromosome Mapping - methods</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Cloning, Molecular</topic><topic>DNA - genetics</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Linkage</topic><topic>Genetic Markers</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Genomic Library</topic><topic>Mice - genetics</topic><topic>Mice, Inbred C57BL</topic><topic>Muridae</topic><topic>Pedigree</topic><topic>Polymerase Chain Reaction</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Translocation, Genetic</topic><topic>Vertebrata</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bahary, Nathan</creatorcontrib><creatorcontrib>Pachter, Jane E.</creatorcontrib><creatorcontrib>Felman, Rina</creatorcontrib><creatorcontrib>Leibel, Rudolph L.</creatorcontrib><creatorcontrib>Albright, Kevin</creatorcontrib><creatorcontrib>Cram, Scott</creatorcontrib><creatorcontrib>Friedman, Jeffrey M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bahary, Nathan</au><au>Pachter, Jane E.</au><au>Felman, Rina</au><au>Leibel, Rudolph L.</au><au>Albright, Kevin</au><au>Cram, Scott</au><au>Friedman, Jeffrey M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular mapping of mouse chromosomes 4 and 6: Use of a flow-sorted robertsonian chromosome</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>1992-07</date><risdate>1992</risdate><volume>13</volume><issue>3</issue><spage>761</spage><epage>769</epage><pages>761-769</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>The development of dense genetic maps of mammalian chromosomes is facilitated when chromosome-specific libraries are used as a source of genetic markers. To saturate the genetic maps of mouse chromosomes 4 and 6, we have made use of fluorescent-activated chromosome sorting to purify a 4:6 Robertsonian chromosome from a cell line harboring the Rb(4:6)2Bnr translocation. After staining with chromomycin A3 and Hoechst 33528, this chromosome was separated from the other mouse chromosomes. DNA was isolated from the fraction containing the Robertsonian chromosome and subcloned into the insertion vector λgt 10, generating a library with 4.6 × 10
5 independent phage. A total of 19 single-copy sequences were used to type the progeny of a C57BL/6J ×
Mus spretus backcross that had previously been typed for loci on chromosomes 4 and 6. Approximately 70% of the clones in the library mapped to either chromosome 4 or 6 as assessed by genetic mapping and by use of a somatic cell hybrid panel. Simple sequence repeats have also been isolated from this library. Further characterization of these microsatellites should accelerate efforts to map mouse chromosomes 4 and 6 using PCR. In addition, flow sorting of Robertsonian chromosomes suggests a general approach for making chromosome-specific libraries in mouse.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1639403</pmid><doi>10.1016/0888-7543(92)90151-H</doi><tpages>9</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Biological and medical sciences Cell Line Chromosome Mapping - methods Classical genetics, quantitative genetics, hybrids Cloning, Molecular DNA - genetics Flow Cytometry Fundamental and applied biological sciences. Psychology Genetic Linkage Genetic Markers Genetics of eukaryotes. Biological and molecular evolution Genomic Library Mice - genetics Mice, Inbred C57BL Muridae Pedigree Polymerase Chain Reaction Repetitive Sequences, Nucleic Acid Translocation, Genetic Vertebrata |
| title | Molecular mapping of mouse chromosomes 4 and 6: Use of a flow-sorted robertsonian chromosome |
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