Comparison of the susceptibility testing of clinical isolates of Mycobacterium tuberculosis by the XTT colorimetric method and the NCCLS standards method
The susceptibility or resistance of clinical isolates of Mycobacterium tuberculosis were determined by a method incorporating the 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-carboxanilide (XTT) and compared with results obtained by the National Committee for Clinical Laboratory Standa...
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| Veröffentlicht in: | International journal of antimicrobial agents 2003-03, Vol.21 (3), p.244-250 |
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| container_title | International journal of antimicrobial agents |
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| creator | De Logu, Alessandro Pellerano, Maria Luisa Sanna, Adriana Pusceddu, Maria Cristina Uda, Patrizia Saddi, Barbara |
| description | The susceptibility or resistance of clinical isolates of
Mycobacterium tuberculosis were determined by a method incorporating the 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-carboxanilide (XTT) and compared with results obtained by the National Committee for Clinical Laboratory Standards approved standard method (M24-T2). One hundred percent of all isolates demonstrated agreement between the susceptibility and resistance to isoniazid, rifampicin, and ethambutol obtained by the two methods, suggesting that the XTT-based method could provide a useful means for the rapid determination of antimycobacterial susceptibility of clinical isolates of
M. tuberculosis. |
| doi_str_mv | 10.1016/S0924-8579(02)00350-3 |
| format | Article |
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Mycobacterium tuberculosis were determined by a method incorporating the 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-carboxanilide (XTT) and compared with results obtained by the National Committee for Clinical Laboratory Standards approved standard method (M24-T2). One hundred percent of all isolates demonstrated agreement between the susceptibility and resistance to isoniazid, rifampicin, and ethambutol obtained by the two methods, suggesting that the XTT-based method could provide a useful means for the rapid determination of antimycobacterial susceptibility of clinical isolates of
M. tuberculosis.</description><subject>Antibacterial agents</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antitubercular Agents - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Colony Count, Microbial</subject><subject>Colorimetric methods</subject><subject>Colorimetry - methods</subject><subject>Drug Resistance, Bacterial</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Medical sciences</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>Microbial Sensitivity Tests - standards</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - drug effects</subject><subject>Mycobacterium tuberculosis - growth & development</subject><subject>Mycobacterium tuberculosis - isolation & purification</subject><subject>Pharmacology. 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| subjects | Antibacterial agents Antibiotics. Antiinfectious agents. Antiparasitic agents Antitubercular Agents - pharmacology Biological and medical sciences Colony Count, Microbial Colorimetric methods Colorimetry - methods Drug Resistance, Bacterial Humans In Vitro Techniques Medical sciences Microbial Sensitivity Tests - methods Microbial Sensitivity Tests - standards Mycobacterium tuberculosis Mycobacterium tuberculosis - drug effects Mycobacterium tuberculosis - growth & development Mycobacterium tuberculosis - isolation & purification Pharmacology. Drug treatments Susceptibility testing Tetrazolium Salts Tuberculosis, Multidrug-Resistant - drug therapy Tuberculosis, Multidrug-Resistant - microbiology XTT |
| title | Comparison of the susceptibility testing of clinical isolates of Mycobacterium tuberculosis by the XTT colorimetric method and the NCCLS standards method |
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