Codon context effect in virus translational readthrough A study in vitro of the determinants of TMV and Mo-MuLV amber suppression
To assess the role of codon context on the efficiency of eukaryotic suppression of termination codons, we have compared, in a rabbit cell-free translation system, the readthrough efficiency related to two synthetic transcripts differing by the codon context around an amber codon. The codon contexts...
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| Veröffentlicht in: | FEBS letters 1992-07, Vol.306 (2), p.133-139 |
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| creator | Valle, Rosaura P.C. Drugeon, Gabrièle Devignes-Morch, Marie-Dominique Legocki, Andrzej B. Haenni, Anne-Lise |
| description | To assess the role of codon context on the efficiency of eukaryotic suppression of termination codons, we have compared, in a rabbit cell-free translation system, the readthrough efficiency related to two synthetic transcripts differing by the codon context around an amber codon. The codon contexts are derived from tobacco mosaic virus (TMV) and Moloney murine leukemia virus (Mo-MuLV) RNAs. The Mo-MuLV-like codon context does not promote suppression. Substituting TMV-derived triplets in the Mo-MuLV-like codon context shows that the two codons downstrem from the TMV UAG signal are important determinants of suppression, as recently demonstrated in vivo. |
| doi_str_mv | 10.1016/0014-5793(92)80984-O |
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The codon contexts are derived from tobacco mosaic virus (TMV) and Moloney murine leukemia virus (Mo-MuLV) RNAs. The Mo-MuLV-like codon context does not promote suppression. Substituting TMV-derived triplets in the Mo-MuLV-like codon context shows that the two codons downstrem from the TMV UAG signal are important determinants of suppression, as recently demonstrated in vivo.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(92)80984-O</identifier><identifier>PMID: 1321731</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; CAT ; Cell-Free System ; chloramphenicol acetyltransferase ; Chloramphenicol O-Acetyltransferase - genetics ; Chloramphenicol O-Acetyltransferase - metabolism ; Cloning, Molecular ; Codon ; Codon context. Amber codon ; DNA, Viral ; Electrophoresis, Polyacrylamide Gel ; Fundamental and applied biological sciences. Psychology ; Genes, Synthetic ; Leukemia Virus, Murine - genetics ; Mo-MuLV ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Moloney murine leukemia virus ; Nonsense suppression ; nucleotide ; open reading frame ; ORF ; Protein Biosynthesis ; Rabbits ; Regulation of translation ; release factor ; RNA virus ; RNA, Viral ; Terminator Regions, Genetic - genetics ; TMV ; tobacco mosaic virus ; Tobacco Mosaic Virus - genetics ; Translation. Translation factors. Protein processing ; turnip yellow mosaic virus ; TYMV ; Viral gene expression</subject><ispartof>FEBS letters, 1992-07, Vol.306 (2), p.133-139</ispartof><rights>1992</rights><rights>FEBS Letters 306 (1992) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430O-7619e14fc3cd7f8b65f0b899ecfcc79e301dea22c9d0f728e13183dfdfd4db7c3</citedby><cites>FETCH-LOGICAL-c430O-7619e14fc3cd7f8b65f0b899ecfcc79e301dea22c9d0f728e13183dfdfd4db7c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/001457939280984O$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5399701$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1321731$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valle, Rosaura P.C.</creatorcontrib><creatorcontrib>Drugeon, Gabrièle</creatorcontrib><creatorcontrib>Devignes-Morch, Marie-Dominique</creatorcontrib><creatorcontrib>Legocki, Andrzej B.</creatorcontrib><creatorcontrib>Haenni, Anne-Lise</creatorcontrib><title>Codon context effect in virus translational readthrough A study in vitro of the determinants of TMV and Mo-MuLV amber suppression</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>To assess the role of codon context on the efficiency of eukaryotic suppression of termination codons, we have compared, in a rabbit cell-free translation system, the readthrough efficiency related to two synthetic transcripts differing by the codon context around an amber codon. The codon contexts are derived from tobacco mosaic virus (TMV) and Moloney murine leukemia virus (Mo-MuLV) RNAs. The Mo-MuLV-like codon context does not promote suppression. Substituting TMV-derived triplets in the Mo-MuLV-like codon context shows that the two codons downstrem from the TMV UAG signal are important determinants of suppression, as recently demonstrated in vivo.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>CAT</subject><subject>Cell-Free System</subject><subject>chloramphenicol acetyltransferase</subject><subject>Chloramphenicol O-Acetyltransferase - genetics</subject><subject>Chloramphenicol O-Acetyltransferase - metabolism</subject><subject>Cloning, Molecular</subject><subject>Codon</subject><subject>Codon context. Amber codon</subject><subject>DNA, Viral</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Synthetic</subject><subject>Leukemia Virus, Murine - genetics</subject><subject>Mo-MuLV</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Moloney murine leukemia virus</subject><subject>Nonsense suppression</subject><subject>nucleotide</subject><subject>open reading frame</subject><subject>ORF</subject><subject>Protein Biosynthesis</subject><subject>Rabbits</subject><subject>Regulation of translation</subject><subject>release factor</subject><subject>RNA virus</subject><subject>RNA, Viral</subject><subject>Terminator Regions, Genetic - genetics</subject><subject>TMV</subject><subject>tobacco mosaic virus</subject><subject>Tobacco Mosaic Virus - genetics</subject><subject>Translation. Translation factors. Protein processing</subject><subject>turnip yellow mosaic virus</subject><subject>TYMV</subject><subject>Viral gene expression</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUGPEyEYhidGs9bVf6AJB2P0MArDtMDFZG22atKml9UrYeDDYmagArPao_9cZqdZb8ZwAN7v-V7gpaqeE_yWYLJ6hzFp6yUT9LVo3nAseFvvH1QLwhmtabviD6vFPfK4epLSd1z2nIiL6oLQhjBKFtXvdTDBIx18hl8ZgbWgM3Ie3bo4JpSj8qlX2QWvehRBmXyIYfx2QFco5dGcZjTHgIJF-QDIQIY4OK98TpN2s_uKlDdoF-rduC3roYOI0ng8Rkip-D6tHlnVJ3h2ni-rL5vrm_Wnerv_-Hl9ta11S_G-ZisigLRWU22Y5d1qaXHHhQBttWYCKCYGVNNoYbBlDQdCCafGltGajml6Wb2afY8x_BghZTm4pKHvlYcwJsko5qQVbQHbGdQxpBTBymN0g4onSbCckpdTrHKKVYpG3iUv96Xtxdl_7AYwf5vmqEv95bmukla9Lclql-6xJRWC4QnbzNhP18Ppv46Wm-sPzVSYdNHcqdN93s9GUEK9dRBl0g68BuNi-WNpgvv3g_4AqSi1dw</recordid><startdate>19920720</startdate><enddate>19920720</enddate><creator>Valle, Rosaura P.C.</creator><creator>Drugeon, Gabrièle</creator><creator>Devignes-Morch, Marie-Dominique</creator><creator>Legocki, Andrzej B.</creator><creator>Haenni, Anne-Lise</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920720</creationdate><title>Codon context effect in virus translational readthrough A study in vitro of the determinants of TMV and Mo-MuLV amber suppression</title><author>Valle, Rosaura P.C. ; Drugeon, Gabrièle ; Devignes-Morch, Marie-Dominique ; Legocki, Andrzej B. ; Haenni, Anne-Lise</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430O-7619e14fc3cd7f8b65f0b899ecfcc79e301dea22c9d0f728e13183dfdfd4db7c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>CAT</topic><topic>Cell-Free System</topic><topic>chloramphenicol acetyltransferase</topic><topic>Chloramphenicol O-Acetyltransferase - genetics</topic><topic>Chloramphenicol O-Acetyltransferase - metabolism</topic><topic>Cloning, Molecular</topic><topic>Codon</topic><topic>Codon context. Amber codon</topic><topic>DNA, Viral</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Synthetic</topic><topic>Leukemia Virus, Murine - genetics</topic><topic>Mo-MuLV</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Moloney murine leukemia virus</topic><topic>Nonsense suppression</topic><topic>nucleotide</topic><topic>open reading frame</topic><topic>ORF</topic><topic>Protein Biosynthesis</topic><topic>Rabbits</topic><topic>Regulation of translation</topic><topic>release factor</topic><topic>RNA virus</topic><topic>RNA, Viral</topic><topic>Terminator Regions, Genetic - genetics</topic><topic>TMV</topic><topic>tobacco mosaic virus</topic><topic>Tobacco Mosaic Virus - genetics</topic><topic>Translation. Translation factors. Protein processing</topic><topic>turnip yellow mosaic virus</topic><topic>TYMV</topic><topic>Viral gene expression</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valle, Rosaura P.C.</creatorcontrib><creatorcontrib>Drugeon, Gabrièle</creatorcontrib><creatorcontrib>Devignes-Morch, Marie-Dominique</creatorcontrib><creatorcontrib>Legocki, Andrzej B.</creatorcontrib><creatorcontrib>Haenni, Anne-Lise</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valle, Rosaura P.C.</au><au>Drugeon, Gabrièle</au><au>Devignes-Morch, Marie-Dominique</au><au>Legocki, Andrzej B.</au><au>Haenni, Anne-Lise</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Codon context effect in virus translational readthrough A study in vitro of the determinants of TMV and Mo-MuLV amber suppression</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1992-07-20</date><risdate>1992</risdate><volume>306</volume><issue>2</issue><spage>133</spage><epage>139</epage><pages>133-139</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>To assess the role of codon context on the efficiency of eukaryotic suppression of termination codons, we have compared, in a rabbit cell-free translation system, the readthrough efficiency related to two synthetic transcripts differing by the codon context around an amber codon. The codon contexts are derived from tobacco mosaic virus (TMV) and Moloney murine leukemia virus (Mo-MuLV) RNAs. The Mo-MuLV-like codon context does not promote suppression. Substituting TMV-derived triplets in the Mo-MuLV-like codon context shows that the two codons downstrem from the TMV UAG signal are important determinants of suppression, as recently demonstrated in vivo.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1321731</pmid><doi>10.1016/0014-5793(92)80984-O</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1992-07, Vol.306 (2), p.133-139 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences CAT Cell-Free System chloramphenicol acetyltransferase Chloramphenicol O-Acetyltransferase - genetics Chloramphenicol O-Acetyltransferase - metabolism Cloning, Molecular Codon Codon context. Amber codon DNA, Viral Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Genes, Synthetic Leukemia Virus, Murine - genetics Mo-MuLV Molecular and cellular biology Molecular genetics Molecular Sequence Data Moloney murine leukemia virus Nonsense suppression nucleotide open reading frame ORF Protein Biosynthesis Rabbits Regulation of translation release factor RNA virus RNA, Viral Terminator Regions, Genetic - genetics TMV tobacco mosaic virus Tobacco Mosaic Virus - genetics Translation. Translation factors. Protein processing turnip yellow mosaic virus TYMV Viral gene expression |
| title | Codon context effect in virus translational readthrough A study in vitro of the determinants of TMV and Mo-MuLV amber suppression |
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