Human kallikrein 2 (hk2) mRNA in peripheral blood of patients with thyroid cancer: A novel molecular marker?
To elucidate whether hK2 mRNA can be detected in peripheral blood of patients with thyroid disease using reverse transcription polymerase chain reaction (RT-PCR). A nested RT-PCR protocol for the detection of hK2 mRNA was established, and blood samples of 72 patients with a history of thyroid cancer...
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| Veröffentlicht in: | Journal of cancer research and clinical oncology 2003, Vol.129 (1), p.29-34 |
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| container_title | Journal of cancer research and clinical oncology |
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| creator | RODDIGER, Sandra J RENNEBERG, Heiner MARTIN, Thomas TUNN, Ulf W ZAMBOGLOU, Nikolaos KUREK, Ralf |
| description | To elucidate whether hK2 mRNA can be detected in peripheral blood of patients with thyroid disease using reverse transcription polymerase chain reaction (RT-PCR).
A nested RT-PCR protocol for the detection of hK2 mRNA was established, and blood samples of 72 patients with a history of thyroid cancer, 10 patients with current metastases of thyroid cancer, and 32 volunteers were tested.
hK2-transcripts were significantly more often detected in patients with thyroid cancer (20/72=28%) than in the control group (2/32=6%, P = 0.03, chi-square analysis).
This is the first study reporting on hK2 as a potential molecular marker for patients with thyroid cancer. We could demonstrate a correlation between diagnosis of thyroid cancer and the positive signal for hK2 in the RT-PCR assay. Future studies are necessary to prove the clinical value of hK2 as a molecular marker regarding recurrence and outcome. |
| doi_str_mv | 10.1007/s00432-002-0401-y |
| format | Article |
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A nested RT-PCR protocol for the detection of hK2 mRNA was established, and blood samples of 72 patients with a history of thyroid cancer, 10 patients with current metastases of thyroid cancer, and 32 volunteers were tested.
hK2-transcripts were significantly more often detected in patients with thyroid cancer (20/72=28%) than in the control group (2/32=6%, P = 0.03, chi-square analysis).
This is the first study reporting on hK2 as a potential molecular marker for patients with thyroid cancer. We could demonstrate a correlation between diagnosis of thyroid cancer and the positive signal for hK2 in the RT-PCR assay. Future studies are necessary to prove the clinical value of hK2 as a molecular marker regarding recurrence and outcome.</description><identifier>ISSN: 0171-5216</identifier><identifier>EISSN: 1432-1335</identifier><identifier>DOI: 10.1007/s00432-002-0401-y</identifier><identifier>PMID: 12618898</identifier><identifier>CODEN: JCROD7</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Adult ; Aged ; Biological and medical sciences ; Biomarkers, Tumor - blood ; Biomarkers, Tumor - genetics ; Blood ; Carcinoma, Papillary - blood ; DNA, Complementary - analysis ; Endocrinopathies ; Female ; Humans ; Male ; Malignant tumors ; Medical sciences ; Middle Aged ; Predictive Value of Tests ; Reverse Transcriptase Polymerase Chain Reaction ; Ribonucleic acid ; RNA ; Sensitivity and Specificity ; Thyroid cancer ; Thyroid gland ; Thyroid Neoplasms - blood ; Thyroid. Thyroid axis (diseases) ; Tissue Kallikreins - blood ; Tissue Kallikreins - genetics</subject><ispartof>Journal of cancer research and clinical oncology, 2003, Vol.129 (1), p.29-34</ispartof><rights>2003 INIST-CNRS</rights><rights>Copyright Springer-Verlag 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c306t-6bc8464a9ef19645307a9ce3f31393cc05428f5cc3ca58bb09783e89823cf2b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14642035$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12618898$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>RODDIGER, Sandra J</creatorcontrib><creatorcontrib>RENNEBERG, Heiner</creatorcontrib><creatorcontrib>MARTIN, Thomas</creatorcontrib><creatorcontrib>TUNN, Ulf W</creatorcontrib><creatorcontrib>ZAMBOGLOU, Nikolaos</creatorcontrib><creatorcontrib>KUREK, Ralf</creatorcontrib><title>Human kallikrein 2 (hk2) mRNA in peripheral blood of patients with thyroid cancer: A novel molecular marker?</title><title>Journal of cancer research and clinical oncology</title><addtitle>J Cancer Res Clin Oncol</addtitle><description>To elucidate whether hK2 mRNA can be detected in peripheral blood of patients with thyroid disease using reverse transcription polymerase chain reaction (RT-PCR).
A nested RT-PCR protocol for the detection of hK2 mRNA was established, and blood samples of 72 patients with a history of thyroid cancer, 10 patients with current metastases of thyroid cancer, and 32 volunteers were tested.
hK2-transcripts were significantly more often detected in patients with thyroid cancer (20/72=28%) than in the control group (2/32=6%, P = 0.03, chi-square analysis).
This is the first study reporting on hK2 as a potential molecular marker for patients with thyroid cancer. We could demonstrate a correlation between diagnosis of thyroid cancer and the positive signal for hK2 in the RT-PCR assay. Future studies are necessary to prove the clinical value of hK2 as a molecular marker regarding recurrence and outcome.</description><subject>Adult</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - blood</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Blood</subject><subject>Carcinoma, Papillary - blood</subject><subject>DNA, Complementary - analysis</subject><subject>Endocrinopathies</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Malignant tumors</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Predictive Value of Tests</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Sensitivity and Specificity</subject><subject>Thyroid cancer</subject><subject>Thyroid gland</subject><subject>Thyroid Neoplasms - blood</subject><subject>Thyroid. Thyroid axis (diseases)</subject><subject>Tissue Kallikreins - blood</subject><subject>Tissue Kallikreins - genetics</subject><issn>0171-5216</issn><issn>1432-1335</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkU1rHDEMhk1JaTZJf0AuwQRa2sMksjWfvZQlNE0gpFDSs_F4NayznvHUnmnZfx8vuxDIQQiJR0KvXsbOBVwJgOo6AuQoM4AUOYhs-44txK4jEIsjtgBRiayQojxmJzE-Q6qLSn5gx0KWoq6besHc3dzrgW-0c3YTyA5c8i_rjfzK-9-PS57qkYId1xS0463zfsV9x0c9WRqmyP_bac2n9TZ4u-JGD4bCN77kg_9HjvfekZmdDrzXYUPh-xl732kX6eMhn7I_tz-ebu6yh18_72-WD5lBKKesbE2dl7luqBNNmRcIlW4MYYcCGzQGilzWXWEMGl3UbQtNVSMlORJNJ1uJp-zzfu8Y_N-Z4qR6Gw05pwfyc1QVQg3YNAm8fAM--zkM6TYlJYgylzkmSOwhE3yMgTo1BpsUbZUAtfNB7X1QyQe180Ft08zFYfHc9rR6nTg8PgGfDoCORrsupN_Z-Mol_RKwwBeUII6r</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>RODDIGER, Sandra J</creator><creator>RENNEBERG, Heiner</creator><creator>MARTIN, Thomas</creator><creator>TUNN, Ulf W</creator><creator>ZAMBOGLOU, Nikolaos</creator><creator>KUREK, Ralf</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>2003</creationdate><title>Human kallikrein 2 (hk2) mRNA in peripheral blood of patients with thyroid cancer: A novel molecular marker?</title><author>RODDIGER, Sandra J ; RENNEBERG, Heiner ; MARTIN, Thomas ; TUNN, Ulf W ; ZAMBOGLOU, Nikolaos ; KUREK, Ralf</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c306t-6bc8464a9ef19645307a9ce3f31393cc05428f5cc3ca58bb09783e89823cf2b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - blood</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Blood</topic><topic>Carcinoma, Papillary - blood</topic><topic>DNA, Complementary - analysis</topic><topic>Endocrinopathies</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Malignant tumors</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Predictive Value of Tests</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Sensitivity and Specificity</topic><topic>Thyroid cancer</topic><topic>Thyroid gland</topic><topic>Thyroid Neoplasms - blood</topic><topic>Thyroid. Thyroid axis (diseases)</topic><topic>Tissue Kallikreins - blood</topic><topic>Tissue Kallikreins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RODDIGER, Sandra J</creatorcontrib><creatorcontrib>RENNEBERG, Heiner</creatorcontrib><creatorcontrib>MARTIN, Thomas</creatorcontrib><creatorcontrib>TUNN, Ulf W</creatorcontrib><creatorcontrib>ZAMBOGLOU, Nikolaos</creatorcontrib><creatorcontrib>KUREK, Ralf</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cancer research and clinical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>RODDIGER, Sandra J</au><au>RENNEBERG, Heiner</au><au>MARTIN, Thomas</au><au>TUNN, Ulf W</au><au>ZAMBOGLOU, Nikolaos</au><au>KUREK, Ralf</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human kallikrein 2 (hk2) mRNA in peripheral blood of patients with thyroid cancer: A novel molecular marker?</atitle><jtitle>Journal of cancer research and clinical oncology</jtitle><addtitle>J Cancer Res Clin Oncol</addtitle><date>2003</date><risdate>2003</risdate><volume>129</volume><issue>1</issue><spage>29</spage><epage>34</epage><pages>29-34</pages><issn>0171-5216</issn><eissn>1432-1335</eissn><coden>JCROD7</coden><abstract>To elucidate whether hK2 mRNA can be detected in peripheral blood of patients with thyroid disease using reverse transcription polymerase chain reaction (RT-PCR).
A nested RT-PCR protocol for the detection of hK2 mRNA was established, and blood samples of 72 patients with a history of thyroid cancer, 10 patients with current metastases of thyroid cancer, and 32 volunteers were tested.
hK2-transcripts were significantly more often detected in patients with thyroid cancer (20/72=28%) than in the control group (2/32=6%, P = 0.03, chi-square analysis).
This is the first study reporting on hK2 as a potential molecular marker for patients with thyroid cancer. We could demonstrate a correlation between diagnosis of thyroid cancer and the positive signal for hK2 in the RT-PCR assay. Future studies are necessary to prove the clinical value of hK2 as a molecular marker regarding recurrence and outcome.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>12618898</pmid><doi>10.1007/s00432-002-0401-y</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0171-5216 |
| ispartof | Journal of cancer research and clinical oncology, 2003, Vol.129 (1), p.29-34 |
| issn | 0171-5216 1432-1335 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Adult Aged Biological and medical sciences Biomarkers, Tumor - blood Biomarkers, Tumor - genetics Blood Carcinoma, Papillary - blood DNA, Complementary - analysis Endocrinopathies Female Humans Male Malignant tumors Medical sciences Middle Aged Predictive Value of Tests Reverse Transcriptase Polymerase Chain Reaction Ribonucleic acid RNA Sensitivity and Specificity Thyroid cancer Thyroid gland Thyroid Neoplasms - blood Thyroid. Thyroid axis (diseases) Tissue Kallikreins - blood Tissue Kallikreins - genetics |
| title | Human kallikrein 2 (hk2) mRNA in peripheral blood of patients with thyroid cancer: A novel molecular marker? |
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