An improved methodology to produce Flavobacterium heparinum chondroitinases, important instruments for diagnosis of diseases

Chondroitinases are very important tools for the identification and structural analysis of proteoglycans. Enzymic analysis with Flavobacterium heparinum chondroitinases has shown that chondroitin sulphate and dermatan sulphate structures are modified in many human diseases, suggesting a diagnostic v...

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Veröffentlicht in:	Biotechnology and applied biochemistry 2003-04, Vol.37 (2), p.115-127
Hauptverfasser:	Aguiar, Jair A. K., Lima, Cilene R., Berto, Alessandra G. A., Michelacci, Yara M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Cell Culture Techniques - methods chondroitin sulphate Chondroitinases and Chondroitin Lyases - biosynthesis Chondroitinases and Chondroitin Lyases - chemistry Chondroitinases and Chondroitin Lyases - classification Chondroitinases and Chondroitin Lyases - isolation & purification Chromatography - methods dermatan sulphate Enzyme Activation Enzyme engineering Enzyme-Linked Immunosorbent Assay - methods Flavobacterium - chemistry Flavobacterium - classification Flavobacterium - enzymology Flavobacterium - growth & development Fundamental and applied biological sciences. Psychology glycosaminoglycan Improved methods for extraction and purification of enzymes Methods. Procedures. Technologies mucopolysaccharidase proteoglycan Quality Control Species Specificity Substrate Specificity
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