Isoenzyme Analysis of Hammondia hammondi and Toxoplasma gondii Sporozoites

Isoenzyme analysis using isoelectrofocusing in polyacrylamide gels was used to distinguish Hammondia hammondi and Toxoplasma gondii sporozoites. Five enzyme systems were studied: aconitase (EC 4.2.1.3), aspartate aminotransferase (EC 2.6.1.1), glucose phosphate isomerase (EC 5.3.1.9), lactate dehydr...

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Veröffentlicht in:	The Journal of parasitology 1992-08, Vol.78 (4), p.731-734
Hauptverfasser:	Dardé, M. L., Riahi, H., Bouteille, B., Pestre-Alexandre, M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aconitate Hydratase - analysis Animals Aspartate Aminotransferases - analysis Biological and medical sciences Cat Diseases - parasitology Cats Coccidia - classification Coccidia - enzymology Coccidia - genetics Coccidiosis - parasitology Coccidiosis - veterinary Cysts Enzymes Fundamental and applied biological sciences. Psychology Glucose-6-Phosphate Isomerase - analysis Hammondia hammondi Infections Invertebrates Isoelectric Focusing Isoenzymes - analysis L-Lactate Dehydrogenase - analysis Mice Oocysts Parasitism Parasitology Phosphoglucomutase - analysis Protozoa Research Notes Sporozoites Systematics. Geographical distribution. Morphology. Cytology Tachyzoites Toxoplasma - classification Toxoplasma - enzymology Toxoplasma - genetics Toxoplasma gondii Toxoplasmosis, Animal - parasitology Veterinary medicine Zymodemes
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Zusammenfassung:	Isoenzyme analysis using isoelectrofocusing in polyacrylamide gels was used to distinguish Hammondia hammondi and Toxoplasma gondii sporozoites. Five enzyme systems were studied: aconitase (EC 4.2.1.3), aspartate aminotransferase (EC 2.6.1.1), glucose phosphate isomerase (EC 5.3.1.9), lactate dehydrogenase (EC 1.1.1.27), and phosphoglucomutase (EC 2.7.5.1). Three stocks of T. gondii belonging to 3 zymodemes were compared to 1 stock of H. hammondi. Hammondia hammondi differed from T. gondii at all 5 loci analyzed. This was observed for all 3 zymodemes of T. gondii. These results indicated clear genetic differences between the 2 species.
ISSN:	0022-3395 1937-2345
DOI:	10.2307/3283552