Transcripts of the viroid central conserved region contain the local tertiary structural element found in full-length viroid
The viroid central conserved region (CCR) is highly conserved among different viroids and is thought to be involved in viroid replication. A novel tertiary structure occurs in the CCR of native circular potato spindle tuber RNAs. To permit more detailed studies of this structural element, a small RN...
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| Veröffentlicht in: | FEBS letters 1992-06, Vol.305 (1), p.9-14 |
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| creator | Paul, C.P. Levine, B.J. Robertson, H.D. Branch, A.D. |
| description | The viroid central conserved region (CCR) is highly conserved among different viroids and is thought to be involved in viroid replication. A novel tertiary structure occurs in the CCR of native circular potato spindle tuber RNAs. To permit more detailed studies of this structural element, a small RNA oligonucleotide containing the CCR of the viroid genome was synthesized. The tertiary structure of these CCR transcripts was examined by UV-crosslinking of the RNA, followed by mapping of the crosslink using limited alkaline digestion and classical RNA secondary analysis. The CCR transcript was found to undergo UV-crosslinking between the same two bases as in full-length viroid, indicating that the tertiary structure is the same and that the CCR transcript will be useful for the affinity purification of host components. |
| doi_str_mv | 10.1016/0014-5793(92)80644-V |
| format | Article |
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A novel tertiary structure occurs in the CCR of native circular potato spindle tuber RNAs. To permit more detailed studies of this structural element, a small RNA oligonucleotide containing the CCR of the viroid genome was synthesized. The tertiary structure of these CCR transcripts was examined by UV-crosslinking of the RNA, followed by mapping of the crosslink using limited alkaline digestion and classical RNA secondary analysis. The CCR transcript was found to undergo UV-crosslinking between the same two bases as in full-length viroid, indicating that the tertiary structure is the same and that the CCR transcript will be useful for the affinity purification of host components.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(92)80644-V</identifier><identifier>PMID: 1633862</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Base Sequence ; Biological and medical sciences ; Electrophoresis, Gel, Two-Dimensional ; Electrophoresis, Polyacrylamide Gel ; Fundamental and applied biological sciences. Psychology ; Microbiology ; Molecular Sequence Data ; Nucleic Acid Conformation ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; RNA structure ; RNA, Viral - chemistry ; RNA, Viral - genetics ; RNA, Viral - radiation effects ; Ultraviolet Rays ; UV-crosslinking ; Viroid ; Viroid-host interaction ; Viroids - genetics ; Viroids - radiation effects ; Viroids - ultrastructure ; Virology</subject><ispartof>FEBS letters, 1992-06, Vol.305 (1), p.9-14</ispartof><rights>1992</rights><rights>FEBS Letters 305 (1992) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c475V-7185ae805d523253f28f5418b50679bed52072017781e25290ca928af1bbf9bb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/001457939280644V$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5371114$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1633862$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Paul, C.P.</creatorcontrib><creatorcontrib>Levine, B.J.</creatorcontrib><creatorcontrib>Robertson, H.D.</creatorcontrib><creatorcontrib>Branch, A.D.</creatorcontrib><title>Transcripts of the viroid central conserved region contain the local tertiary structural element found in full-length viroid</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The viroid central conserved region (CCR) is highly conserved among different viroids and is thought to be involved in viroid replication. A novel tertiary structure occurs in the CCR of native circular potato spindle tuber RNAs. To permit more detailed studies of this structural element, a small RNA oligonucleotide containing the CCR of the viroid genome was synthesized. The tertiary structure of these CCR transcripts was examined by UV-crosslinking of the RNA, followed by mapping of the crosslink using limited alkaline digestion and classical RNA secondary analysis. The CCR transcript was found to undergo UV-crosslinking between the same two bases as in full-length viroid, indicating that the tertiary structure is the same and that the CCR transcript will be useful for the affinity purification of host components.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>RNA structure</subject><subject>RNA, Viral - chemistry</subject><subject>RNA, Viral - genetics</subject><subject>RNA, Viral - radiation effects</subject><subject>Ultraviolet Rays</subject><subject>UV-crosslinking</subject><subject>Viroid</subject><subject>Viroid-host interaction</subject><subject>Viroids - genetics</subject><subject>Viroids - radiation effects</subject><subject>Viroids - ultrastructure</subject><subject>Virology</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9v1DAQxS0EKkvhG4CUA0JwCPhPHDsXJKi6BakSl7JXy3HGrZE3XmxnUSU-PHZ31d6Ak-U3v5mx30PoJcHvCSb9B4xJ13IxsLcDfSdx33Xt5hFaESlYy7pePkare-QpepbSD1zukgwn6IT0jMmertDvq6jnZKLb5dQE2-QbaPYuBjc1BuYctW9MmBPEPUxNhGsX5ipk7eY71gdTkAwxOx1vm5TjYvJS28DDtkxobFjmqSm4XbxvPczX-ea44jl6YrVP8OJ4nqLv6_Orsy_t5beLr2efLlvTCb5pBZFcg8R84pRRziyVlndEjhz3YhihyFhQTISQBCinAzZ6oFJbMo52GEd2it4c5u5i-LlAymrrkgHv9QxhSUowLPqB0H-CpJeSiK4rYHcATQwpRbBqF922OKAIVjUdVa1X1Xo1UHWXjtqUtlfH-cu4hemh6RBHqb8-1nUqvtqSjXHpHuNMEELq9vUB--U83P7XarU-_0xroerFnKrW93w8DILi_t5BVMk4mA1MLoLJagru7x_6A160wE4</recordid><startdate>19920622</startdate><enddate>19920622</enddate><creator>Paul, C.P.</creator><creator>Levine, B.J.</creator><creator>Robertson, H.D.</creator><creator>Branch, A.D.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19920622</creationdate><title>Transcripts of the viroid central conserved region contain the local tertiary structural element found in full-length viroid</title><author>Paul, C.P. ; Levine, B.J. ; Robertson, H.D. ; Branch, A.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475V-7185ae805d523253f28f5418b50679bed52072017781e25290ca928af1bbf9bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>RNA structure</topic><topic>RNA, Viral - chemistry</topic><topic>RNA, Viral - genetics</topic><topic>RNA, Viral - radiation effects</topic><topic>Ultraviolet Rays</topic><topic>UV-crosslinking</topic><topic>Viroid</topic><topic>Viroid-host interaction</topic><topic>Viroids - genetics</topic><topic>Viroids - radiation effects</topic><topic>Viroids - ultrastructure</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Paul, C.P.</creatorcontrib><creatorcontrib>Levine, B.J.</creatorcontrib><creatorcontrib>Robertson, H.D.</creatorcontrib><creatorcontrib>Branch, A.D.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paul, C.P.</au><au>Levine, B.J.</au><au>Robertson, H.D.</au><au>Branch, A.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcripts of the viroid central conserved region contain the local tertiary structural element found in full-length viroid</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1992-06-22</date><risdate>1992</risdate><volume>305</volume><issue>1</issue><spage>9</spage><epage>14</epage><pages>9-14</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>The viroid central conserved region (CCR) is highly conserved among different viroids and is thought to be involved in viroid replication. A novel tertiary structure occurs in the CCR of native circular potato spindle tuber RNAs. To permit more detailed studies of this structural element, a small RNA oligonucleotide containing the CCR of the viroid genome was synthesized. The tertiary structure of these CCR transcripts was examined by UV-crosslinking of the RNA, followed by mapping of the crosslink using limited alkaline digestion and classical RNA secondary analysis. The CCR transcript was found to undergo UV-crosslinking between the same two bases as in full-length viroid, indicating that the tertiary structure is the same and that the CCR transcript will be useful for the affinity purification of host components.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1633862</pmid><doi>10.1016/0014-5793(92)80644-V</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1992-06, Vol.305 (1), p.9-14 |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Base Sequence Biological and medical sciences Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Microbiology Molecular Sequence Data Nucleic Acid Conformation Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains RNA structure RNA, Viral - chemistry RNA, Viral - genetics RNA, Viral - radiation effects Ultraviolet Rays UV-crosslinking Viroid Viroid-host interaction Viroids - genetics Viroids - radiation effects Viroids - ultrastructure Virology |
| title | Transcripts of the viroid central conserved region contain the local tertiary structural element found in full-length viroid |
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