Characterization of Polylactic Acid-Polyglycolic Acid Composites for Cartilage Tissue Engineering
The objective of this study was to determine the effects of scaffold composition on the physical properties, adhesion, and growth of bovine articular chondrocytes on polylactic acid (PLA)/polyglycolic acid (PGA) composites. Nonwoven meshes of PGA were coated with PLA, using a solvent evaporation tec...
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| Veröffentlicht in: | Tissue engineering 2003-02, Vol.9 (1), p.63-70 |
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| creator | Moran, John M. Pazzano, David Bonassar, Lawrence J. |
| description | The objective of this study was to determine the effects of scaffold composition on the physical properties, adhesion, and growth of bovine articular chondrocytes on polylactic acid (PLA)/polyglycolic acid
(PGA) composites. Nonwoven meshes of PGA were coated with PLA, using a solvent evaporation technique that resulted in composites with fractional PLA contents ranging from 0 to 68%. The compressive
modulus of scaffolds increased linearly with the addition of PLA, ranging from less than 1 kPa for PGA to approximately 20 kPa for scaffolds with 68% PLA content. The characteristic degradation time
of these scaffolds also increased from approximately 5 days for 0% PLA to 45 days for 68% PLA. Addition of PLA decreased cell seeding efficiency from 48% for 0% PLA scaffolds
to 27% for 68% PLA scaffolds. Cells seeded onto 27% PLA scaffolds increased 3-fold in number over 4 weeks in culture, whereas cells seeded onto 68% PLA increased only 2-fold
in number. Scanning electron microscopy indicated that cells attached to PGA appeared flat with many small processes, whereas those attached to PLA were more rounded. These studies provide important information
for the design of scaffolds for cartilage tissue engineering. |
| doi_str_mv | 10.1089/107632703762687546 |
| format | Article |
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(PGA) composites. Nonwoven meshes of PGA were coated with PLA, using a solvent evaporation technique that resulted in composites with fractional PLA contents ranging from 0 to 68%. The compressive
modulus of scaffolds increased linearly with the addition of PLA, ranging from less than 1 kPa for PGA to approximately 20 kPa for scaffolds with 68% PLA content. The characteristic degradation time
of these scaffolds also increased from approximately 5 days for 0% PLA to 45 days for 68% PLA. Addition of PLA decreased cell seeding efficiency from 48% for 0% PLA scaffolds
to 27% for 68% PLA scaffolds. Cells seeded onto 27% PLA scaffolds increased 3-fold in number over 4 weeks in culture, whereas cells seeded onto 68% PLA increased only 2-fold
in number. Scanning electron microscopy indicated that cells attached to PGA appeared flat with many small processes, whereas those attached to PLA were more rounded. These studies provide important information
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modulus of scaffolds increased linearly with the addition of PLA, ranging from less than 1 kPa for PGA to approximately 20 kPa for scaffolds with 68% PLA content. The characteristic degradation time
of these scaffolds also increased from approximately 5 days for 0% PLA to 45 days for 68% PLA. Addition of PLA decreased cell seeding efficiency from 48% for 0% PLA scaffolds
to 27% for 68% PLA scaffolds. Cells seeded onto 27% PLA scaffolds increased 3-fold in number over 4 weeks in culture, whereas cells seeded onto 68% PLA increased only 2-fold
in number. Scanning electron microscopy indicated that cells attached to PGA appeared flat with many small processes, whereas those attached to PLA were more rounded. These studies provide important information
for the design of scaffolds for cartilage tissue engineering.</description><subject>Animals</subject><subject>Cartilage - metabolism</subject><subject>Cattle</subject><subject>Cell Adhesion - physiology</subject><subject>Lactic Acid - metabolism</subject><subject>Manufactured Materials</subject><subject>Microscopy, Electron, Scanning</subject><subject>Original Articles</subject><subject>Polyesters</subject><subject>Polyglycolic Acid - metabolism</subject><subject>Polymers - metabolism</subject><subject>Tissue Engineering</subject><issn>1076-3279</issn><issn>1557-8690</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkT9P5DAQxa0Tp-PffQEKFFHQhfPEHjsuUbQcSEhQQB05ib0YeePFTorl0-PVrnTSUUA1M0-_edLMI-QM6BXQWv0BKgWrJGVSVKKWyMUPcgSIsqyFoge5z0CZCXVIjlN6pZQigvxFDqESFSrEI6KbFx11P5no3vXkwlgEWzwGv_FZdH1x3buh3M5Lv-mD3ytFE1brkNxkUmFDLBodJ-f10hRPLqXZFItx6UaTTcflKflptU_m976ekOebxVNzW94__L1rru_LnstqKjvGB865EoJ2SmnLqB3sYBApSuA1oALJajADWl1DHlEO1mqFtbUCeMdOyOXOdx3D22zS1K5c6o33ejRhTq1k-Rsc4EsQ8veYYJjBi__A1zDHMR_RVoACmECeoWoH9TGkFI1t19GtdNy0QNttTO3nmPLS-d557lZm-LeyzyUD9Q7YynocvTOdidN3vD8AVcadxw</recordid><startdate>20030201</startdate><enddate>20030201</enddate><creator>Moran, John M.</creator><creator>Pazzano, David</creator><creator>Bonassar, Lawrence J.</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20030201</creationdate><title>Characterization of Polylactic Acid-Polyglycolic Acid Composites for Cartilage Tissue Engineering</title><author>Moran, John M. ; 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(PGA) composites. Nonwoven meshes of PGA were coated with PLA, using a solvent evaporation technique that resulted in composites with fractional PLA contents ranging from 0 to 68%. The compressive
modulus of scaffolds increased linearly with the addition of PLA, ranging from less than 1 kPa for PGA to approximately 20 kPa for scaffolds with 68% PLA content. The characteristic degradation time
of these scaffolds also increased from approximately 5 days for 0% PLA to 45 days for 68% PLA. Addition of PLA decreased cell seeding efficiency from 48% for 0% PLA scaffolds
to 27% for 68% PLA scaffolds. Cells seeded onto 27% PLA scaffolds increased 3-fold in number over 4 weeks in culture, whereas cells seeded onto 68% PLA increased only 2-fold
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| subjects | Animals Cartilage - metabolism Cattle Cell Adhesion - physiology Lactic Acid - metabolism Manufactured Materials Microscopy, Electron, Scanning Original Articles Polyesters Polyglycolic Acid - metabolism Polymers - metabolism Tissue Engineering |
| title | Characterization of Polylactic Acid-Polyglycolic Acid Composites for Cartilage Tissue Engineering |
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