Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells
We have evaluated the feasibility of large-scale isolation of CD133+ progenitors from healthy mobilized adult donors for potential clinical use in autologous and allogeneic transplantation. A total of 11 healthy volunteer adult donors were mobilized with G-CSF. CD133+ stem cells were isolated from a...
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| Veröffentlicht in: | Bone marrow transplantation (Basingstoke) 2003-01, Vol.31 (1), p.17-22 |
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| container_title | Bone marrow transplantation (Basingstoke) |
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| creator | GORDON, P. R LEIMIG, T BABARIN-DORNER, A HOUSTON, J HOLLADAY, M MUELLER, I GEIGER, T HANDGRETINGER, R |
| description | We have evaluated the feasibility of large-scale isolation of CD133+ progenitors from healthy mobilized adult donors for potential clinical use in autologous and allogeneic transplantation. A total of 11 healthy volunteer adult donors were mobilized with G-CSF. CD133+ stem cells were isolated from a single leukapheresis using the Clinimacs method. The median percentage of CD133 before positive selection was 0.75% (range 0.39-2.03%). After selection, the median purity and recovery was 94% (range 85.2-98.0%) and 69% (range 44-100%), respectively. The median log10 T-cell depletion obtained by CD133+ positive selection was 4.2 (range 3.8-4.7). The CD133+ progenitors were highly enriched in colony-forming units (CFU) and transplantation into NOD/SCID mice resulted in a high engraftment rate. Transplantation of sorted CD133+/CD34+ cells into NOD/SCID mice showed a higher engraftment compared to CD133-/CD34+ cells. Mobilized peripheral CD133+ stem cells can be purified in large scale for potential clinical use. The biological function of the cells is not impaired. The majority of the NOD/SCID repopulating cells are within the CD133+/CD34+ subpopulation. Therefore, clinical studies using purified CD133+ stem cells can be envisoned to further clarify the role of CD133+ stem cells in hematopoietic reconstitution after transplantation. |
| doi_str_mv | 10.1038/sj.bmt.1703792 |
| format | Article |
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R ; LEIMIG, T ; BABARIN-DORNER, A ; HOUSTON, J ; HOLLADAY, M ; MUELLER, I ; GEIGER, T ; HANDGRETINGER, R</creator><creatorcontrib>GORDON, P. R ; LEIMIG, T ; BABARIN-DORNER, A ; HOUSTON, J ; HOLLADAY, M ; MUELLER, I ; GEIGER, T ; HANDGRETINGER, R</creatorcontrib><description>We have evaluated the feasibility of large-scale isolation of CD133+ progenitors from healthy mobilized adult donors for potential clinical use in autologous and allogeneic transplantation. A total of 11 healthy volunteer adult donors were mobilized with G-CSF. CD133+ stem cells were isolated from a single leukapheresis using the Clinimacs method. The median percentage of CD133 before positive selection was 0.75% (range 0.39-2.03%). After selection, the median purity and recovery was 94% (range 85.2-98.0%) and 69% (range 44-100%), respectively. The median log10 T-cell depletion obtained by CD133+ positive selection was 4.2 (range 3.8-4.7). The CD133+ progenitors were highly enriched in colony-forming units (CFU) and transplantation into NOD/SCID mice resulted in a high engraftment rate. Transplantation of sorted CD133+/CD34+ cells into NOD/SCID mice showed a higher engraftment compared to CD133-/CD34+ cells. Mobilized peripheral CD133+ stem cells can be purified in large scale for potential clinical use. The biological function of the cells is not impaired. The majority of the NOD/SCID repopulating cells are within the CD133+/CD34+ subpopulation. Therefore, clinical studies using purified CD133+ stem cells can be envisoned to further clarify the role of CD133+ stem cells in hematopoietic reconstitution after transplantation.</description><identifier>ISSN: 0268-3369</identifier><identifier>EISSN: 1476-5365</identifier><identifier>DOI: 10.1038/sj.bmt.1703792</identifier><identifier>PMID: 12621502</identifier><identifier>CODEN: BMTRE9</identifier><language>eng</language><publisher>Basingstoke: Nature Publishing Group</publisher><subject>AC133 Antigen ; Adult ; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Animals ; Antigens, CD - blood ; Biological and medical sciences ; Bone marrow ; Bone marrow, stem cells transplantation. Graft versus host reaction ; Cell Separation ; Colony-Forming Units Assay ; Complications and side effects ; Development and progression ; DNA Primers ; Filgrastim ; Flow Cytometry ; Glycoproteins - blood ; Graft versus host reaction ; Granulocyte colony-stimulating factor ; Granulocyte Colony-Stimulating Factor - therapeutic use ; Hematopoietic Stem Cell Mobilization - methods ; Hematopoietic stem cells ; Hematopoietic Stem Cells - cytology ; Hematopoietic Stem Cells - immunology ; Humans ; Leukapheresis ; Living Donors ; Medical sciences ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Peptides - blood ; Physiological aspects ; Polymerase Chain Reaction - methods ; Prevention ; Recombinant Proteins ; Stem cell transplantation ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy ; Transplantation ; Transplantation, Heterologous</subject><ispartof>Bone marrow transplantation (Basingstoke), 2003-01, Vol.31 (1), p.17-22</ispartof><rights>2003 INIST-CNRS</rights><rights>COPYRIGHT 2003 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jan 1, 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c577t-293d764fb064198f29ec106266040c34aa2790bdd53207258f9ca8e0684d584f3</citedby><cites>FETCH-LOGICAL-c577t-293d764fb064198f29ec106266040c34aa2790bdd53207258f9ca8e0684d584f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,4012,27912,27913,27914</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14638247$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12621502$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GORDON, P. R</creatorcontrib><creatorcontrib>LEIMIG, T</creatorcontrib><creatorcontrib>BABARIN-DORNER, A</creatorcontrib><creatorcontrib>HOUSTON, J</creatorcontrib><creatorcontrib>HOLLADAY, M</creatorcontrib><creatorcontrib>MUELLER, I</creatorcontrib><creatorcontrib>GEIGER, T</creatorcontrib><creatorcontrib>HANDGRETINGER, R</creatorcontrib><title>Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells</title><title>Bone marrow transplantation (Basingstoke)</title><addtitle>Bone Marrow Transplant</addtitle><description>We have evaluated the feasibility of large-scale isolation of CD133+ progenitors from healthy mobilized adult donors for potential clinical use in autologous and allogeneic transplantation. A total of 11 healthy volunteer adult donors were mobilized with G-CSF. CD133+ stem cells were isolated from a single leukapheresis using the Clinimacs method. The median percentage of CD133 before positive selection was 0.75% (range 0.39-2.03%). After selection, the median purity and recovery was 94% (range 85.2-98.0%) and 69% (range 44-100%), respectively. The median log10 T-cell depletion obtained by CD133+ positive selection was 4.2 (range 3.8-4.7). The CD133+ progenitors were highly enriched in colony-forming units (CFU) and transplantation into NOD/SCID mice resulted in a high engraftment rate. Transplantation of sorted CD133+/CD34+ cells into NOD/SCID mice showed a higher engraftment compared to CD133-/CD34+ cells. Mobilized peripheral CD133+ stem cells can be purified in large scale for potential clinical use. The biological function of the cells is not impaired. The majority of the NOD/SCID repopulating cells are within the CD133+/CD34+ subpopulation. Therefore, clinical studies using purified CD133+ stem cells can be envisoned to further clarify the role of CD133+ stem cells in hematopoietic reconstitution after transplantation.</description><subject>AC133 Antigen</subject><subject>Adult</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Animals</subject><subject>Antigens, CD - blood</subject><subject>Biological and medical sciences</subject><subject>Bone marrow</subject><subject>Bone marrow, stem cells transplantation. Graft versus host reaction</subject><subject>Cell Separation</subject><subject>Colony-Forming Units Assay</subject><subject>Complications and side effects</subject><subject>Development and progression</subject><subject>DNA Primers</subject><subject>Filgrastim</subject><subject>Flow Cytometry</subject><subject>Glycoproteins - blood</subject><subject>Graft versus host reaction</subject><subject>Granulocyte colony-stimulating factor</subject><subject>Granulocyte Colony-Stimulating Factor - therapeutic use</subject><subject>Hematopoietic Stem Cell Mobilization - methods</subject><subject>Hematopoietic stem cells</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Hematopoietic Stem Cells - immunology</subject><subject>Humans</subject><subject>Leukapheresis</subject><subject>Living Donors</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred NOD</subject><subject>Mice, SCID</subject><subject>Peptides - blood</subject><subject>Physiological aspects</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Prevention</subject><subject>Recombinant Proteins</subject><subject>Stem cell transplantation</subject><subject>Transfusions. 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R</au><au>LEIMIG, T</au><au>BABARIN-DORNER, A</au><au>HOUSTON, J</au><au>HOLLADAY, M</au><au>MUELLER, I</au><au>GEIGER, T</au><au>HANDGRETINGER, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells</atitle><jtitle>Bone marrow transplantation (Basingstoke)</jtitle><addtitle>Bone Marrow Transplant</addtitle><date>2003-01-01</date><risdate>2003</risdate><volume>31</volume><issue>1</issue><spage>17</spage><epage>22</epage><pages>17-22</pages><issn>0268-3369</issn><eissn>1476-5365</eissn><coden>BMTRE9</coden><abstract>We have evaluated the feasibility of large-scale isolation of CD133+ progenitors from healthy mobilized adult donors for potential clinical use in autologous and allogeneic transplantation. A total of 11 healthy volunteer adult donors were mobilized with G-CSF. CD133+ stem cells were isolated from a single leukapheresis using the Clinimacs method. The median percentage of CD133 before positive selection was 0.75% (range 0.39-2.03%). After selection, the median purity and recovery was 94% (range 85.2-98.0%) and 69% (range 44-100%), respectively. The median log10 T-cell depletion obtained by CD133+ positive selection was 4.2 (range 3.8-4.7). The CD133+ progenitors were highly enriched in colony-forming units (CFU) and transplantation into NOD/SCID mice resulted in a high engraftment rate. Transplantation of sorted CD133+/CD34+ cells into NOD/SCID mice showed a higher engraftment compared to CD133-/CD34+ cells. Mobilized peripheral CD133+ stem cells can be purified in large scale for potential clinical use. The biological function of the cells is not impaired. The majority of the NOD/SCID repopulating cells are within the CD133+/CD34+ subpopulation. Therefore, clinical studies using purified CD133+ stem cells can be envisoned to further clarify the role of CD133+ stem cells in hematopoietic reconstitution after transplantation.</abstract><cop>Basingstoke</cop><pub>Nature Publishing Group</pub><pmid>12621502</pmid><doi>10.1038/sj.bmt.1703792</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0268-3369 |
| ispartof | Bone marrow transplantation (Basingstoke), 2003-01, Vol.31 (1), p.17-22 |
| issn | 0268-3369 1476-5365 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Nature Journals Online; SpringerLink Journals - AutoHoldings |
| subjects | AC133 Antigen Adult Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Antigens, CD - blood Biological and medical sciences Bone marrow Bone marrow, stem cells transplantation. Graft versus host reaction Cell Separation Colony-Forming Units Assay Complications and side effects Development and progression DNA Primers Filgrastim Flow Cytometry Glycoproteins - blood Graft versus host reaction Granulocyte colony-stimulating factor Granulocyte Colony-Stimulating Factor - therapeutic use Hematopoietic Stem Cell Mobilization - methods Hematopoietic stem cells Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - immunology Humans Leukapheresis Living Donors Medical sciences Mice Mice, Inbred NOD Mice, SCID Peptides - blood Physiological aspects Polymerase Chain Reaction - methods Prevention Recombinant Proteins Stem cell transplantation Transfusions. Complications. Transfusion reactions. Cell and gene therapy Transplantation Transplantation, Heterologous |
| title | Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells |
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