Nongenomic Effects of Aldosterone on Human Renal Cells
The development of chronic renal insufficiency may be partially mediated by the nongenomic action of aldosterone. Here we investigate whether aldosterone could evoke a nongenomic action in primary cultures of human renal cells. Intracellular Ca2+ ([Ca2+]i) and cAMP were measured in human mesangial c...
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| Veröffentlicht in: | The journal of clinical endocrinology and metabolism 2003-03, Vol.88 (3), p.1297-1302 |
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| container_title | The journal of clinical endocrinology and metabolism |
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| creator | Köppel, H. Christ, M. Yard, B. A. Bär, P. C. van der Woude, F. J. Wehling, M. |
| description | The development of chronic renal insufficiency may be partially mediated by the nongenomic action of aldosterone. Here we investigate whether aldosterone could evoke a nongenomic action in primary cultures of human renal cells. Intracellular Ca2+ ([Ca2+]i) and cAMP were measured in human mesangial cells (MC), glomerular visceral epithelial cells (GVEC), and proximal and distal tubular epithelial cells (Ptec and Dtec) in the presence of aldosterone (10–100 nmol/liter) by fura-2 fluorescence and RIA, respectively.
In MC, Ptec, and Dtec, aldosterone increased [Ca2+]i within 1 min, whereas in GVEC, only a minor effect was found. Preincubation of cells with spironolactone did not blunt this effect. Hydrocortisone, used at a concentration 100-fold higher than that of aldosterone, did not affect [Ca2+]i. In MC, Ptec, and Dtec, a dose-dependent increase (∼1.3- to 1.5-fold) in intracellular cAMP levels was found.
These data demonstrate a nongenomic action of aldosterone in human MC, Ptec, and Dtec. As these effects occur at concentrations close to free plasma aldosterone levels in man, they may be of physiological relevance and may contribute to renal injury. |
| doi_str_mv | 10.1210/jc.2002-020248 |
| format | Article |
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In MC, Ptec, and Dtec, aldosterone increased [Ca2+]i within 1 min, whereas in GVEC, only a minor effect was found. Preincubation of cells with spironolactone did not blunt this effect. Hydrocortisone, used at a concentration 100-fold higher than that of aldosterone, did not affect [Ca2+]i. In MC, Ptec, and Dtec, a dose-dependent increase (∼1.3- to 1.5-fold) in intracellular cAMP levels was found.
These data demonstrate a nongenomic action of aldosterone in human MC, Ptec, and Dtec. As these effects occur at concentrations close to free plasma aldosterone levels in man, they may be of physiological relevance and may contribute to renal injury.</description><identifier>ISSN: 0021-972X</identifier><identifier>EISSN: 1945-7197</identifier><identifier>DOI: 10.1210/jc.2002-020248</identifier><identifier>PMID: 12629122</identifier><identifier>CODEN: JCEMAZ</identifier><language>eng</language><publisher>Bethesda, MD: Endocrine Society</publisher><subject>Aldosterone - pharmacology ; Biological and medical sciences ; Calcium - metabolism ; Cells, Cultured ; Cyclic AMP - biosynthesis ; Dose-Response Relationship, Drug ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; Glomerular Mesangium - drug effects ; Glomerular Mesangium - metabolism ; Hormones. Endocrine system ; Humans ; Kidney - cytology ; Kidney - drug effects ; Kidney - metabolism ; Kidney Tubules, Distal - drug effects ; Kidney Tubules, Distal - metabolism ; Kidney Tubules, Proximal - drug effects ; Kidney Tubules, Proximal - metabolism ; Medical sciences ; Pharmacology. Drug treatments</subject><ispartof>The journal of clinical endocrinology and metabolism, 2003-03, Vol.88 (3), p.1297-1302</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-f89cc38f6f2fbce250a785ab36c49f7a64753d7bf59e3ef21b2fe1820b17a9673</citedby><cites>FETCH-LOGICAL-c473t-f89cc38f6f2fbce250a785ab36c49f7a64753d7bf59e3ef21b2fe1820b17a9673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14591539$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12629122$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Köppel, H.</creatorcontrib><creatorcontrib>Christ, M.</creatorcontrib><creatorcontrib>Yard, B. A.</creatorcontrib><creatorcontrib>Bär, P. C.</creatorcontrib><creatorcontrib>van der Woude, F. J.</creatorcontrib><creatorcontrib>Wehling, M.</creatorcontrib><title>Nongenomic Effects of Aldosterone on Human Renal Cells</title><title>The journal of clinical endocrinology and metabolism</title><addtitle>J Clin Endocrinol Metab</addtitle><description>The development of chronic renal insufficiency may be partially mediated by the nongenomic action of aldosterone. Here we investigate whether aldosterone could evoke a nongenomic action in primary cultures of human renal cells. Intracellular Ca2+ ([Ca2+]i) and cAMP were measured in human mesangial cells (MC), glomerular visceral epithelial cells (GVEC), and proximal and distal tubular epithelial cells (Ptec and Dtec) in the presence of aldosterone (10–100 nmol/liter) by fura-2 fluorescence and RIA, respectively.
In MC, Ptec, and Dtec, aldosterone increased [Ca2+]i within 1 min, whereas in GVEC, only a minor effect was found. Preincubation of cells with spironolactone did not blunt this effect. Hydrocortisone, used at a concentration 100-fold higher than that of aldosterone, did not affect [Ca2+]i. In MC, Ptec, and Dtec, a dose-dependent increase (∼1.3- to 1.5-fold) in intracellular cAMP levels was found.
These data demonstrate a nongenomic action of aldosterone in human MC, Ptec, and Dtec. As these effects occur at concentrations close to free plasma aldosterone levels in man, they may be of physiological relevance and may contribute to renal injury.</description><subject>Aldosterone - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>Cells, Cultured</subject><subject>Cyclic AMP - biosynthesis</subject><subject>Dose-Response Relationship, Drug</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - metabolism</subject><subject>Glomerular Mesangium - drug effects</subject><subject>Glomerular Mesangium - metabolism</subject><subject>Hormones. Endocrine system</subject><subject>Humans</subject><subject>Kidney - cytology</subject><subject>Kidney - drug effects</subject><subject>Kidney - metabolism</subject><subject>Kidney Tubules, Distal - drug effects</subject><subject>Kidney Tubules, Distal - metabolism</subject><subject>Kidney Tubules, Proximal - drug effects</subject><subject>Kidney Tubules, Proximal - metabolism</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><issn>0021-972X</issn><issn>1945-7197</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10M9LwzAUwPEgipvTq0fpRW-d-dE0zXGM6YShIAreQpq-SEubzGQ9-N_b0cJOnnLI5z0eX4RuCV4SSvBjY5YUY5piimlWnKE5kRlPBZHiHM2HD5JKQb9m6CrGBmOSZZxdohmhOZWE0jnKX737Bue72iQba8EcYuJtsmorHw8QvIPEu2Tbd9ol7-B0m6yhbeM1urC6jXAzvQv0-bT5WG_T3dvzy3q1S00m2CG1hTSGFTa31JYGKMdaFFyXLDeZtELnmeCsEqXlEhhYSkpqgRQUl0RomQu2QA_j3n3wPz3Eg-rqaIYLtAPfRyUYFpQQPMDlCE3wMQawah_qTodfRbA6llKNUcdSaiw1DNxNm_uyg-rEpzQDuJ-Ajka3Nmhn6nhyGZeEMzk4PjpwlTehdrAPEKNqfB-GXvG_A_4AmgKA9Q</recordid><startdate>20030301</startdate><enddate>20030301</enddate><creator>Köppel, H.</creator><creator>Christ, M.</creator><creator>Yard, B. A.</creator><creator>Bär, P. C.</creator><creator>van der Woude, F. J.</creator><creator>Wehling, M.</creator><general>Endocrine Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030301</creationdate><title>Nongenomic Effects of Aldosterone on Human Renal Cells</title><author>Köppel, H. ; Christ, M. ; Yard, B. A. ; Bär, P. C. ; van der Woude, F. J. ; Wehling, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-f89cc38f6f2fbce250a785ab36c49f7a64753d7bf59e3ef21b2fe1820b17a9673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Aldosterone - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>Cells, Cultured</topic><topic>Cyclic AMP - biosynthesis</topic><topic>Dose-Response Relationship, Drug</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - metabolism</topic><topic>Glomerular Mesangium - drug effects</topic><topic>Glomerular Mesangium - metabolism</topic><topic>Hormones. Endocrine system</topic><topic>Humans</topic><topic>Kidney - cytology</topic><topic>Kidney - drug effects</topic><topic>Kidney - metabolism</topic><topic>Kidney Tubules, Distal - drug effects</topic><topic>Kidney Tubules, Distal - metabolism</topic><topic>Kidney Tubules, Proximal - drug effects</topic><topic>Kidney Tubules, Proximal - metabolism</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Köppel, H.</creatorcontrib><creatorcontrib>Christ, M.</creatorcontrib><creatorcontrib>Yard, B. A.</creatorcontrib><creatorcontrib>Bär, P. C.</creatorcontrib><creatorcontrib>van der Woude, F. J.</creatorcontrib><creatorcontrib>Wehling, M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of clinical endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Köppel, H.</au><au>Christ, M.</au><au>Yard, B. A.</au><au>Bär, P. C.</au><au>van der Woude, F. J.</au><au>Wehling, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nongenomic Effects of Aldosterone on Human Renal Cells</atitle><jtitle>The journal of clinical endocrinology and metabolism</jtitle><addtitle>J Clin Endocrinol Metab</addtitle><date>2003-03-01</date><risdate>2003</risdate><volume>88</volume><issue>3</issue><spage>1297</spage><epage>1302</epage><pages>1297-1302</pages><issn>0021-972X</issn><eissn>1945-7197</eissn><coden>JCEMAZ</coden><abstract>The development of chronic renal insufficiency may be partially mediated by the nongenomic action of aldosterone. Here we investigate whether aldosterone could evoke a nongenomic action in primary cultures of human renal cells. Intracellular Ca2+ ([Ca2+]i) and cAMP were measured in human mesangial cells (MC), glomerular visceral epithelial cells (GVEC), and proximal and distal tubular epithelial cells (Ptec and Dtec) in the presence of aldosterone (10–100 nmol/liter) by fura-2 fluorescence and RIA, respectively.
In MC, Ptec, and Dtec, aldosterone increased [Ca2+]i within 1 min, whereas in GVEC, only a minor effect was found. Preincubation of cells with spironolactone did not blunt this effect. Hydrocortisone, used at a concentration 100-fold higher than that of aldosterone, did not affect [Ca2+]i. In MC, Ptec, and Dtec, a dose-dependent increase (∼1.3- to 1.5-fold) in intracellular cAMP levels was found.
These data demonstrate a nongenomic action of aldosterone in human MC, Ptec, and Dtec. As these effects occur at concentrations close to free plasma aldosterone levels in man, they may be of physiological relevance and may contribute to renal injury.</abstract><cop>Bethesda, MD</cop><pub>Endocrine Society</pub><pmid>12629122</pmid><doi>10.1210/jc.2002-020248</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Aldosterone - pharmacology Biological and medical sciences Calcium - metabolism Cells, Cultured Cyclic AMP - biosynthesis Dose-Response Relationship, Drug Epithelial Cells - drug effects Epithelial Cells - metabolism Glomerular Mesangium - drug effects Glomerular Mesangium - metabolism Hormones. Endocrine system Humans Kidney - cytology Kidney - drug effects Kidney - metabolism Kidney Tubules, Distal - drug effects Kidney Tubules, Distal - metabolism Kidney Tubules, Proximal - drug effects Kidney Tubules, Proximal - metabolism Medical sciences Pharmacology. Drug treatments |
| title | Nongenomic Effects of Aldosterone on Human Renal Cells |
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