A Mak‐like kinase is a repressor of GAMYB in barley aleurone
Summary GAMYB is a gibberellin (GA)‐regulated activator of hydrolase gene expression in the aleurone layer of germinating cereal grains. Although it is clear that GAMYB expression is regulated by GA, more remains to be understood about how this transcription factor operates within the GA‐response pa...
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| Veröffentlicht in: | The Plant journal : for cell and molecular biology 2003-02, Vol.33 (4), p.707-717 |
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| container_title | The Plant journal : for cell and molecular biology |
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| creator | Woodger, Fiona J. Gubler, Frank Pogson, Barry J. Jacobsen, John V. |
| description | Summary
GAMYB is a gibberellin (GA)‐regulated activator of hydrolase gene expression in the aleurone layer of germinating cereal grains. Although it is clear that GAMYB expression is regulated by GA, more remains to be understood about how this transcription factor operates within the GA‐response pathway. In order to isolate new components from the GA‐response pathway, barley aleurone libraries were screened for GAMYB‐binding proteins using a recently developed yeast two‐hybrid system, which is compatible with the use of transcription factors as baits. We isolated a new member of the emerging Mak‐subgroup of cdc2‐ and MAP kinase‐related protein kinases. We have termed this GAMYB‐binding protein KGM (for kinase associated with GAMYB). Transient expression of KGM specifically repressed α‐amylase promoter activity at the level of GAMYB function but a mutation designed to de‐stabilise the activation loop of KGM alleviated this repression. We propose that KGM is a negative regulator of GAMYB function in aleurone that may prevent precocious hydrolase gene expression. |
| doi_str_mv | 10.1046/j.1365-313X.2003.01663.x |
| format | Article |
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GAMYB is a gibberellin (GA)‐regulated activator of hydrolase gene expression in the aleurone layer of germinating cereal grains. Although it is clear that GAMYB expression is regulated by GA, more remains to be understood about how this transcription factor operates within the GA‐response pathway. In order to isolate new components from the GA‐response pathway, barley aleurone libraries were screened for GAMYB‐binding proteins using a recently developed yeast two‐hybrid system, which is compatible with the use of transcription factors as baits. We isolated a new member of the emerging Mak‐subgroup of cdc2‐ and MAP kinase‐related protein kinases. We have termed this GAMYB‐binding protein KGM (for kinase associated with GAMYB). Transient expression of KGM specifically repressed α‐amylase promoter activity at the level of GAMYB function but a mutation designed to de‐stabilise the activation loop of KGM alleviated this repression. We propose that KGM is a negative regulator of GAMYB function in aleurone that may prevent precocious hydrolase gene expression.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1046/j.1365-313X.2003.01663.x</identifier><identifier>PMID: 12609043</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>aleurone ; alpha-Amylases - metabolism ; Amino Acid Sequence ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; GAMYB ; Gene Expression Regulation, Enzymologic - drug effects ; Gene Expression Regulation, Plant - drug effects ; Germination - genetics ; Germination - physiology ; Germination and dormancy ; gibberellin ; Gibberellins - pharmacology ; Hordeum - enzymology ; Hordeum - genetics ; Hordeum - growth & development ; Mak ; Mitogen-Activated Protein Kinases - genetics ; Mitogen-Activated Protein Kinases - metabolism ; Molecular Sequence Data ; Mutation ; Plant physiology and development ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Proto-Oncogene Proteins c-myb - genetics ; Proto-Oncogene Proteins c-myb - metabolism ; Repressor Proteins - genetics ; Repressor Proteins - metabolism ; Seeds - enzymology ; Seeds - genetics ; Seeds - growth & development ; Sequence Homology, Amino Acid ; Signal Transduction - genetics ; Signal Transduction - physiology ; signalling ; Two-Hybrid System Techniques ; α‐amylase</subject><ispartof>The Plant journal : for cell and molecular biology, 2003-02, Vol.33 (4), p.707-717</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4923-b7d20be373a0f5c9fcb73f887adc4008589725a6c633932e8eb18ae4fe6fd9923</citedby><cites>FETCH-LOGICAL-c4923-b7d20be373a0f5c9fcb73f887adc4008589725a6c633932e8eb18ae4fe6fd9923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-313X.2003.01663.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-313X.2003.01663.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14579176$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12609043$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Woodger, Fiona J.</creatorcontrib><creatorcontrib>Gubler, Frank</creatorcontrib><creatorcontrib>Pogson, Barry J.</creatorcontrib><creatorcontrib>Jacobsen, John V.</creatorcontrib><title>A Mak‐like kinase is a repressor of GAMYB in barley aleurone</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>Summary
GAMYB is a gibberellin (GA)‐regulated activator of hydrolase gene expression in the aleurone layer of germinating cereal grains. Although it is clear that GAMYB expression is regulated by GA, more remains to be understood about how this transcription factor operates within the GA‐response pathway. In order to isolate new components from the GA‐response pathway, barley aleurone libraries were screened for GAMYB‐binding proteins using a recently developed yeast two‐hybrid system, which is compatible with the use of transcription factors as baits. We isolated a new member of the emerging Mak‐subgroup of cdc2‐ and MAP kinase‐related protein kinases. We have termed this GAMYB‐binding protein KGM (for kinase associated with GAMYB). Transient expression of KGM specifically repressed α‐amylase promoter activity at the level of GAMYB function but a mutation designed to de‐stabilise the activation loop of KGM alleviated this repression. We propose that KGM is a negative regulator of GAMYB function in aleurone that may prevent precocious hydrolase gene expression.</description><subject>aleurone</subject><subject>alpha-Amylases - metabolism</subject><subject>Amino Acid Sequence</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GAMYB</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Germination - genetics</subject><subject>Germination - physiology</subject><subject>Germination and dormancy</subject><subject>gibberellin</subject><subject>Gibberellins - pharmacology</subject><subject>Hordeum - enzymology</subject><subject>Hordeum - genetics</subject><subject>Hordeum - growth & development</subject><subject>Mak</subject><subject>Mitogen-Activated Protein Kinases - genetics</subject><subject>Mitogen-Activated Protein Kinases - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Plant physiology and development</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Proto-Oncogene Proteins c-myb - genetics</subject><subject>Proto-Oncogene Proteins c-myb - metabolism</subject><subject>Repressor Proteins - genetics</subject><subject>Repressor Proteins - metabolism</subject><subject>Seeds - enzymology</subject><subject>Seeds - genetics</subject><subject>Seeds - growth & development</subject><subject>Sequence Homology, Amino Acid</subject><subject>Signal Transduction - genetics</subject><subject>Signal Transduction - physiology</subject><subject>signalling</subject><subject>Two-Hybrid System Techniques</subject><subject>α‐amylase</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM9O20AQh1eoFYTAK6C90JvdWa-9fw4ghahQqqD2EKT2tFpvZiUnjh12azXc-gh9xj4JNongCKcZab7fzOgjhDJIGeTi8zJlXBQJZ_xnmgHwFJgQPN0ekNHL4AMZgRaQyJxlR-Q4xiUAk1zkh-SIZQI05HxELif0zq7-__1XVyukq6qxEWkVqaUBNwFjbANtPb2Z3P26olVDSxtqfKS2xi60DZ6Qj97WEU_3dUzur7_Mp1-T2feb2-lklrhcZzwp5SKDErnkFnzhtHel5F4paRcuB1CF0jIrrHCCc80zVFgyZTH3KPxC9xvG5NNu7ya0Dx3G32ZdRYd1bRtsu2gkB8m0ehtkSoKUvYcxUTvQhTbGgN5sQrW24dEwMINkszSDSzO4NINk8yzZbPvo2f5GV65x8RrcW-2B8z1go7O1D7ZxVXzl8kJqJkXPXey4P1Uv9d0PmPmPb0PHnwBfgJbQ</recordid><startdate>200302</startdate><enddate>200302</enddate><creator>Woodger, Fiona J.</creator><creator>Gubler, Frank</creator><creator>Pogson, Barry J.</creator><creator>Jacobsen, John V.</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200302</creationdate><title>A Mak‐like kinase is a repressor of GAMYB in barley aleurone</title><author>Woodger, Fiona J. ; Gubler, Frank ; Pogson, Barry J. ; Jacobsen, John V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4923-b7d20be373a0f5c9fcb73f887adc4008589725a6c633932e8eb18ae4fe6fd9923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>aleurone</topic><topic>alpha-Amylases - metabolism</topic><topic>Amino Acid Sequence</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GAMYB</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Germination - genetics</topic><topic>Germination - physiology</topic><topic>Germination and dormancy</topic><topic>gibberellin</topic><topic>Gibberellins - pharmacology</topic><topic>Hordeum - enzymology</topic><topic>Hordeum - genetics</topic><topic>Hordeum - growth & development</topic><topic>Mak</topic><topic>Mitogen-Activated Protein Kinases - genetics</topic><topic>Mitogen-Activated Protein Kinases - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Plant physiology and development</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Proto-Oncogene Proteins c-myb - genetics</topic><topic>Proto-Oncogene Proteins c-myb - metabolism</topic><topic>Repressor Proteins - genetics</topic><topic>Repressor Proteins - metabolism</topic><topic>Seeds - enzymology</topic><topic>Seeds - genetics</topic><topic>Seeds - growth & development</topic><topic>Sequence Homology, Amino Acid</topic><topic>Signal Transduction - genetics</topic><topic>Signal Transduction - physiology</topic><topic>signalling</topic><topic>Two-Hybrid System Techniques</topic><topic>α‐amylase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Woodger, Fiona J.</creatorcontrib><creatorcontrib>Gubler, Frank</creatorcontrib><creatorcontrib>Pogson, Barry J.</creatorcontrib><creatorcontrib>Jacobsen, John V.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Woodger, Fiona J.</au><au>Gubler, Frank</au><au>Pogson, Barry J.</au><au>Jacobsen, John V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Mak‐like kinase is a repressor of GAMYB in barley aleurone</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2003-02</date><risdate>2003</risdate><volume>33</volume><issue>4</issue><spage>707</spage><epage>717</epage><pages>707-717</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>Summary
GAMYB is a gibberellin (GA)‐regulated activator of hydrolase gene expression in the aleurone layer of germinating cereal grains. Although it is clear that GAMYB expression is regulated by GA, more remains to be understood about how this transcription factor operates within the GA‐response pathway. In order to isolate new components from the GA‐response pathway, barley aleurone libraries were screened for GAMYB‐binding proteins using a recently developed yeast two‐hybrid system, which is compatible with the use of transcription factors as baits. We isolated a new member of the emerging Mak‐subgroup of cdc2‐ and MAP kinase‐related protein kinases. We have termed this GAMYB‐binding protein KGM (for kinase associated with GAMYB). Transient expression of KGM specifically repressed α‐amylase promoter activity at the level of GAMYB function but a mutation designed to de‐stabilise the activation loop of KGM alleviated this repression. We propose that KGM is a negative regulator of GAMYB function in aleurone that may prevent precocious hydrolase gene expression.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>12609043</pmid><doi>10.1046/j.1365-313X.2003.01663.x</doi><tpages>11</tpages></addata></record> |
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| ispartof | The Plant journal : for cell and molecular biology, 2003-02, Vol.33 (4), p.707-717 |
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| subjects | aleurone alpha-Amylases - metabolism Amino Acid Sequence Biological and medical sciences Fundamental and applied biological sciences. Psychology GAMYB Gene Expression Regulation, Enzymologic - drug effects Gene Expression Regulation, Plant - drug effects Germination - genetics Germination - physiology Germination and dormancy gibberellin Gibberellins - pharmacology Hordeum - enzymology Hordeum - genetics Hordeum - growth & development Mak Mitogen-Activated Protein Kinases - genetics Mitogen-Activated Protein Kinases - metabolism Molecular Sequence Data Mutation Plant physiology and development Plant Proteins - genetics Plant Proteins - metabolism Proto-Oncogene Proteins c-myb - genetics Proto-Oncogene Proteins c-myb - metabolism Repressor Proteins - genetics Repressor Proteins - metabolism Seeds - enzymology Seeds - genetics Seeds - growth & development Sequence Homology, Amino Acid Signal Transduction - genetics Signal Transduction - physiology signalling Two-Hybrid System Techniques α‐amylase |
| title | A Mak‐like kinase is a repressor of GAMYB in barley aleurone |
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