Optimum conditions for the turkey lymphocyte transformation test

Optimum conditions for turkey lymphocyte transformation tests were determined. Thrice-washed turkey buffy-coat cells obtained after slow centrifugation (40 X g, 10 minutes) responded well to mitogenic stimulation. Turkey lymphocytes isolated on Ficoll-containing separation media largely lost their a...

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Veröffentlicht in:	Avian diseases 1992-04, Vol.36 (2), p.386-394
Hauptverfasser:	Barta, O. (Virginia Polytechnic Institute and State University, Blacksburg, VA), Barta, V, Domermuth, C.H, Pierson, F.W
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Blood Cells, Cultured Centrifugation, Density Gradient Cultured cells DINDON Immune Sera - immunology Leukocyte Count - veterinary Lymphocyte Activation Lymphocytes Lymphocytes - immunology Mitogens Mitogens - administration & dosage PAVO Pokeweed mitogens T tests TECHNIQUE IMMUNOLOGIQUE TECNICAS INMUNOLOGICAS Temperature Turkeys Turkeys - blood Turkeys - immunology Ungulates Washing
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