Gene synthesis and expression in E. coli for pump, a human matrix metalloproteinase

The gene for PUMP ( putative metalloproteinase), a human matrix metalloproteinase, was synthesized by a PCR-based method. The DNA fragment of 546 bases containing the PUMP gene was generated by overlap extension of six long oligonucleotides (length ranging from 101 to 116 bases) and subsequent ampli...

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Veröffentlicht in:	Biochemical and biophysical research communications 1992-07, Vol.186 (1), p.143-149
Hauptverfasser:	Ye, Qi-Zhuang, Johnson, Linda L., Baragi, Vijaykumar
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical, structural and metabolic biochemistry Biological and medical sciences Cloning, Molecular - methods DNA - genetics DNA - isolation & purification Enzymes and enzyme inhibitors Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Expression Genes, Synthetic Humans Hydrolases Matrix Metalloproteinase 7 Metalloendopeptidases - genetics Metalloendopeptidases - isolation & purification Molecular Sequence Data Molecular Weight Oligodeoxyribonucleotides Plasmids Polymerase Chain Reaction - methods Recombinant Proteins - isolation & purification Restriction Mapping
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