Efferent vagal fibre stimulation blunts nuclear factor-kappaB activation and protects against hypovolemic hemorrhagic shock
We investigated whether electrical stimulation (STIM) of efferent vagus nerves may suppress nuclear factor (NF)-kappaB activation and the inflammatory cascade in hemorrhagic (Hem) shock. Rats were subjected to bilateral cervical vagotomy (VGX) or sham surgical procedures. Hem shock was induced by in...
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| Veröffentlicht in: | Circulation (New York, N.Y.) N.Y.), 2003-03, Vol.107 (8), p.1189-1194 |
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| creator | Guarini, Salvatore Altavilla, Domenica Cainazzo, Maria-Michela Giuliani, Daniela Bigiani, Albertino Marini, Herbert Squadrito, Giovanni Minutoli, Letteria Bertolini, Alfio Marini, Rolando Adamo, Elena B Venuti, Francesco S Squadrito, Francesco |
| description | We investigated whether electrical stimulation (STIM) of efferent vagus nerves may suppress nuclear factor (NF)-kappaB activation and the inflammatory cascade in hemorrhagic (Hem) shock.
Rats were subjected to bilateral cervical vagotomy (VGX) or sham surgical procedures. Hem shock was induced by intermittent withdrawing of blood until mean arterial pressure stabilized within the range of 35 to 40 mm Hg. Application of constant voltage pulses to the caudal vagus ends (STIM; 5 V, 2 ms, 1 Hz for 12 minutes, 5 minutes after mean arterial pressure stabilization) increased survival time (VGX+Hem+Sham STIM=38+/-3 minutes; VGX+Hem+STIM >180 minutes), reverted the marked hypotension (VGX+Hem+Sham STIM=33+/-3 mm Hg; VGX+Hem+STIM=66+/-5 mm Hg), inhibited IkappaBalpha liver loss, and blunted the augmented NF-kappaB activity, decreased hepatic tumor necrosis factor (TNF)-alpha mRNA (VGX+Hem+Sham STIM=1.42+/-0.5 amount of TNF-alpha m-RNA; VGX+Hem+STIM=0.51+/-0.2 amount of TNF-alpha mRNA), and reduced plasma TNF-alpha (VGX+Hem+Sham STIM=190+/-24 pg/mL; VGX+Hem+STIM=87+/-15 pg/mL). Chlorisondamine, a nicotinic receptor antagonist, abated the effects of vagal stimulation.
Our results show a parasympathetic inhibition of NF-kappaB by which the brain opposes NF-kappaB activation in the liver and modulates the inflammatory response during acute hypovolemic hemorrhagic shock. |
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Rats were subjected to bilateral cervical vagotomy (VGX) or sham surgical procedures. Hem shock was induced by intermittent withdrawing of blood until mean arterial pressure stabilized within the range of 35 to 40 mm Hg. Application of constant voltage pulses to the caudal vagus ends (STIM; 5 V, 2 ms, 1 Hz for 12 minutes, 5 minutes after mean arterial pressure stabilization) increased survival time (VGX+Hem+Sham STIM=38+/-3 minutes; VGX+Hem+STIM >180 minutes), reverted the marked hypotension (VGX+Hem+Sham STIM=33+/-3 mm Hg; VGX+Hem+STIM=66+/-5 mm Hg), inhibited IkappaBalpha liver loss, and blunted the augmented NF-kappaB activity, decreased hepatic tumor necrosis factor (TNF)-alpha mRNA (VGX+Hem+Sham STIM=1.42+/-0.5 amount of TNF-alpha m-RNA; VGX+Hem+STIM=0.51+/-0.2 amount of TNF-alpha mRNA), and reduced plasma TNF-alpha (VGX+Hem+Sham STIM=190+/-24 pg/mL; VGX+Hem+STIM=87+/-15 pg/mL). Chlorisondamine, a nicotinic receptor antagonist, abated the effects of vagal stimulation.
Our results show a parasympathetic inhibition of NF-kappaB by which the brain opposes NF-kappaB activation in the liver and modulates the inflammatory response during acute hypovolemic hemorrhagic shock.</description><identifier>ISSN: 0009-7322</identifier><identifier>EISSN: 1524-4539</identifier><identifier>PMID: 12615800</identifier><identifier>CODEN: CIRCAZ</identifier><language>eng</language><publisher>United States: American Heart Association, Inc</publisher><subject>Animals ; Blood Pressure ; Efferent Pathways ; Electric Stimulation ; Hypovolemia - immunology ; Hypovolemia - metabolism ; Hypovolemia - physiopathology ; I-kappa B Proteins - metabolism ; Inflammation - genetics ; Inflammation - metabolism ; Inflammation - prevention & control ; Liver - innervation ; Liver - metabolism ; Male ; Nerve Fibers - physiology ; NF-kappa B - antagonists & inhibitors ; NF-KappaB Inhibitor alpha ; Rats ; Rats, Wistar ; RNA, Messenger - metabolism ; Shock, Hemorrhagic - immunology ; Shock, Hemorrhagic - metabolism ; Shock, Hemorrhagic - prevention & control ; Survival Analysis ; Tumor Necrosis Factor-alpha - analysis ; Tumor Necrosis Factor-alpha - biosynthesis ; Tumor Necrosis Factor-alpha - genetics ; Vagus Nerve - physiology</subject><ispartof>Circulation (New York, N.Y.), 2003-03, Vol.107 (8), p.1189-1194</ispartof><rights>Copyright American Heart Association, Inc. Mar 4 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12615800$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guarini, Salvatore</creatorcontrib><creatorcontrib>Altavilla, Domenica</creatorcontrib><creatorcontrib>Cainazzo, Maria-Michela</creatorcontrib><creatorcontrib>Giuliani, Daniela</creatorcontrib><creatorcontrib>Bigiani, Albertino</creatorcontrib><creatorcontrib>Marini, Herbert</creatorcontrib><creatorcontrib>Squadrito, Giovanni</creatorcontrib><creatorcontrib>Minutoli, Letteria</creatorcontrib><creatorcontrib>Bertolini, Alfio</creatorcontrib><creatorcontrib>Marini, Rolando</creatorcontrib><creatorcontrib>Adamo, Elena B</creatorcontrib><creatorcontrib>Venuti, Francesco S</creatorcontrib><creatorcontrib>Squadrito, Francesco</creatorcontrib><title>Efferent vagal fibre stimulation blunts nuclear factor-kappaB activation and protects against hypovolemic hemorrhagic shock</title><title>Circulation (New York, N.Y.)</title><addtitle>Circulation</addtitle><description>We investigated whether electrical stimulation (STIM) of efferent vagus nerves may suppress nuclear factor (NF)-kappaB activation and the inflammatory cascade in hemorrhagic (Hem) shock.
Rats were subjected to bilateral cervical vagotomy (VGX) or sham surgical procedures. Hem shock was induced by intermittent withdrawing of blood until mean arterial pressure stabilized within the range of 35 to 40 mm Hg. Application of constant voltage pulses to the caudal vagus ends (STIM; 5 V, 2 ms, 1 Hz for 12 minutes, 5 minutes after mean arterial pressure stabilization) increased survival time (VGX+Hem+Sham STIM=38+/-3 minutes; VGX+Hem+STIM >180 minutes), reverted the marked hypotension (VGX+Hem+Sham STIM=33+/-3 mm Hg; VGX+Hem+STIM=66+/-5 mm Hg), inhibited IkappaBalpha liver loss, and blunted the augmented NF-kappaB activity, decreased hepatic tumor necrosis factor (TNF)-alpha mRNA (VGX+Hem+Sham STIM=1.42+/-0.5 amount of TNF-alpha m-RNA; VGX+Hem+STIM=0.51+/-0.2 amount of TNF-alpha mRNA), and reduced plasma TNF-alpha (VGX+Hem+Sham STIM=190+/-24 pg/mL; VGX+Hem+STIM=87+/-15 pg/mL). Chlorisondamine, a nicotinic receptor antagonist, abated the effects of vagal stimulation.
Our results show a parasympathetic inhibition of NF-kappaB by which the brain opposes NF-kappaB activation in the liver and modulates the inflammatory response during acute hypovolemic hemorrhagic shock.</description><subject>Animals</subject><subject>Blood Pressure</subject><subject>Efferent Pathways</subject><subject>Electric Stimulation</subject><subject>Hypovolemia - immunology</subject><subject>Hypovolemia - metabolism</subject><subject>Hypovolemia - physiopathology</subject><subject>I-kappa B Proteins - metabolism</subject><subject>Inflammation - genetics</subject><subject>Inflammation - metabolism</subject><subject>Inflammation - prevention & control</subject><subject>Liver - innervation</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Nerve Fibers - physiology</subject><subject>NF-kappa B - antagonists & inhibitors</subject><subject>NF-KappaB Inhibitor alpha</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RNA, Messenger - metabolism</subject><subject>Shock, Hemorrhagic - immunology</subject><subject>Shock, Hemorrhagic - metabolism</subject><subject>Shock, Hemorrhagic - prevention & control</subject><subject>Survival Analysis</subject><subject>Tumor Necrosis Factor-alpha - analysis</subject><subject>Tumor Necrosis Factor-alpha - biosynthesis</subject><subject>Tumor Necrosis Factor-alpha - genetics</subject><subject>Vagus Nerve - physiology</subject><issn>0009-7322</issn><issn>1524-4539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0M9LwzAUB_AiipvTf0GCB2-FNGn70qMOf8HAy-4lyV7Xbm1Sk3Qw_OeNbF48vfeFD18e7yKZZwXL07zg1WUyp5RWKXDGZsmN97sYSw7FdTLLWJkVgtJ58v3SNOjQBHKQW9mTplMOiQ_dMPUydNYQ1U8meGIm3aN0pJE6WJfu5TjKZxJDdzg5aTZkdDagjjp2dcYH0h5He7A9Dp0mLQ7WuVZu4-5bq_e3yVUje49357lI1q8v6-V7uvp8-1g-rdJRZDTVTUVLFIzDpqoAoJRKYYWgJKWFyJhoQOVMVKUWCDmDSnIKBVVKQYmgkS-Sx1NtvO5rQh_qofMa-14atJOvgdMyFyAifPgHd3ZyJp5Ws4wB5yX7RfdnNKkBN_XoukG6Y_33Uv4DxX11bA</recordid><startdate>20030304</startdate><enddate>20030304</enddate><creator>Guarini, Salvatore</creator><creator>Altavilla, Domenica</creator><creator>Cainazzo, Maria-Michela</creator><creator>Giuliani, Daniela</creator><creator>Bigiani, Albertino</creator><creator>Marini, Herbert</creator><creator>Squadrito, Giovanni</creator><creator>Minutoli, Letteria</creator><creator>Bertolini, Alfio</creator><creator>Marini, Rolando</creator><creator>Adamo, Elena B</creator><creator>Venuti, Francesco S</creator><creator>Squadrito, Francesco</creator><general>American Heart Association, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>U9A</scope><scope>7X8</scope></search><sort><creationdate>20030304</creationdate><title>Efferent vagal fibre stimulation blunts nuclear factor-kappaB activation and protects against hypovolemic hemorrhagic shock</title><author>Guarini, Salvatore ; Altavilla, Domenica ; Cainazzo, Maria-Michela ; Giuliani, Daniela ; Bigiani, Albertino ; Marini, Herbert ; Squadrito, Giovanni ; Minutoli, Letteria ; Bertolini, Alfio ; Marini, Rolando ; Adamo, Elena B ; Venuti, Francesco S ; Squadrito, Francesco</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p810-cf906e8237d997776abbe9e7ba0058128f7b42896c8e74279a30750bbb76e7ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Blood Pressure</topic><topic>Efferent Pathways</topic><topic>Electric Stimulation</topic><topic>Hypovolemia - immunology</topic><topic>Hypovolemia - metabolism</topic><topic>Hypovolemia - physiopathology</topic><topic>I-kappa B Proteins - metabolism</topic><topic>Inflammation - genetics</topic><topic>Inflammation - metabolism</topic><topic>Inflammation - prevention & control</topic><topic>Liver - innervation</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Nerve Fibers - physiology</topic><topic>NF-kappa B - antagonists & inhibitors</topic><topic>NF-KappaB Inhibitor alpha</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>RNA, Messenger - metabolism</topic><topic>Shock, Hemorrhagic - immunology</topic><topic>Shock, Hemorrhagic - metabolism</topic><topic>Shock, Hemorrhagic - prevention & control</topic><topic>Survival Analysis</topic><topic>Tumor Necrosis Factor-alpha - analysis</topic><topic>Tumor Necrosis Factor-alpha - biosynthesis</topic><topic>Tumor Necrosis Factor-alpha - genetics</topic><topic>Vagus Nerve - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guarini, Salvatore</creatorcontrib><creatorcontrib>Altavilla, Domenica</creatorcontrib><creatorcontrib>Cainazzo, Maria-Michela</creatorcontrib><creatorcontrib>Giuliani, Daniela</creatorcontrib><creatorcontrib>Bigiani, Albertino</creatorcontrib><creatorcontrib>Marini, Herbert</creatorcontrib><creatorcontrib>Squadrito, Giovanni</creatorcontrib><creatorcontrib>Minutoli, Letteria</creatorcontrib><creatorcontrib>Bertolini, Alfio</creatorcontrib><creatorcontrib>Marini, Rolando</creatorcontrib><creatorcontrib>Adamo, Elena B</creatorcontrib><creatorcontrib>Venuti, Francesco S</creatorcontrib><creatorcontrib>Squadrito, Francesco</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Circulation (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guarini, Salvatore</au><au>Altavilla, Domenica</au><au>Cainazzo, Maria-Michela</au><au>Giuliani, Daniela</au><au>Bigiani, Albertino</au><au>Marini, Herbert</au><au>Squadrito, Giovanni</au><au>Minutoli, Letteria</au><au>Bertolini, Alfio</au><au>Marini, Rolando</au><au>Adamo, Elena B</au><au>Venuti, Francesco S</au><au>Squadrito, Francesco</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efferent vagal fibre stimulation blunts nuclear factor-kappaB activation and protects against hypovolemic hemorrhagic shock</atitle><jtitle>Circulation (New York, N.Y.)</jtitle><addtitle>Circulation</addtitle><date>2003-03-04</date><risdate>2003</risdate><volume>107</volume><issue>8</issue><spage>1189</spage><epage>1194</epage><pages>1189-1194</pages><issn>0009-7322</issn><eissn>1524-4539</eissn><coden>CIRCAZ</coden><abstract>We investigated whether electrical stimulation (STIM) of efferent vagus nerves may suppress nuclear factor (NF)-kappaB activation and the inflammatory cascade in hemorrhagic (Hem) shock.
Rats were subjected to bilateral cervical vagotomy (VGX) or sham surgical procedures. Hem shock was induced by intermittent withdrawing of blood until mean arterial pressure stabilized within the range of 35 to 40 mm Hg. Application of constant voltage pulses to the caudal vagus ends (STIM; 5 V, 2 ms, 1 Hz for 12 minutes, 5 minutes after mean arterial pressure stabilization) increased survival time (VGX+Hem+Sham STIM=38+/-3 minutes; VGX+Hem+STIM >180 minutes), reverted the marked hypotension (VGX+Hem+Sham STIM=33+/-3 mm Hg; VGX+Hem+STIM=66+/-5 mm Hg), inhibited IkappaBalpha liver loss, and blunted the augmented NF-kappaB activity, decreased hepatic tumor necrosis factor (TNF)-alpha mRNA (VGX+Hem+Sham STIM=1.42+/-0.5 amount of TNF-alpha m-RNA; VGX+Hem+STIM=0.51+/-0.2 amount of TNF-alpha mRNA), and reduced plasma TNF-alpha (VGX+Hem+Sham STIM=190+/-24 pg/mL; VGX+Hem+STIM=87+/-15 pg/mL). Chlorisondamine, a nicotinic receptor antagonist, abated the effects of vagal stimulation.
Our results show a parasympathetic inhibition of NF-kappaB by which the brain opposes NF-kappaB activation in the liver and modulates the inflammatory response during acute hypovolemic hemorrhagic shock.</abstract><cop>United States</cop><pub>American Heart Association, Inc</pub><pmid>12615800</pmid><tpages>6</tpages></addata></record> |
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| subjects | Animals Blood Pressure Efferent Pathways Electric Stimulation Hypovolemia - immunology Hypovolemia - metabolism Hypovolemia - physiopathology I-kappa B Proteins - metabolism Inflammation - genetics Inflammation - metabolism Inflammation - prevention & control Liver - innervation Liver - metabolism Male Nerve Fibers - physiology NF-kappa B - antagonists & inhibitors NF-KappaB Inhibitor alpha Rats Rats, Wistar RNA, Messenger - metabolism Shock, Hemorrhagic - immunology Shock, Hemorrhagic - metabolism Shock, Hemorrhagic - prevention & control Survival Analysis Tumor Necrosis Factor-alpha - analysis Tumor Necrosis Factor-alpha - biosynthesis Tumor Necrosis Factor-alpha - genetics Vagus Nerve - physiology |
| title | Efferent vagal fibre stimulation blunts nuclear factor-kappaB activation and protects against hypovolemic hemorrhagic shock |
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