Tracking of cell surface receptors by fluorescence digital imaging microscopy using a charge-coupled device camera. Low-density lipoprotein and influenza virus receptor mobility at 4 degrees C

A fluorescence imaging system, based on using a cooled slow-scan CCD camera, has been developed for tracking receptors on the surfaces of living cells. The technique is applicable to receptors for particles such as lipoproteins and viruses that can be labeled with a few tens of fluorophores. The pos...

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Veröffentlicht in:	Journal of cell science 1992-02, Vol.101 ( Pt 2), p.415-425
Hauptverfasser:	Anderson, C M, Georgiou, G N, Morrison, I E, Stevenson, G V, Cherry, R J
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological Transport Cell Membrane - metabolism Cell Membrane - ultrastructure Cold Temperature Diffusion Fibroblasts - ultrastructure Humans Image Processing, Computer-Assisted - methods Microscopy, Fluorescence - methods Models, Statistical Orthomyxoviridae Receptors, LDL - metabolism Receptors, LDL - ultrastructure Receptors, Virus - metabolism Receptors, Virus - ultrastructure
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