The effects of cooling mouse oocytes

The effects of cooling and warming on meiotic spindles of mouse oocytes have been assessed by transmission electron microscopy. Intact cumulus-oocyte complexes were immediately cooled from 37 to 15, 4, 0, and -7 degrees C (seeding temperature) for 15 min in a programmed biological freezer and fixed...

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Veröffentlicht in:	Journal of assisted reproduction and genetics 1992-04, Vol.9 (2), p.139-148
Hauptverfasser:	Sathananthan, A H, Kirby, C, Trounson, A, Philipatos, D, Shaw, J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chromosome Aberrations Cold Temperature Cryopreservation Freezing Humans Meiosis Mice Mice, Inbred C57BL Mice, Inbred CBA Microscopy, Electron Microtubules - ultrastructure Oocytes - ultrastructure Organelles - ultrastructure Single-Blind Method Species Specificity Spindle Apparatus - ultrastructure
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