Suppression of gene expression in plant cells utilizing antisense sequences transcribed by RNA polymerase III

Inverted sequences of the chloramphenicol acetyltransferase (CAT) reporter gene were fused to a soybean tRNA(met(i)) gene lacking a terminator such that the tRNA(met(i)) sequences caused the co-transcription of CAT antisense sequences by RNA polymerase III. When electroporated into carrot protoplast...

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Veröffentlicht in:	Plant molecular biology 1992-07, Vol.19 (4), p.641-647
Hauptverfasser:	Bourque, J.E. (Missouri Univ., Columbia, MO (USA). Dept. of Biochemistry), Folk, W.R
Format:	Artikel
Sprache:	eng
Schlagworte:	ARN Biological and medical sciences Cell Line CHLORAMPHENICOL Chloramphenicol O-Acetyltransferase - genetics Chloramphenicol O-Acetyltransferase - metabolism CLORAMFENICOL DAUCUS CAROTA EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE EXPRESSION Gene Expression - drug effects GLYCINE MAX Humans Molecular and cellular biology Molecular genetics NUCLEOTIDE SEQUENCE Plants - drug effects Plants - genetics Plasmids Protoplasts RNA RNA Polymerase III - metabolism RNA, Antisense - biosynthesis RNA, Antisense - pharmacology RNA, Transfer, Met - genetics SECUENCIA NUCLEICA SEQUENCE NUCLEIQUE Transcription, Genetic
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