Peripheral blood lymphocytes immunophenotype and serum concentration of soluble HLA class I in burn patients
The level of the soluble form of histocompatibility class I antigens, associated with β 2-microglobulin (sHLA-I) has been determined by an ELISA sandwich method in serum from burned patients ( n=42) and healthy volunteers ( n=30). The sHLA-I level was insignificantly increased in burn patients at th...
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| Veröffentlicht in: | Burns 2003-03, Vol.29 (2), p.123-128 |
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| creator | Lebedev, M.Ju Krizhanova, M.A Vilkov, S.A Sholkina, M.N Vyasmina, E.S Baryshnikov, A.Ju Novikov, V.V |
| description | The level of the soluble form of histocompatibility class I antigens, associated with β
2-microglobulin (sHLA-I) has been determined by an ELISA sandwich method in serum from burned patients (
n=42) and healthy volunteers (
n=30). The sHLA-I level was insignificantly increased in burn patients at the stage of burn shock (1284±324
U/ml, mean±S.E.M.) and after day 28 postburn (1368±258
U/ml) compared to volunteers (1150±90
U/ml). At the same time a decrease of sHLA-I levels between 4 and 14 days (638±178
U/ml) was determined (
P70% in comparison to patients with TBSAB from 30 to 70% during burn shock (1493±528 and 1075±339
U/ml, respectively). Expression of membranous HLA class I antigens (mHLA-I) in peripheral blood lymphocytes (PBLs) was assayed simultaneously by indirect immunofluorescence. The number of CD3
+, CD4
+, CD8
+, CD25
+, CD71
+ and CD26
+ lymphocytes was also evaluated. The expression of mHLA-I in PBLs was increased significantly in patients with TBSAB |
| doi_str_mv | 10.1016/S0305-4179(02)00245-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73059093</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0305417902002450</els_id><sourcerecordid>73059093</sourcerecordid><originalsourceid>FETCH-LOGICAL-c361t-3db828d55ac466095c2f2da8410a6650a9ae3fc473cd2abde6f3d1cb1975c4303</originalsourceid><addsrcrecordid>eNqFkE1r3DAQhkVJaDZpfkKLTiE9OB1Zlj9OJYQ0CSwk0OYsZGnMqsiSK9mB_ffRftAeO5c5zDPzMg8hnxncMGD1t5_AQRQVa7prKL8ClJUo4ANZsbbpClZBd0JWf5Ezcp7Sb8glWvhIzlhZM1GJZkXcC0Y7bTAqR3sXgqFuO06boLczJmrHcfEhj32YtxNS5Q1NGJeR6uA1-jmq2QZPw0BTcEvvkD6ub6l2KiX6RK2n_RI9nTKV4fSJnA7KJbw89gvy-uP-191jsX5-eLq7XRea12wuuOnbsjVCKF3VNXRCl0NpVFsxUHUtQHUK-aCrhmtTqt5gPXDDdM-6RuiKA78gV4e7Uwx_FkyzHG3S6JzyGJYkm-ylg45nUBxAHUNKEQc5RTuquJUM5E6z3GuWO4cSSrnXLHcBX44BSz-i-bd19JqB7wcA85tvFqNMOivQaGxEPUsT7H8i3gFH8o5z</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73059093</pqid></control><display><type>article</type><title>Peripheral blood lymphocytes immunophenotype and serum concentration of soluble HLA class I in burn patients</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Lebedev, M.Ju ; Krizhanova, M.A ; Vilkov, S.A ; Sholkina, M.N ; Vyasmina, E.S ; Baryshnikov, A.Ju ; Novikov, V.V</creator><creatorcontrib>Lebedev, M.Ju ; Krizhanova, M.A ; Vilkov, S.A ; Sholkina, M.N ; Vyasmina, E.S ; Baryshnikov, A.Ju ; Novikov, V.V</creatorcontrib><description>The level of the soluble form of histocompatibility class I antigens, associated with β
2-microglobulin (sHLA-I) has been determined by an ELISA sandwich method in serum from burned patients (
n=42) and healthy volunteers (
n=30). The sHLA-I level was insignificantly increased in burn patients at the stage of burn shock (1284±324
U/ml, mean±S.E.M.) and after day 28 postburn (1368±258
U/ml) compared to volunteers (1150±90
U/ml). At the same time a decrease of sHLA-I levels between 4 and 14 days (638±178
U/ml) was determined (
P<0.05). Increased levels of sHLA, though not significant, were detected in patients with TBSAB >70% in comparison to patients with TBSAB from 30 to 70% during burn shock (1493±528 and 1075±339
U/ml, respectively). Expression of membranous HLA class I antigens (mHLA-I) in peripheral blood lymphocytes (PBLs) was assayed simultaneously by indirect immunofluorescence. The number of CD3
+, CD4
+, CD8
+, CD25
+, CD71
+ and CD26
+ lymphocytes was also evaluated. The expression of mHLA-I in PBLs was increased significantly in patients with TBSAB <70% at early postburn period. Daily monitoring showed that the relative numbers of CD25
+ and CD71
+ lymphocytes in patients varied greatly within short intervals of time during burn shock. The data obtained suggest that mHLA-I expression can reflect postburn lymphocyte activation. The serum content of sHLA-I does not depend on lymphocyte number or activated lymphocyte number in peripheral blood at burned patients.</description><identifier>ISSN: 0305-4179</identifier><identifier>EISSN: 1879-1409</identifier><identifier>DOI: 10.1016/S0305-4179(02)00245-0</identifier><identifier>PMID: 12615457</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Activation antigens ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antigens, CD - blood ; beta 2-Microglobulin - blood ; Burn ; Burns - blood ; Burns - immunology ; Enzyme-Linked Immunosorbent Assay ; Female ; Histocompatibility Antigens Class I - blood ; Histocompatibility Antigens Class I - immunology ; Humans ; Immunophenotyping ; Lymphocyte ; Lymphocytes - immunology ; Male ; mHLA class I ; Middle Aged ; Receptors, Antigen - blood ; sHLA class I ; T-Lymphocyte Subsets - immunology</subject><ispartof>Burns, 2003-03, Vol.29 (2), p.123-128</ispartof><rights>2003 Elsevier Science Ltd and ISBI</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-3db828d55ac466095c2f2da8410a6650a9ae3fc473cd2abde6f3d1cb1975c4303</citedby><cites>FETCH-LOGICAL-c361t-3db828d55ac466095c2f2da8410a6650a9ae3fc473cd2abde6f3d1cb1975c4303</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0305417902002450$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12615457$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lebedev, M.Ju</creatorcontrib><creatorcontrib>Krizhanova, M.A</creatorcontrib><creatorcontrib>Vilkov, S.A</creatorcontrib><creatorcontrib>Sholkina, M.N</creatorcontrib><creatorcontrib>Vyasmina, E.S</creatorcontrib><creatorcontrib>Baryshnikov, A.Ju</creatorcontrib><creatorcontrib>Novikov, V.V</creatorcontrib><title>Peripheral blood lymphocytes immunophenotype and serum concentration of soluble HLA class I in burn patients</title><title>Burns</title><addtitle>Burns</addtitle><description>The level of the soluble form of histocompatibility class I antigens, associated with β
2-microglobulin (sHLA-I) has been determined by an ELISA sandwich method in serum from burned patients (
n=42) and healthy volunteers (
n=30). The sHLA-I level was insignificantly increased in burn patients at the stage of burn shock (1284±324
U/ml, mean±S.E.M.) and after day 28 postburn (1368±258
U/ml) compared to volunteers (1150±90
U/ml). At the same time a decrease of sHLA-I levels between 4 and 14 days (638±178
U/ml) was determined (
P<0.05). Increased levels of sHLA, though not significant, were detected in patients with TBSAB >70% in comparison to patients with TBSAB from 30 to 70% during burn shock (1493±528 and 1075±339
U/ml, respectively). Expression of membranous HLA class I antigens (mHLA-I) in peripheral blood lymphocytes (PBLs) was assayed simultaneously by indirect immunofluorescence. The number of CD3
+, CD4
+, CD8
+, CD25
+, CD71
+ and CD26
+ lymphocytes was also evaluated. The expression of mHLA-I in PBLs was increased significantly in patients with TBSAB <70% at early postburn period. Daily monitoring showed that the relative numbers of CD25
+ and CD71
+ lymphocytes in patients varied greatly within short intervals of time during burn shock. The data obtained suggest that mHLA-I expression can reflect postburn lymphocyte activation. The serum content of sHLA-I does not depend on lymphocyte number or activated lymphocyte number in peripheral blood at burned patients.</description><subject>Activation antigens</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antigens, CD - blood</subject><subject>beta 2-Microglobulin - blood</subject><subject>Burn</subject><subject>Burns - blood</subject><subject>Burns - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Histocompatibility Antigens Class I - blood</subject><subject>Histocompatibility Antigens Class I - immunology</subject><subject>Humans</subject><subject>Immunophenotyping</subject><subject>Lymphocyte</subject><subject>Lymphocytes - immunology</subject><subject>Male</subject><subject>mHLA class I</subject><subject>Middle Aged</subject><subject>Receptors, Antigen - blood</subject><subject>sHLA class I</subject><subject>T-Lymphocyte Subsets - immunology</subject><issn>0305-4179</issn><issn>1879-1409</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVJaDZpfkKLTiE9OB1Zlj9OJYQ0CSwk0OYsZGnMqsiSK9mB_ffRftAeO5c5zDPzMg8hnxncMGD1t5_AQRQVa7prKL8ClJUo4ANZsbbpClZBd0JWf5Ezcp7Sb8glWvhIzlhZM1GJZkXcC0Y7bTAqR3sXgqFuO06boLczJmrHcfEhj32YtxNS5Q1NGJeR6uA1-jmq2QZPw0BTcEvvkD6ub6l2KiX6RK2n_RI9nTKV4fSJnA7KJbw89gvy-uP-191jsX5-eLq7XRea12wuuOnbsjVCKF3VNXRCl0NpVFsxUHUtQHUK-aCrhmtTqt5gPXDDdM-6RuiKA78gV4e7Uwx_FkyzHG3S6JzyGJYkm-ylg45nUBxAHUNKEQc5RTuquJUM5E6z3GuWO4cSSrnXLHcBX44BSz-i-bd19JqB7wcA85tvFqNMOivQaGxEPUsT7H8i3gFH8o5z</recordid><startdate>20030301</startdate><enddate>20030301</enddate><creator>Lebedev, M.Ju</creator><creator>Krizhanova, M.A</creator><creator>Vilkov, S.A</creator><creator>Sholkina, M.N</creator><creator>Vyasmina, E.S</creator><creator>Baryshnikov, A.Ju</creator><creator>Novikov, V.V</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030301</creationdate><title>Peripheral blood lymphocytes immunophenotype and serum concentration of soluble HLA class I in burn patients</title><author>Lebedev, M.Ju ; Krizhanova, M.A ; Vilkov, S.A ; Sholkina, M.N ; Vyasmina, E.S ; Baryshnikov, A.Ju ; Novikov, V.V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-3db828d55ac466095c2f2da8410a6650a9ae3fc473cd2abde6f3d1cb1975c4303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Activation antigens</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Antigens, CD - blood</topic><topic>beta 2-Microglobulin - blood</topic><topic>Burn</topic><topic>Burns - blood</topic><topic>Burns - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Histocompatibility Antigens Class I - blood</topic><topic>Histocompatibility Antigens Class I - immunology</topic><topic>Humans</topic><topic>Immunophenotyping</topic><topic>Lymphocyte</topic><topic>Lymphocytes - immunology</topic><topic>Male</topic><topic>mHLA class I</topic><topic>Middle Aged</topic><topic>Receptors, Antigen - blood</topic><topic>sHLA class I</topic><topic>T-Lymphocyte Subsets - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lebedev, M.Ju</creatorcontrib><creatorcontrib>Krizhanova, M.A</creatorcontrib><creatorcontrib>Vilkov, S.A</creatorcontrib><creatorcontrib>Sholkina, M.N</creatorcontrib><creatorcontrib>Vyasmina, E.S</creatorcontrib><creatorcontrib>Baryshnikov, A.Ju</creatorcontrib><creatorcontrib>Novikov, V.V</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Burns</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lebedev, M.Ju</au><au>Krizhanova, M.A</au><au>Vilkov, S.A</au><au>Sholkina, M.N</au><au>Vyasmina, E.S</au><au>Baryshnikov, A.Ju</au><au>Novikov, V.V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Peripheral blood lymphocytes immunophenotype and serum concentration of soluble HLA class I in burn patients</atitle><jtitle>Burns</jtitle><addtitle>Burns</addtitle><date>2003-03-01</date><risdate>2003</risdate><volume>29</volume><issue>2</issue><spage>123</spage><epage>128</epage><pages>123-128</pages><issn>0305-4179</issn><eissn>1879-1409</eissn><abstract>The level of the soluble form of histocompatibility class I antigens, associated with β
2-microglobulin (sHLA-I) has been determined by an ELISA sandwich method in serum from burned patients (
n=42) and healthy volunteers (
n=30). The sHLA-I level was insignificantly increased in burn patients at the stage of burn shock (1284±324
U/ml, mean±S.E.M.) and after day 28 postburn (1368±258
U/ml) compared to volunteers (1150±90
U/ml). At the same time a decrease of sHLA-I levels between 4 and 14 days (638±178
U/ml) was determined (
P<0.05). Increased levels of sHLA, though not significant, were detected in patients with TBSAB >70% in comparison to patients with TBSAB from 30 to 70% during burn shock (1493±528 and 1075±339
U/ml, respectively). Expression of membranous HLA class I antigens (mHLA-I) in peripheral blood lymphocytes (PBLs) was assayed simultaneously by indirect immunofluorescence. The number of CD3
+, CD4
+, CD8
+, CD25
+, CD71
+ and CD26
+ lymphocytes was also evaluated. The expression of mHLA-I in PBLs was increased significantly in patients with TBSAB <70% at early postburn period. Daily monitoring showed that the relative numbers of CD25
+ and CD71
+ lymphocytes in patients varied greatly within short intervals of time during burn shock. The data obtained suggest that mHLA-I expression can reflect postburn lymphocyte activation. The serum content of sHLA-I does not depend on lymphocyte number or activated lymphocyte number in peripheral blood at burned patients.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>12615457</pmid><doi>10.1016/S0305-4179(02)00245-0</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-4179 |
| ispartof | Burns, 2003-03, Vol.29 (2), p.123-128 |
| issn | 0305-4179 1879-1409 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73059093 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Activation antigens Adolescent Adult Aged Aged, 80 and over Antigens, CD - blood beta 2-Microglobulin - blood Burn Burns - blood Burns - immunology Enzyme-Linked Immunosorbent Assay Female Histocompatibility Antigens Class I - blood Histocompatibility Antigens Class I - immunology Humans Immunophenotyping Lymphocyte Lymphocytes - immunology Male mHLA class I Middle Aged Receptors, Antigen - blood sHLA class I T-Lymphocyte Subsets - immunology |
| title | Peripheral blood lymphocytes immunophenotype and serum concentration of soluble HLA class I in burn patients |
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