Amplification of P450c21 expression in cultured mammalian cells
We describe in this paper an investigation of mammalian expression systems for P450c21 (21-hydroxylase). Four different promoters, the SV40 early and late promoters, MMTV-LTR, and CMV immediate early promoter were tested for their ability to drive the expression of P450c21 in cultured COS-1 cells. W...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1992-07, Vol.186 (1), p.426-431 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Ricketts, Michael H. Chiao, Eric Hu, Meng-Chun Chung, Bon-chu |
| description | We describe in this paper an investigation of mammalian expression systems for P450c21 (21-hydroxylase). Four different promoters, the SV40 early and late promoters, MMTV-LTR, and CMV immediate early promoter were tested for their ability to drive the expression of P450c21 in cultured COS-1 cells. With the exception of MMTV-LTR, all drove the expression of similar levels of functional 21-hydroxylase. In addition, the Rat-1 cell line was tested and shown to be suitable for the stable expression of functional P450c21. We have established cell lines derived from Rat-1 either normal or mutant P450c21 stably expressed together with amplifiable markers. The expression of P450c21 was further increased by selection in methotrexate. |
| doi_str_mv | 10.1016/S0006-291X(05)80825-3 |
| format | Article |
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Four different promoters, the SV40 early and late promoters, MMTV-LTR, and CMV immediate early promoter were tested for their ability to drive the expression of P450c21 in cultured COS-1 cells. With the exception of MMTV-LTR, all drove the expression of similar levels of functional 21-hydroxylase. In addition, the Rat-1 cell line was tested and shown to be suitable for the stable expression of functional P450c21. We have established cell lines derived from Rat-1 either normal or mutant P450c21 stably expressed together with amplifiable markers. The expression of P450c21 was further increased by selection in methotrexate.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/S0006-291X(05)80825-3</identifier><identifier>PMID: 1321611</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Cell Line ; Clone Cells ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; Gene Amplification ; Genes, Regulator ; Kinetics ; Mammary Tumor Virus, Mouse - genetics ; Oxidoreductases ; Plasmids ; Promoter Regions, Genetic ; Repetitive Sequences, Nucleic Acid ; Simian virus 40 - genetics ; Steroid 21-Hydroxylase - genetics ; Steroid 21-Hydroxylase - isolation & purification ; Steroid 21-Hydroxylase - metabolism ; Transfection</subject><ispartof>Biochemical and biophysical research communications, 1992-07, Vol.186 (1), p.426-431</ispartof><rights>1992 Academic Press, Inc.</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-db1588953b8b2847df7d8c9d624e77b4e41f99f72da0053d86d7a0aa31c52e793</citedby><cites>FETCH-LOGICAL-c389t-db1588953b8b2847df7d8c9d624e77b4e41f99f72da0053d86d7a0aa31c52e793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X05808253$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5463296$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1321611$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ricketts, Michael H.</creatorcontrib><creatorcontrib>Chiao, Eric</creatorcontrib><creatorcontrib>Hu, Meng-Chun</creatorcontrib><creatorcontrib>Chung, Bon-chu</creatorcontrib><title>Amplification of P450c21 expression in cultured mammalian cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>We describe in this paper an investigation of mammalian expression systems for P450c21 (21-hydroxylase). Four different promoters, the SV40 early and late promoters, MMTV-LTR, and CMV immediate early promoter were tested for their ability to drive the expression of P450c21 in cultured COS-1 cells. With the exception of MMTV-LTR, all drove the expression of similar levels of functional 21-hydroxylase. In addition, the Rat-1 cell line was tested and shown to be suitable for the stable expression of functional P450c21. We have established cell lines derived from Rat-1 either normal or mutant P450c21 stably expressed together with amplifiable markers. The expression of P450c21 was further increased by selection in methotrexate.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Clone Cells</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Amplification</subject><subject>Genes, Regulator</subject><subject>Kinetics</subject><subject>Mammary Tumor Virus, Mouse - genetics</subject><subject>Oxidoreductases</subject><subject>Plasmids</subject><subject>Promoter Regions, Genetic</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Simian virus 40 - genetics</subject><subject>Steroid 21-Hydroxylase - genetics</subject><subject>Steroid 21-Hydroxylase - isolation & purification</subject><subject>Steroid 21-Hydroxylase - metabolism</subject><subject>Transfection</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVI2G7S_ISADyWkB6czkmVLp7CENA0EEmgDuQlZGoOKP7aSXdp_H3t3SY89Dcw8M_PyMHaBcI2A5ZfvAFDmXOPrFcjPChSXuThiawQNOUcojtn6HfnATlP6CYBYlHrFVig4lohrdrPptm1ogrNjGPpsaLLnQoLjmNGfbaSUlm7oMze14xTJZ53tOtsGO7eobdNHdtLYNtH5oZ6xl693P26_5Y9P9w-3m8fcCaXH3NcoldJS1Krmqqh8U3nltC95QVVVF1Rgo3VTcW8BpPCq9JUFawU6yanS4oxd7u9u4_BrojSaLqQlge1pmJKpBEgFWsyg3IMuDilFasw2hs7GvwbBLOLMTpxZrBiQZifOLHsXhwdT3ZH_t7U3Nc8_HeY2Ods20fYupHdMFqXgupyxmz1Gs4zfgaJJLlDvyIdIbjR-CP8J8gbGIIio</recordid><startdate>19920715</startdate><enddate>19920715</enddate><creator>Ricketts, Michael H.</creator><creator>Chiao, Eric</creator><creator>Hu, Meng-Chun</creator><creator>Chung, Bon-chu</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920715</creationdate><title>Amplification of P450c21 expression in cultured mammalian cells</title><author>Ricketts, Michael H. ; Chiao, Eric ; Hu, Meng-Chun ; Chung, Bon-chu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-db1588953b8b2847df7d8c9d624e77b4e41f99f72da0053d86d7a0aa31c52e793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Clone Cells</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Amplification</topic><topic>Genes, Regulator</topic><topic>Kinetics</topic><topic>Mammary Tumor Virus, Mouse - genetics</topic><topic>Oxidoreductases</topic><topic>Plasmids</topic><topic>Promoter Regions, Genetic</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Simian virus 40 - genetics</topic><topic>Steroid 21-Hydroxylase - genetics</topic><topic>Steroid 21-Hydroxylase - isolation & purification</topic><topic>Steroid 21-Hydroxylase - metabolism</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ricketts, Michael H.</creatorcontrib><creatorcontrib>Chiao, Eric</creatorcontrib><creatorcontrib>Hu, Meng-Chun</creatorcontrib><creatorcontrib>Chung, Bon-chu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ricketts, Michael H.</au><au>Chiao, Eric</au><au>Hu, Meng-Chun</au><au>Chung, Bon-chu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amplification of P450c21 expression in cultured mammalian cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1992-07-15</date><risdate>1992</risdate><volume>186</volume><issue>1</issue><spage>426</spage><epage>431</epage><pages>426-431</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>We describe in this paper an investigation of mammalian expression systems for P450c21 (21-hydroxylase). 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| ispartof | Biochemical and biophysical research communications, 1992-07, Vol.186 (1), p.426-431 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cell Line Clone Cells Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Gene Amplification Genes, Regulator Kinetics Mammary Tumor Virus, Mouse - genetics Oxidoreductases Plasmids Promoter Regions, Genetic Repetitive Sequences, Nucleic Acid Simian virus 40 - genetics Steroid 21-Hydroxylase - genetics Steroid 21-Hydroxylase - isolation & purification Steroid 21-Hydroxylase - metabolism Transfection |
| title | Amplification of P450c21 expression in cultured mammalian cells |
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