Chitosan enhances platelet adhesion and aggregation
In this study, chitosan (MW=50,000) was tested for its enhancing platelet activity in rabbit platelet suspensions and the possible mechanisms involved were further investigated. Our results showed that after initial (5 min) and long-term (30 min) contact of platelets with chitosan, the platelet adhe...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2003-03, Vol.302 (3), p.480-483 |
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| creator | Chou, Tz-Chong Fu, Earl Wu, Chang-Jer Yeh, Jeng-Hsien |
| description | In this study, chitosan (MW=50,000) was tested for its enhancing platelet activity in rabbit platelet suspensions and the possible mechanisms involved were further investigated. Our results showed that after initial (5
min) and long-term (30
min) contact of platelets with chitosan, the platelet adhesion to chitosan-coated microtiter plates was dose-dependently increased compared to that of solvent control. Similarly, chitosan also dose-dependently increased the platelet aggregation and the intracellular free Ca
2+ rise of Fura-2-AM loaded platelets. Additionally, in the presence of FITC-labeled anti-CD41/CD61, chitosan significantly enhanced the expression of platelet glycoprotein IIb/IIIa complex assayed by a flow cytometer. It is concluded that chitosan is an effective inducer for platelet adhesion and aggregation and the mechanisms of action of chitosan may be associated, at least partly, with the increasing [Ca
2+]
i mobilization and enhancing expression of GPIIb/IIIa complex on platelet membrane surfaces. |
| doi_str_mv | 10.1016/S0006-291X(03)00173-6 |
| format | Article |
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min) and long-term (30
min) contact of platelets with chitosan, the platelet adhesion to chitosan-coated microtiter plates was dose-dependently increased compared to that of solvent control. Similarly, chitosan also dose-dependently increased the platelet aggregation and the intracellular free Ca
2+ rise of Fura-2-AM loaded platelets. Additionally, in the presence of FITC-labeled anti-CD41/CD61, chitosan significantly enhanced the expression of platelet glycoprotein IIb/IIIa complex assayed by a flow cytometer. It is concluded that chitosan is an effective inducer for platelet adhesion and aggregation and the mechanisms of action of chitosan may be associated, at least partly, with the increasing [Ca
2+]
i mobilization and enhancing expression of GPIIb/IIIa complex on platelet membrane surfaces.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/S0006-291X(03)00173-6</identifier><identifier>PMID: 12615058</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Aggregation ; Animals ; Calcium - metabolism ; Cell Adhesion - drug effects ; Chitin - analogs & derivatives ; Chitin - metabolism ; Chitin - pharmacology ; Chitosan ; Dose-Response Relationship, Drug ; Flow Cytometry ; Fluorescent Dyes - pharmacology ; Glycoprotein IIb/IIIa ; Integrin beta3 - biosynthesis ; Intracellular calcium level ; Platelet adhesion ; Platelet Aggregation - drug effects ; Platelet Glycoprotein GPIIb-IIIa Complex - metabolism ; Platelet Membrane Glycoprotein IIb - biosynthesis ; Rabbits ; Temperature ; Time Factors</subject><ispartof>Biochemical and biophysical research communications, 2003-03, Vol.302 (3), p.480-483</ispartof><rights>2003 Elsevier Science (USA)</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-d3f5a4fd1b7f52132e148e0bd0ca49d16bf6f323d8cf5ac2bea8fca01b6b76323</citedby><cites>FETCH-LOGICAL-c413t-d3f5a4fd1b7f52132e148e0bd0ca49d16bf6f323d8cf5ac2bea8fca01b6b76323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-291X(03)00173-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12615058$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chou, Tz-Chong</creatorcontrib><creatorcontrib>Fu, Earl</creatorcontrib><creatorcontrib>Wu, Chang-Jer</creatorcontrib><creatorcontrib>Yeh, Jeng-Hsien</creatorcontrib><title>Chitosan enhances platelet adhesion and aggregation</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>In this study, chitosan (MW=50,000) was tested for its enhancing platelet activity in rabbit platelet suspensions and the possible mechanisms involved were further investigated. Our results showed that after initial (5
min) and long-term (30
min) contact of platelets with chitosan, the platelet adhesion to chitosan-coated microtiter plates was dose-dependently increased compared to that of solvent control. Similarly, chitosan also dose-dependently increased the platelet aggregation and the intracellular free Ca
2+ rise of Fura-2-AM loaded platelets. Additionally, in the presence of FITC-labeled anti-CD41/CD61, chitosan significantly enhanced the expression of platelet glycoprotein IIb/IIIa complex assayed by a flow cytometer. It is concluded that chitosan is an effective inducer for platelet adhesion and aggregation and the mechanisms of action of chitosan may be associated, at least partly, with the increasing [Ca
2+]
i mobilization and enhancing expression of GPIIb/IIIa complex on platelet membrane surfaces.</description><subject>Aggregation</subject><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Cell Adhesion - drug effects</subject><subject>Chitin - analogs & derivatives</subject><subject>Chitin - metabolism</subject><subject>Chitin - pharmacology</subject><subject>Chitosan</subject><subject>Dose-Response Relationship, Drug</subject><subject>Flow Cytometry</subject><subject>Fluorescent Dyes - pharmacology</subject><subject>Glycoprotein IIb/IIIa</subject><subject>Integrin beta3 - biosynthesis</subject><subject>Intracellular calcium level</subject><subject>Platelet adhesion</subject><subject>Platelet Aggregation - drug effects</subject><subject>Platelet Glycoprotein GPIIb-IIIa Complex - metabolism</subject><subject>Platelet Membrane Glycoprotein IIb - biosynthesis</subject><subject>Rabbits</subject><subject>Temperature</subject><subject>Time Factors</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMo7rr6E5SeRA_VmaZNtyeRxS9Y8KCCt5Amk91It12TruC_N_uBHj0NwzzvDPMwdopwhYDi-gUARJpV-H4B_BIAS56KPTZEqCDNEPJ9NvxFBuwohI8IYS6qQzbATGABxXjI-GTu-i6oNqF2rlpNIVk2qqeG-kSZOQXXtYlqTaJmM08z1cf-mB1Y1QQ62dURe7u_e508ptPnh6fJ7TTVOfI-NdwWKrcG69IWGfKMMB8T1Aa0yiuDorbC8oybsY6gzmpSY6sVYC3qUsTBiJ1v9y5997mi0MuFC5qaRrXUrYIsORQiE0UEiy2ofReCJyuX3i2U_5YIcm1LbmzJtQoJXG5sSRFzZ7sDq3pB5i-10xOBmy1A8c0vR14G7ShaMs6T7qXp3D8nfgDUsnoH</recordid><startdate>20030314</startdate><enddate>20030314</enddate><creator>Chou, Tz-Chong</creator><creator>Fu, Earl</creator><creator>Wu, Chang-Jer</creator><creator>Yeh, Jeng-Hsien</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030314</creationdate><title>Chitosan enhances platelet adhesion and aggregation</title><author>Chou, Tz-Chong ; Fu, Earl ; Wu, Chang-Jer ; Yeh, Jeng-Hsien</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-d3f5a4fd1b7f52132e148e0bd0ca49d16bf6f323d8cf5ac2bea8fca01b6b76323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Aggregation</topic><topic>Animals</topic><topic>Calcium - metabolism</topic><topic>Cell Adhesion - drug effects</topic><topic>Chitin - analogs & derivatives</topic><topic>Chitin - metabolism</topic><topic>Chitin - pharmacology</topic><topic>Chitosan</topic><topic>Dose-Response Relationship, Drug</topic><topic>Flow Cytometry</topic><topic>Fluorescent Dyes - pharmacology</topic><topic>Glycoprotein IIb/IIIa</topic><topic>Integrin beta3 - biosynthesis</topic><topic>Intracellular calcium level</topic><topic>Platelet adhesion</topic><topic>Platelet Aggregation - drug effects</topic><topic>Platelet Glycoprotein GPIIb-IIIa Complex - metabolism</topic><topic>Platelet Membrane Glycoprotein IIb - biosynthesis</topic><topic>Rabbits</topic><topic>Temperature</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chou, Tz-Chong</creatorcontrib><creatorcontrib>Fu, Earl</creatorcontrib><creatorcontrib>Wu, Chang-Jer</creatorcontrib><creatorcontrib>Yeh, Jeng-Hsien</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chou, Tz-Chong</au><au>Fu, Earl</au><au>Wu, Chang-Jer</au><au>Yeh, Jeng-Hsien</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chitosan enhances platelet adhesion and aggregation</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2003-03-14</date><risdate>2003</risdate><volume>302</volume><issue>3</issue><spage>480</spage><epage>483</epage><pages>480-483</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>In this study, chitosan (MW=50,000) was tested for its enhancing platelet activity in rabbit platelet suspensions and the possible mechanisms involved were further investigated. Our results showed that after initial (5
min) and long-term (30
min) contact of platelets with chitosan, the platelet adhesion to chitosan-coated microtiter plates was dose-dependently increased compared to that of solvent control. Similarly, chitosan also dose-dependently increased the platelet aggregation and the intracellular free Ca
2+ rise of Fura-2-AM loaded platelets. Additionally, in the presence of FITC-labeled anti-CD41/CD61, chitosan significantly enhanced the expression of platelet glycoprotein IIb/IIIa complex assayed by a flow cytometer. It is concluded that chitosan is an effective inducer for platelet adhesion and aggregation and the mechanisms of action of chitosan may be associated, at least partly, with the increasing [Ca
2+]
i mobilization and enhancing expression of GPIIb/IIIa complex on platelet membrane surfaces.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>12615058</pmid><doi>10.1016/S0006-291X(03)00173-6</doi><tpages>4</tpages></addata></record> |
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| subjects | Aggregation Animals Calcium - metabolism Cell Adhesion - drug effects Chitin - analogs & derivatives Chitin - metabolism Chitin - pharmacology Chitosan Dose-Response Relationship, Drug Flow Cytometry Fluorescent Dyes - pharmacology Glycoprotein IIb/IIIa Integrin beta3 - biosynthesis Intracellular calcium level Platelet adhesion Platelet Aggregation - drug effects Platelet Glycoprotein GPIIb-IIIa Complex - metabolism Platelet Membrane Glycoprotein IIb - biosynthesis Rabbits Temperature Time Factors |
| title | Chitosan enhances platelet adhesion and aggregation |
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