Influence of extracellular pH and perfusion rate on Na+/H+ exchange in cultured opossum kidney cells
Studies were undertaken in cultured opossum kidney (OK) cells to determine whether the rate of H+ secretion by apical membrane Na+/H+ exchange is modulated by changes in extracellular pH or perfusion rate. H+ secretion was assessed in single cells by measuring the rate of Na(+)-dependent intracellul...
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Veröffentlicht in: | Pflügers Archiv 1992-02, Vol.420 (2), p.153-158 |
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description | Studies were undertaken in cultured opossum kidney (OK) cells to determine whether the rate of H+ secretion by apical membrane Na+/H+ exchange is modulated by changes in extracellular pH or perfusion rate. H+ secretion was assessed in single cells by measuring the rate of Na(+)-dependent intracellular pH recovery after NH4Cl loading, using the pH-sensitive fluorescent dye, 2'7'-bis(carboxyethyl)-5,6-carboxyfluorescein, in monolayers mounted to allow independent perfusion of the apical and basolateral surfaces. At constant intracellular pH, Na(+)-dependent H+ secretion was found to be inversely related to extracellular H+ activity, and directly related to the perfusate flow rate. Inhibition of H+ secretion by perfusate acidity occurred immediately and was greater when perfusate Na+ was reduced, consistent with H+ competition with Na+ for binding to the transporter. By contrast, the effect of the perfusion rate was a delayed response, requiring 20 min of exposure, and was independent of perfusate Na+ concentration. The results indicate that both extracellular pH and the perfusion rate modulate H+ secretion by OK cells, and that the two effects are independent. |
doi_str_mv | 10.1007/BF00374984 |
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Inhibition of H+ secretion by perfusate acidity occurred immediately and was greater when perfusate Na+ was reduced, consistent with H+ competition with Na+ for binding to the transporter. By contrast, the effect of the perfusion rate was a delayed response, requiring 20 min of exposure, and was independent of perfusate Na+ concentration. The results indicate that both extracellular pH and the perfusion rate modulate H+ secretion by OK cells, and that the two effects are independent.</description><identifier>ISSN: 0031-6768</identifier><identifier>EISSN: 1432-2013</identifier><identifier>DOI: 10.1007/BF00374984</identifier><identifier>PMID: 1320250</identifier><identifier>CODEN: PFLABK</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Animals ; Biological and medical sciences ; Buffers ; Carrier Proteins - metabolism ; Cell Line ; Extracellular Space - physiology ; Fundamental and applied biological sciences. 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J</creatorcontrib><creatorcontrib>HELMLE-KOLB, C</creatorcontrib><creatorcontrib>MURER, H</creatorcontrib><title>Influence of extracellular pH and perfusion rate on Na+/H+ exchange in cultured opossum kidney cells</title><title>Pflügers Archiv</title><addtitle>Pflugers Arch</addtitle><description>Studies were undertaken in cultured opossum kidney (OK) cells to determine whether the rate of H+ secretion by apical membrane Na+/H+ exchange is modulated by changes in extracellular pH or perfusion rate. H+ secretion was assessed in single cells by measuring the rate of Na(+)-dependent intracellular pH recovery after NH4Cl loading, using the pH-sensitive fluorescent dye, 2'7'-bis(carboxyethyl)-5,6-carboxyfluorescein, in monolayers mounted to allow independent perfusion of the apical and basolateral surfaces. At constant intracellular pH, Na(+)-dependent H+ secretion was found to be inversely related to extracellular H+ activity, and directly related to the perfusate flow rate. Inhibition of H+ secretion by perfusate acidity occurred immediately and was greater when perfusate Na+ was reduced, consistent with H+ competition with Na+ for binding to the transporter. By contrast, the effect of the perfusion rate was a delayed response, requiring 20 min of exposure, and was independent of perfusate Na+ concentration. The results indicate that both extracellular pH and the perfusion rate modulate H+ secretion by OK cells, and that the two effects are independent.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Buffers</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell Line</subject><subject>Extracellular Space - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen - metabolism</subject><subject>Hydrogen-Ion Concentration</subject><subject>Kidney - cytology</subject><subject>Kidney - metabolism</subject><subject>Kinetics</subject><subject>Opossums</subject><subject>Perfusion</subject><subject>Sodium - metabolism</subject><subject>Sodium-Hydrogen Exchangers</subject><subject>Vertebrates: urinary system</subject><issn>0031-6768</issn><issn>1432-2013</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMFLwzAUxoMoOqcX70IO4kGpviTt0h11ODcYetFzyZIXrbZpTRrQ_96MDT09eN-PHx8fIWcMbhiAvL2fAwiZT8t8j4xYLnjGgYl9Mkpvlk3kpDwixyF8AADPS35IDpngwAsYEbN0tonoNNLOUvwevNLYNLFRnvYLqpyhPXobQ9056tWQMEef1PXt4jrR-l25N6S1ozo2Q_RoaNd3IcSWftbG4Q_dyMIJObCqCXi6u2PyOn94mS2y1fPjcna3yrRgbMh0oXNTirXmODXMSKasyYuC5cA5FCgFS5UtM0qJNU6kVVaWnMHUojBTLEGMyeXW2_vuK2IYqrYOmwbKYRdDJQUkHS8SeLUFtU9tPdqq93Wr_E_FoNpMWv1PmuDznTWuWzT_6HbDlF_schW0aqxXTtfhDys4y6UQ4hfObXz7</recordid><startdate>19920201</startdate><enddate>19920201</enddate><creator>GENNARI, F. 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J ; HELMLE-KOLB, C ; MURER, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c311t-c5c4d83bc2e9d1d71afd4551402205e731250f1daa3be67faf782109fe3d9e803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Buffers</topic><topic>Carrier Proteins - metabolism</topic><topic>Cell Line</topic><topic>Extracellular Space - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen - metabolism</topic><topic>Hydrogen-Ion Concentration</topic><topic>Kidney - cytology</topic><topic>Kidney - metabolism</topic><topic>Kinetics</topic><topic>Opossums</topic><topic>Perfusion</topic><topic>Sodium - metabolism</topic><topic>Sodium-Hydrogen Exchangers</topic><topic>Vertebrates: urinary system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GENNARI, F. J</creatorcontrib><creatorcontrib>HELMLE-KOLB, C</creatorcontrib><creatorcontrib>MURER, H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pflügers Archiv</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GENNARI, F. J</au><au>HELMLE-KOLB, C</au><au>MURER, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of extracellular pH and perfusion rate on Na+/H+ exchange in cultured opossum kidney cells</atitle><jtitle>Pflügers Archiv</jtitle><addtitle>Pflugers Arch</addtitle><date>1992-02-01</date><risdate>1992</risdate><volume>420</volume><issue>2</issue><spage>153</spage><epage>158</epage><pages>153-158</pages><issn>0031-6768</issn><eissn>1432-2013</eissn><coden>PFLABK</coden><abstract>Studies were undertaken in cultured opossum kidney (OK) cells to determine whether the rate of H+ secretion by apical membrane Na+/H+ exchange is modulated by changes in extracellular pH or perfusion rate. H+ secretion was assessed in single cells by measuring the rate of Na(+)-dependent intracellular pH recovery after NH4Cl loading, using the pH-sensitive fluorescent dye, 2'7'-bis(carboxyethyl)-5,6-carboxyfluorescein, in monolayers mounted to allow independent perfusion of the apical and basolateral surfaces. At constant intracellular pH, Na(+)-dependent H+ secretion was found to be inversely related to extracellular H+ activity, and directly related to the perfusate flow rate. Inhibition of H+ secretion by perfusate acidity occurred immediately and was greater when perfusate Na+ was reduced, consistent with H+ competition with Na+ for binding to the transporter. By contrast, the effect of the perfusion rate was a delayed response, requiring 20 min of exposure, and was independent of perfusate Na+ concentration. The results indicate that both extracellular pH and the perfusion rate modulate H+ secretion by OK cells, and that the two effects are independent.</abstract><cop>Heidelberg</cop><pub>Springer</pub><pmid>1320250</pmid><doi>10.1007/BF00374984</doi><tpages>6</tpages></addata></record> |
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subjects | Animals Biological and medical sciences Buffers Carrier Proteins - metabolism Cell Line Extracellular Space - physiology Fundamental and applied biological sciences. Psychology Hydrogen - metabolism Hydrogen-Ion Concentration Kidney - cytology Kidney - metabolism Kinetics Opossums Perfusion Sodium - metabolism Sodium-Hydrogen Exchangers Vertebrates: urinary system |
title | Influence of extracellular pH and perfusion rate on Na+/H+ exchange in cultured opossum kidney cells |
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