Amplicon Melting Analysis with Labeled Primers: A Closed-Tube Method for Differentiating Homozygotes and Heterozygotes

Common methods for identification of DNA sequence variants use gel electrophoresis or column separation after PCR. We developed a method for sequence variant analysis requiring only PCR and amplicon melting analysis. One of the PCR primers was fluorescently labeled. After PCR, the melting transition...

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Veröffentlicht in:	Clinical chemistry (Baltimore, Md.) Md.), 2003-03, Vol.49 (3), p.396-406
Hauptverfasser:	Gundry, Cameron N, Vandersteen, Joshua G, Reed, Gudrun H, Pryor, Robert J, Chen, Jian, Wittwer, Carl T
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences Blood Proteins - genetics Cystic fibrosis Cystic Fibrosis - genetics Cystic Fibrosis Transmembrane Conductance Regulator Deoxyribonucleic acid DNA DNA - genetics Fluorescent Dyes General aspects. Genetic counseling Genotype Globins - genetics Heteroduplex Analysis Heterozygote High-Temperature Requirement A Serine Peptidase 2 Homozygote Humans Low temperature Medical genetics Medical sciences Melting Methods Mitochondrial Proteins Mutation Oligonucleotide Probes Organic Chemicals Polymerase Chain Reaction - methods Polymorphism Polymorphism, Genetic Serine Endopeptidases - genetics Temperature
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