Establishment and characterization of a mouse Schwann cell line which produces myelin in vivo
A Schwann cell line (MSC 80) was established from purified mouse Schwann cell cultures using large doses of serum. MSC 80 cell line is an aneuploid cell line which has a doubling time of 17 hr and has been maintained through more than 110 passages. Most of MSC 80 cells are of bipolar or stellate (3–...
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| Veröffentlicht in: | Journal of neuroscience research 1992-05, Vol.32 (1), p.15-26 |
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| creator | Boutry, J.-M. Hauw, J.-J. Gansmüller, A. Di-Bert, N. Pouchelet, M. Baron-Van Evercooren, A. |
| description | A Schwann cell line (MSC 80) was established from purified mouse Schwann cell cultures using large doses of serum. MSC 80 cell line is an aneuploid cell line which has a doubling time of 17 hr and has been maintained through more than 110 passages. Most of MSC 80 cells are of bipolar or stellate (3–5 processes) shape. A few others are irregular in shape, gigantic, and multinucleated. All MSC 80 cells express antigens of myelin‐forming Schwann cells such as S‐100, 224/58, laminin, and other glycoproteins of the extracellular matrix. However, they also express the nonmyelin‐forming Schwann cell antigen GFAP. By time‐lapse cinematography, MSC 80 cells exhibit the Schwann cell characteristic rhythmical undulations. When induced to form aggregates in agar, they form intercellular junctions and basement membrane‐like structures. In addition, after transplantation in or at a distance from a lysolecithin induced lesion, MSC 80 cells from myelin around the host demyelinated axons. MSC 80 cells thus express, when isolated in vitro, some of the normal myelin‐forming Schwann cell phenotype. In addition, they present the major advantage of forming myelin when associated with axons in vivo. © 1992 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/jnr.490320103 |
| format | Article |
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MSC 80 cell line is an aneuploid cell line which has a doubling time of 17 hr and has been maintained through more than 110 passages. Most of MSC 80 cells are of bipolar or stellate (3–5 processes) shape. A few others are irregular in shape, gigantic, and multinucleated. All MSC 80 cells express antigens of myelin‐forming Schwann cells such as S‐100, 224/58, laminin, and other glycoproteins of the extracellular matrix. However, they also express the nonmyelin‐forming Schwann cell antigen GFAP. By time‐lapse cinematography, MSC 80 cells exhibit the Schwann cell characteristic rhythmical undulations. When induced to form aggregates in agar, they form intercellular junctions and basement membrane‐like structures. In addition, after transplantation in or at a distance from a lysolecithin induced lesion, MSC 80 cells from myelin around the host demyelinated axons. MSC 80 cells thus express, when isolated in vitro, some of the normal myelin‐forming Schwann cell phenotype. In addition, they present the major advantage of forming myelin when associated with axons in vivo. © 1992 Wiley‐Liss, Inc.</description><identifier>ISSN: 0360-4012</identifier><identifier>EISSN: 1097-4547</identifier><identifier>DOI: 10.1002/jnr.490320103</identifier><identifier>PMID: 1629940</identifier><identifier>CODEN: JNREDK</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animal cells ; Animals ; Benzimidazoles ; Biological and medical sciences ; Biomarkers ; Biotechnology ; Cell Line ; Cell Movement ; demyelination ; Establishment of new cell lines, improvement of cultural methods, mass cultures ; Eukaryotic cell cultures ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Glial Fibrillary Acidic Protein - metabolism ; Methods. Procedures. Technologies ; Mice ; MSC 80 ; Myelin Sheath - metabolism ; myelination ; Phenotype ; remyelination ; S100 Proteins - metabolism ; Schwann Cells - metabolism ; Schwann Cells - physiology ; Schwann Cells - ultrastructure ; Sciatic Nerve - cytology ; transplantation</subject><ispartof>Journal of neuroscience research, 1992-05, Vol.32 (1), p.15-26</ispartof><rights>Copyright © 1992 Wiley‐Liss, Inc.</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3493-ecbd85eb37af3350cc2faa6f9f6c240fff4d97e407cb1c9de0aa6eb9412e80fe3</citedby><cites>FETCH-LOGICAL-c3493-ecbd85eb37af3350cc2faa6f9f6c240fff4d97e407cb1c9de0aa6eb9412e80fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjnr.490320103$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjnr.490320103$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5304442$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1629940$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boutry, J.-M.</creatorcontrib><creatorcontrib>Hauw, J.-J.</creatorcontrib><creatorcontrib>Gansmüller, A.</creatorcontrib><creatorcontrib>Di-Bert, N.</creatorcontrib><creatorcontrib>Pouchelet, M.</creatorcontrib><creatorcontrib>Baron-Van Evercooren, A.</creatorcontrib><title>Establishment and characterization of a mouse Schwann cell line which produces myelin in vivo</title><title>Journal of neuroscience research</title><addtitle>J. Neurosci. Res</addtitle><description>A Schwann cell line (MSC 80) was established from purified mouse Schwann cell cultures using large doses of serum. MSC 80 cell line is an aneuploid cell line which has a doubling time of 17 hr and has been maintained through more than 110 passages. Most of MSC 80 cells are of bipolar or stellate (3–5 processes) shape. A few others are irregular in shape, gigantic, and multinucleated. All MSC 80 cells express antigens of myelin‐forming Schwann cells such as S‐100, 224/58, laminin, and other glycoproteins of the extracellular matrix. However, they also express the nonmyelin‐forming Schwann cell antigen GFAP. By time‐lapse cinematography, MSC 80 cells exhibit the Schwann cell characteristic rhythmical undulations. When induced to form aggregates in agar, they form intercellular junctions and basement membrane‐like structures. In addition, after transplantation in or at a distance from a lysolecithin induced lesion, MSC 80 cells from myelin around the host demyelinated axons. MSC 80 cells thus express, when isolated in vitro, some of the normal myelin‐forming Schwann cell phenotype. In addition, they present the major advantage of forming myelin when associated with axons in vivo. © 1992 Wiley‐Liss, Inc.</description><subject>Animal cells</subject><subject>Animals</subject><subject>Benzimidazoles</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Biotechnology</subject><subject>Cell Line</subject><subject>Cell Movement</subject><subject>demyelination</subject><subject>Establishment of new cell lines, improvement of cultural methods, mass cultures</subject><subject>Eukaryotic cell cultures</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glial Fibrillary Acidic Protein - metabolism</subject><subject>Methods. Procedures. Technologies</subject><subject>Mice</subject><subject>MSC 80</subject><subject>Myelin Sheath - metabolism</subject><subject>myelination</subject><subject>Phenotype</subject><subject>remyelination</subject><subject>S100 Proteins - metabolism</subject><subject>Schwann Cells - metabolism</subject><subject>Schwann Cells - physiology</subject><subject>Schwann Cells - ultrastructure</subject><subject>Sciatic Nerve - cytology</subject><subject>transplantation</subject><issn>0360-4012</issn><issn>1097-4547</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFLHDEUh4O02NV69CjkUHob-zLJTDZHWay2iKXW0lMJmcwLE53J2GTW7favb2SXrScLgUB-X977-BFyzOCUAZQf7kI8FQp4CQz4HpkxULIQlZCvyAx4DYUAVr4hByndAYBSFd8n-6wulRIwIz_P02Sa3qduwDBRE1pqOxONnTD6P2byY6Cjo4YO4zIh_Wa7lQmBWux72vuAdNV529GHOLZLi4kOa8zPNJ9H_zi-Ja-d6RMebe9D8v3j-e3isrj6cvFpcXZVWC4UL9A27bzChkvjOK_A2tIZUzvlalsKcM6JVkkUIG3DrGoRcoqNEqzEOTjkh-T9Zm72-LXENOnBpydHEzB7a8mh4lDDf0FWMyWFmmew2IA2jilFdPoh-sHEtWagn3rXuXe96z3zJ9vBy2bA9h-9KTrn77a5Sdb0Lppgfdph2U4IUWZMbrCV73H98k79-frmucBW2KcJf-9-mniva8llpX9cX-ivi1s1F2Khb_hf-jirxQ</recordid><startdate>199205</startdate><enddate>199205</enddate><creator>Boutry, J.-M.</creator><creator>Hauw, J.-J.</creator><creator>Gansmüller, A.</creator><creator>Di-Bert, N.</creator><creator>Pouchelet, M.</creator><creator>Baron-Van Evercooren, A.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>199205</creationdate><title>Establishment and characterization of a mouse Schwann cell line which produces myelin in vivo</title><author>Boutry, J.-M. ; Hauw, J.-J. ; Gansmüller, A. ; Di-Bert, N. ; Pouchelet, M. ; Baron-Van Evercooren, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3493-ecbd85eb37af3350cc2faa6f9f6c240fff4d97e407cb1c9de0aa6eb9412e80fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animal cells</topic><topic>Animals</topic><topic>Benzimidazoles</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Biotechnology</topic><topic>Cell Line</topic><topic>Cell Movement</topic><topic>demyelination</topic><topic>Establishment of new cell lines, improvement of cultural methods, mass cultures</topic><topic>Eukaryotic cell cultures</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glial Fibrillary Acidic Protein - metabolism</topic><topic>Methods. Procedures. Technologies</topic><topic>Mice</topic><topic>MSC 80</topic><topic>Myelin Sheath - metabolism</topic><topic>myelination</topic><topic>Phenotype</topic><topic>remyelination</topic><topic>S100 Proteins - metabolism</topic><topic>Schwann Cells - metabolism</topic><topic>Schwann Cells - physiology</topic><topic>Schwann Cells - ultrastructure</topic><topic>Sciatic Nerve - cytology</topic><topic>transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boutry, J.-M.</creatorcontrib><creatorcontrib>Hauw, J.-J.</creatorcontrib><creatorcontrib>Gansmüller, A.</creatorcontrib><creatorcontrib>Di-Bert, N.</creatorcontrib><creatorcontrib>Pouchelet, M.</creatorcontrib><creatorcontrib>Baron-Van Evercooren, A.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroscience research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boutry, J.-M.</au><au>Hauw, J.-J.</au><au>Gansmüller, A.</au><au>Di-Bert, N.</au><au>Pouchelet, M.</au><au>Baron-Van Evercooren, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment and characterization of a mouse Schwann cell line which produces myelin in vivo</atitle><jtitle>Journal of neuroscience research</jtitle><addtitle>J. Neurosci. Res</addtitle><date>1992-05</date><risdate>1992</risdate><volume>32</volume><issue>1</issue><spage>15</spage><epage>26</epage><pages>15-26</pages><issn>0360-4012</issn><eissn>1097-4547</eissn><coden>JNREDK</coden><abstract>A Schwann cell line (MSC 80) was established from purified mouse Schwann cell cultures using large doses of serum. MSC 80 cell line is an aneuploid cell line which has a doubling time of 17 hr and has been maintained through more than 110 passages. Most of MSC 80 cells are of bipolar or stellate (3–5 processes) shape. A few others are irregular in shape, gigantic, and multinucleated. All MSC 80 cells express antigens of myelin‐forming Schwann cells such as S‐100, 224/58, laminin, and other glycoproteins of the extracellular matrix. However, they also express the nonmyelin‐forming Schwann cell antigen GFAP. By time‐lapse cinematography, MSC 80 cells exhibit the Schwann cell characteristic rhythmical undulations. When induced to form aggregates in agar, they form intercellular junctions and basement membrane‐like structures. In addition, after transplantation in or at a distance from a lysolecithin induced lesion, MSC 80 cells from myelin around the host demyelinated axons. MSC 80 cells thus express, when isolated in vitro, some of the normal myelin‐forming Schwann cell phenotype. In addition, they present the major advantage of forming myelin when associated with axons in vivo. © 1992 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>1629940</pmid><doi>10.1002/jnr.490320103</doi><tpages>12</tpages></addata></record> |
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| ispartof | Journal of neuroscience research, 1992-05, Vol.32 (1), p.15-26 |
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| subjects | Animal cells Animals Benzimidazoles Biological and medical sciences Biomarkers Biotechnology Cell Line Cell Movement demyelination Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Glial Fibrillary Acidic Protein - metabolism Methods. Procedures. Technologies Mice MSC 80 Myelin Sheath - metabolism myelination Phenotype remyelination S100 Proteins - metabolism Schwann Cells - metabolism Schwann Cells - physiology Schwann Cells - ultrastructure Sciatic Nerve - cytology transplantation |
| title | Establishment and characterization of a mouse Schwann cell line which produces myelin in vivo |
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