Development of a microplate assay for the detection of single plaque-forming units of bacteriophage PhiX174 in crude lysates

Mice containing the PhiX174 am3 transgene can be used for measuring in vivo mutation; however, the single burst analysis method used for distinguishing in vivo mutations from mutations generated during sample processing is labor-intensive. A liquid microplate assay was developed that detects a singl...

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Veröffentlicht in:	Environmental and molecular mutagenesis 2003, Vol.41 (2), p.121-125
Hauptverfasser:	Slattery, Scott D, Valentine, Carrie R
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteriophage phi X 174 - growth & development Cell Division Colony Count, Microbial - methods Mice Mice, Transgenic Sensitivity and Specificity Tetrazolium Salts - metabolism Transgenes Viral Plaque Assay - methods
Online-Zugang:	Volltext
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