An interaction between an Arabidopsis poly(A) polymerase and a homologue of the 100 kDa subunit of CPSF

The Arabidopsis genome possesses a number of sequences that are predicted to encode proteins that are similar to mammalian and yeast polyadenylation factor subunits. One of these resides on chromosome V and has the potential to encode a polypeptide related to the 100 kDa subunit of the mammalian cle...

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Veröffentlicht in:Plant molecular biology 2003-02, Vol.51 (3), p.373-384
Hauptverfasser: Elliott, Barbara J, Dattaroy, Tomal, Meeks-Midkiff, Lisa R, Forbes, Kevin P, Hunt, Arthur G
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container_issue 3
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container_title Plant molecular biology
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creator Elliott, Barbara J
Dattaroy, Tomal
Meeks-Midkiff, Lisa R
Forbes, Kevin P
Hunt, Arthur G
description The Arabidopsis genome possesses a number of sequences that are predicted to encode proteins that are similar to mammalian and yeast polyadenylation factor subunits. One of these resides on chromosome V and has the potential to encode a polypeptide related to the 100 kDa subunit of the mammalian cleavage and polyadenylation specificity factor (CPSF). This gene encodes a ca. 2400 nucleotide mRNA that in turn can be translated to yield a polypeptide that is 39% identical to the mammalian CPSF100 protein. Antibodies raised against the Arabidopsis protein recognized distinctive polypeptides in nuclear extracts prepared from pea and wheat germ, consistent with the hypothesis that the Arabidopsis protein is resident in a nuclear polyadenylation complex. Interestingly, the Arabidopsis CPSF100 was found to interact with a portion of a nuclear poly(A) polymerase. This interaction was attributable to a 60 amino acid domain in the CPSF100 polypeptide and the N-terminal 220 amino acids of the poly(A) polymerase. An analogous interaction has yet to be described in other eukaryotes. The interaction with PAP thus indicates that the plant CPSF100 polypeptide is likely part of the 3'-end processing machinery, but suggests that this complex may function differently in plants than it does in mammals and yeast.
doi_str_mv 10.1023/A:1022035219500
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subjects Amino Acid Sequence
Amino acids
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Cleavage And Polyadenylation Specificity Factor - genetics
Cleavage And Polyadenylation Specificity Factor - metabolism
Cloning, Molecular
DNA, Complementary - chemistry
DNA, Complementary - genetics
Mammals
Molecular Sequence Data
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Phylogeny
Polynucleotide Adenylyltransferase - genetics
Polynucleotide Adenylyltransferase - metabolism
Polypeptides
Protein Binding
Saccharomyces cerevisiae - genetics
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Two-Hybrid System Techniques
Yeasts
title An interaction between an Arabidopsis poly(A) polymerase and a homologue of the 100 kDa subunit of CPSF
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