Cobalt-Vitamin B-12 Deficiency Decreases Methionine Synthase Activity and Phospholipid Methylation in Sheep
Two groups of lambs were fed either a Co-deficient or a Co-sufficient whole barley-based diet for 28 wk to induce a severe Co-vitamin B-12 deficiency. Holo and apo methionine synthase activities were significantly lower in the liver, kidney and spinal cord of Co-deficient animals compared with contr...
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| Veröffentlicht in: | The Journal of nutrition 1992-07, Vol.122 (7), p.1384-1390 |
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| container_title | The Journal of nutrition |
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| creator | Kennedy, D. Glenn Blanchflower, W. John Scott, John M. Weir, Donald G. Molloy, Anne M. Kennedy, Seamus Young, Paul B. |
| description | Two groups of lambs were fed either a Co-deficient or a Co-sufficient whole barley-based diet for 28 wk to induce a severe Co-vitamin B-12 deficiency. Holo and apo methionine synthase activities were significantly lower in the liver, kidney and spinal cord of Co-deficient animals compared with controls. Neither form of this enzyme in the brain was affected by Co deficiency. The ratio of the tissue concentrations of S-adenosyl methionine to S-adenosyl homocysteine was significantly lower only in the liver of Co-deficient animals, suggesting that the activity of hepatic SAM-dependent methyltransferase enzymes would be impaired. Measurements of tissue concentrations of phosphatidyl choline and phosphatidyl ethanolamine revealed lower concentrations of phosphatidyl choline and a lower phosphatidyl choline:phosphatidyl ethanolamine ratio in both liver and brain of the Co-deficient animals. The latter finding occurred in the absence of changes in either methionine synthase activity or the methylation ratio and may result from impaired availability of hepatic phosphatidyl choline for transport into the brain. |
| doi_str_mv | 10.1093/jn/122.7.1384 |
| format | Article |
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Glenn ; Blanchflower, W. John ; Scott, John M. ; Weir, Donald G. ; Molloy, Anne M. ; Kennedy, Seamus ; Young, Paul B.</creator><creatorcontrib>Kennedy, D. Glenn ; Blanchflower, W. John ; Scott, John M. ; Weir, Donald G. ; Molloy, Anne M. ; Kennedy, Seamus ; Young, Paul B.</creatorcontrib><description>Two groups of lambs were fed either a Co-deficient or a Co-sufficient whole barley-based diet for 28 wk to induce a severe Co-vitamin B-12 deficiency. Holo and apo methionine synthase activities were significantly lower in the liver, kidney and spinal cord of Co-deficient animals compared with controls. Neither form of this enzyme in the brain was affected by Co deficiency. The ratio of the tissue concentrations of S-adenosyl methionine to S-adenosyl homocysteine was significantly lower only in the liver of Co-deficient animals, suggesting that the activity of hepatic SAM-dependent methyltransferase enzymes would be impaired. Measurements of tissue concentrations of phosphatidyl choline and phosphatidyl ethanolamine revealed lower concentrations of phosphatidyl choline and a lower phosphatidyl choline:phosphatidyl ethanolamine ratio in both liver and brain of the Co-deficient animals. The latter finding occurred in the absence of changes in either methionine synthase activity or the methylation ratio and may result from impaired availability of hepatic phosphatidyl choline for transport into the brain.</description><identifier>ISSN: 0022-3166</identifier><identifier>EISSN: 1541-6100</identifier><identifier>DOI: 10.1093/jn/122.7.1384</identifier><identifier>PMID: 1619466</identifier><identifier>CODEN: JONUAI</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase - metabolism ; ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; Animals ; Biological and medical sciences ; Brain - enzymology ; Brain - metabolism ; CARENCE EN VITAMINE ; CARENCE MINERALE ; COBALT ; Cobalt - deficiency ; COBALTO ; DEFICIENCIA DE MINERALES ; DEFICIENCIA DE VITAMINAS ; DIETA ; FOSFOLIPIDOS ; hypomethylation ; Kidney - enzymology ; Kidney - metabolism ; LIGASAS ; LIGASE ; Liver - enzymology ; Liver - metabolism ; Medical sciences ; Metabolic diseases ; METHIONINE ; METHYLATION ; METILACION ; METIONINA ; Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...) ; OVIN ; OVINOS ; PHOSPHATIDE ; phosphatidyl choline ; Phosphatidylcholines - metabolism ; Phosphatidylethanolamines - metabolism ; REGIME ALIMENTAIRE ; Sheep ; Vitamin B 12 Deficiency - enzymology ; Vitamin B 12 Deficiency - metabolism ; vitamin B-12 ; VITAMINA B12 ; VITAMINE B12</subject><ispartof>The Journal of nutrition, 1992-07, Vol.122 (7), p.1384-1390</ispartof><rights>1992 American Institute of Nutrition.</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c489t-fca4515c3256faee21e2941d1e0523f5b5d5c7664aba6a447b3f66a23a918ba23</citedby><cites>FETCH-LOGICAL-c489t-fca4515c3256faee21e2941d1e0523f5b5d5c7664aba6a447b3f66a23a918ba23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5546783$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1619466$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kennedy, D. Glenn</creatorcontrib><creatorcontrib>Blanchflower, W. John</creatorcontrib><creatorcontrib>Scott, John M.</creatorcontrib><creatorcontrib>Weir, Donald G.</creatorcontrib><creatorcontrib>Molloy, Anne M.</creatorcontrib><creatorcontrib>Kennedy, Seamus</creatorcontrib><creatorcontrib>Young, Paul B.</creatorcontrib><title>Cobalt-Vitamin B-12 Deficiency Decreases Methionine Synthase Activity and Phospholipid Methylation in Sheep</title><title>The Journal of nutrition</title><addtitle>J Nutr</addtitle><description>Two groups of lambs were fed either a Co-deficient or a Co-sufficient whole barley-based diet for 28 wk to induce a severe Co-vitamin B-12 deficiency. Holo and apo methionine synthase activities were significantly lower in the liver, kidney and spinal cord of Co-deficient animals compared with controls. Neither form of this enzyme in the brain was affected by Co deficiency. The ratio of the tissue concentrations of S-adenosyl methionine to S-adenosyl homocysteine was significantly lower only in the liver of Co-deficient animals, suggesting that the activity of hepatic SAM-dependent methyltransferase enzymes would be impaired. Measurements of tissue concentrations of phosphatidyl choline and phosphatidyl ethanolamine revealed lower concentrations of phosphatidyl choline and a lower phosphatidyl choline:phosphatidyl ethanolamine ratio in both liver and brain of the Co-deficient animals. The latter finding occurred in the absence of changes in either methionine synthase activity or the methylation ratio and may result from impaired availability of hepatic phosphatidyl choline for transport into the brain.</description><subject>5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase - metabolism</subject><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain - enzymology</subject><subject>Brain - metabolism</subject><subject>CARENCE EN VITAMINE</subject><subject>CARENCE MINERALE</subject><subject>COBALT</subject><subject>Cobalt - deficiency</subject><subject>COBALTO</subject><subject>DEFICIENCIA DE MINERALES</subject><subject>DEFICIENCIA DE VITAMINAS</subject><subject>DIETA</subject><subject>FOSFOLIPIDOS</subject><subject>hypomethylation</subject><subject>Kidney - enzymology</subject><subject>Kidney - metabolism</subject><subject>LIGASAS</subject><subject>LIGASE</subject><subject>Liver - enzymology</subject><subject>Liver - metabolism</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>METHIONINE</subject><subject>METHYLATION</subject><subject>METILACION</subject><subject>METIONINA</subject><subject>Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...)</subject><subject>OVIN</subject><subject>OVINOS</subject><subject>PHOSPHATIDE</subject><subject>phosphatidyl choline</subject><subject>Phosphatidylcholines - metabolism</subject><subject>Phosphatidylethanolamines - metabolism</subject><subject>REGIME ALIMENTAIRE</subject><subject>Sheep</subject><subject>Vitamin B 12 Deficiency - enzymology</subject><subject>Vitamin B 12 Deficiency - metabolism</subject><subject>vitamin B-12</subject><subject>VITAMINA B12</subject><subject>VITAMINE B12</subject><issn>0022-3166</issn><issn>1541-6100</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtv1DAURi0EKtPCkg0SUhaou0z9zmRZhqdUBNJQttaNc0M8ZJzU9lTKv8dtRrBidS1_x5-scwl5xeia0Vpc7f0V43xdrZnYyCdkxZRkpWaUPiUrSjkvBdP6OTmPcU8pZbLenJEzplkttV6R39uxgSGVP12Cg_PFu5Lx4j12zjr0ds5HGxAixuIrpt6N3nksdrNPfb4srm1y9y7NBfi2-N6PcerHwU2ufaTnAVJ-UeTaXY84vSDPOhgivjzNC3L78cOP7efy5tunL9vrm9LKTZ3KzoJUTFnBle4AkTPktWQtQ6q46FSjWmUrrSU0oEHKqhGd1sAF1GzT5HlBLpfeKYx3R4zJHFy0OAzgcTxGUwmaLVCawXIBbRhjDNiZKbgDhNkwah7kmr03Wa6pzIPczL85FR-bA7b_6MVmzt-ecogWhi6Aty7-xZSSutqIjL1esA5GA79CRm53taB5c1UOqyXEbOjeYTDxcRXYuoA2mXZ0__ndH8EEnMY</recordid><startdate>19920701</startdate><enddate>19920701</enddate><creator>Kennedy, D. Glenn</creator><creator>Blanchflower, W. John</creator><creator>Scott, John M.</creator><creator>Weir, Donald G.</creator><creator>Molloy, Anne M.</creator><creator>Kennedy, Seamus</creator><creator>Young, Paul B.</creator><general>Elsevier Inc</general><general>American Society for Nutritional Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920701</creationdate><title>Cobalt-Vitamin B-12 Deficiency Decreases Methionine Synthase Activity and Phospholipid Methylation in Sheep</title><author>Kennedy, D. Glenn ; Blanchflower, W. John ; Scott, John M. ; Weir, Donald G. ; Molloy, Anne M. ; Kennedy, Seamus ; Young, Paul B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c489t-fca4515c3256faee21e2941d1e0523f5b5d5c7664aba6a447b3f66a23a918ba23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase - metabolism</topic><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brain - enzymology</topic><topic>Brain - metabolism</topic><topic>CARENCE EN VITAMINE</topic><topic>CARENCE MINERALE</topic><topic>COBALT</topic><topic>Cobalt - deficiency</topic><topic>COBALTO</topic><topic>DEFICIENCIA DE MINERALES</topic><topic>DEFICIENCIA DE VITAMINAS</topic><topic>DIETA</topic><topic>FOSFOLIPIDOS</topic><topic>hypomethylation</topic><topic>Kidney - enzymology</topic><topic>Kidney - metabolism</topic><topic>LIGASAS</topic><topic>LIGASE</topic><topic>Liver - enzymology</topic><topic>Liver - metabolism</topic><topic>Medical sciences</topic><topic>Metabolic diseases</topic><topic>METHIONINE</topic><topic>METHYLATION</topic><topic>METILACION</topic><topic>METIONINA</topic><topic>Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...)</topic><topic>OVIN</topic><topic>OVINOS</topic><topic>PHOSPHATIDE</topic><topic>phosphatidyl choline</topic><topic>Phosphatidylcholines - metabolism</topic><topic>Phosphatidylethanolamines - metabolism</topic><topic>REGIME ALIMENTAIRE</topic><topic>Sheep</topic><topic>Vitamin B 12 Deficiency - enzymology</topic><topic>Vitamin B 12 Deficiency - metabolism</topic><topic>vitamin B-12</topic><topic>VITAMINA B12</topic><topic>VITAMINE B12</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kennedy, D. Glenn</creatorcontrib><creatorcontrib>Blanchflower, W. John</creatorcontrib><creatorcontrib>Scott, John M.</creatorcontrib><creatorcontrib>Weir, Donald G.</creatorcontrib><creatorcontrib>Molloy, Anne M.</creatorcontrib><creatorcontrib>Kennedy, Seamus</creatorcontrib><creatorcontrib>Young, Paul B.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kennedy, D. Glenn</au><au>Blanchflower, W. John</au><au>Scott, John M.</au><au>Weir, Donald G.</au><au>Molloy, Anne M.</au><au>Kennedy, Seamus</au><au>Young, Paul B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cobalt-Vitamin B-12 Deficiency Decreases Methionine Synthase Activity and Phospholipid Methylation in Sheep</atitle><jtitle>The Journal of nutrition</jtitle><addtitle>J Nutr</addtitle><date>1992-07-01</date><risdate>1992</risdate><volume>122</volume><issue>7</issue><spage>1384</spage><epage>1390</epage><pages>1384-1390</pages><issn>0022-3166</issn><eissn>1541-6100</eissn><coden>JONUAI</coden><abstract>Two groups of lambs were fed either a Co-deficient or a Co-sufficient whole barley-based diet for 28 wk to induce a severe Co-vitamin B-12 deficiency. Holo and apo methionine synthase activities were significantly lower in the liver, kidney and spinal cord of Co-deficient animals compared with controls. Neither form of this enzyme in the brain was affected by Co deficiency. The ratio of the tissue concentrations of S-adenosyl methionine to S-adenosyl homocysteine was significantly lower only in the liver of Co-deficient animals, suggesting that the activity of hepatic SAM-dependent methyltransferase enzymes would be impaired. Measurements of tissue concentrations of phosphatidyl choline and phosphatidyl ethanolamine revealed lower concentrations of phosphatidyl choline and a lower phosphatidyl choline:phosphatidyl ethanolamine ratio in both liver and brain of the Co-deficient animals. The latter finding occurred in the absence of changes in either methionine synthase activity or the methylation ratio and may result from impaired availability of hepatic phosphatidyl choline for transport into the brain.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>1619466</pmid><doi>10.1093/jn/122.7.1384</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0022-3166 |
| ispartof | The Journal of nutrition, 1992-07, Vol.122 (7), p.1384-1390 |
| issn | 0022-3166 1541-6100 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase - metabolism ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Animals Biological and medical sciences Brain - enzymology Brain - metabolism CARENCE EN VITAMINE CARENCE MINERALE COBALT Cobalt - deficiency COBALTO DEFICIENCIA DE MINERALES DEFICIENCIA DE VITAMINAS DIETA FOSFOLIPIDOS hypomethylation Kidney - enzymology Kidney - metabolism LIGASAS LIGASE Liver - enzymology Liver - metabolism Medical sciences Metabolic diseases METHIONINE METHYLATION METILACION METIONINA Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...) OVIN OVINOS PHOSPHATIDE phosphatidyl choline Phosphatidylcholines - metabolism Phosphatidylethanolamines - metabolism REGIME ALIMENTAIRE Sheep Vitamin B 12 Deficiency - enzymology Vitamin B 12 Deficiency - metabolism vitamin B-12 VITAMINA B12 VITAMINE B12 |
| title | Cobalt-Vitamin B-12 Deficiency Decreases Methionine Synthase Activity and Phospholipid Methylation in Sheep |
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