Isolation of cDNA clones encoding rat glial fibrillary acidic protein: Expression in astrocytes and in Schwann cells
Glial fibrillary acidic protein (GFAP) expressed by astrocytes in the central nervous system (CNS) has been extensively characterized but the molecular identity of related molecules in the peripheral nervous system (PNS) remains unclear. To examine possible structural differences between CNS and PNS...
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| Veröffentlicht in: | Journal of neuroscience research 1992-05, Vol.32 (1), p.1-14 |
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| description | Glial fibrillary acidic protein (GFAP) expressed by astrocytes in the central nervous system (CNS) has been extensively characterized but the molecular identity of related molecules in the peripheral nervous system (PNS) remains unclear. To examine possible structural differences between CNS and PNS GFAP, we have isolated cDNA clones for rat GFAP from both cultured astrocyte and Schwann cell libraries. Nucleotide sequence analysis indicated that the PNS and CNS GFAP clones contained identical coding regions, with a predicted protein product of 430 amino acids. However, the 5′‐untranslated region of clone rGFA15, isolated from the Schwann cell library, was longer than that predicted for brain‐derived GFAP mRNA. Primer extension analysis of RNA isolated from the RT4‐D6 Schwann cell line indicated that the start site for PNS GFAP mRNA lies 169 bases upstream from that used in the CNS. In addition, tryptic peptide mapping of GFAP prepared from cultured astrocytes and Schwann cells revealed one major peptide fragment present in CNS GFAP but absent from PNS GFAP. These results suggest structural differences between GFAP in these two cell types, at both the nucleic acid and protein level, and are consistent with previous observations of immunochemical differences existing between CNS and PNS GFAP. © 1992 Wiiey‐Liss, Inc. © 1992 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/jnr.490320102 |
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L. ; Weinmaster, G. A. ; Milner, R. J.</creator><creatorcontrib>Feinstein, D. L. ; Weinmaster, G. A. ; Milner, R. J.</creatorcontrib><description>Glial fibrillary acidic protein (GFAP) expressed by astrocytes in the central nervous system (CNS) has been extensively characterized but the molecular identity of related molecules in the peripheral nervous system (PNS) remains unclear. To examine possible structural differences between CNS and PNS GFAP, we have isolated cDNA clones for rat GFAP from both cultured astrocyte and Schwann cell libraries. Nucleotide sequence analysis indicated that the PNS and CNS GFAP clones contained identical coding regions, with a predicted protein product of 430 amino acids. However, the 5′‐untranslated region of clone rGFA15, isolated from the Schwann cell library, was longer than that predicted for brain‐derived GFAP mRNA. Primer extension analysis of RNA isolated from the RT4‐D6 Schwann cell line indicated that the start site for PNS GFAP mRNA lies 169 bases upstream from that used in the CNS. In addition, tryptic peptide mapping of GFAP prepared from cultured astrocytes and Schwann cells revealed one major peptide fragment present in CNS GFAP but absent from PNS GFAP. These results suggest structural differences between GFAP in these two cell types, at both the nucleic acid and protein level, and are consistent with previous observations of immunochemical differences existing between CNS and PNS GFAP. © 1992 Wiiey‐Liss, Inc. © 1992 Wiley‐Liss, Inc.</description><identifier>ISSN: 0360-4012</identifier><identifier>EISSN: 1097-4547</identifier><identifier>DOI: 10.1002/jnr.490320102</identifier><identifier>PMID: 1629938</identifier><identifier>CODEN: JNREDK</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Amino Acid Sequence ; Animals ; astrocytes ; Astrocytes - metabolism ; Base Sequence ; Biological and medical sciences ; cDNA ; Cells, Cultured ; Cloning, Molecular ; DNA - isolation & purification ; Fundamental and applied biological sciences. Psychology ; Gene Library ; genes ; Glial Fibrillary Acidic Protein - genetics ; Intermediate filament ; Isolated neuron and nerve. Neuroglia ; Molecular Sequence Data ; mRNA ; Oligonucleotide Probes - genetics ; Peptide Mapping ; Rats ; RNA, Messenger - isolation & purification ; Schwann cells ; Schwann Cells - metabolism ; transcription initiation ; Transcription, Genetic ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of neuroscience research, 1992-05, Vol.32 (1), p.1-14</ispartof><rights>Copyright © 1992 Wiley‐Liss, Inc.</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5002-d7cbc4ab7e4b0909363cfdc98709c787186c775b580864a22f2c2cf8a54817f43</citedby><cites>FETCH-LOGICAL-c5002-d7cbc4ab7e4b0909363cfdc98709c787186c775b580864a22f2c2cf8a54817f43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjnr.490320102$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjnr.490320102$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5304441$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1629938$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Feinstein, D. L.</creatorcontrib><creatorcontrib>Weinmaster, G. A.</creatorcontrib><creatorcontrib>Milner, R. J.</creatorcontrib><title>Isolation of cDNA clones encoding rat glial fibrillary acidic protein: Expression in astrocytes and in Schwann cells</title><title>Journal of neuroscience research</title><addtitle>J. Neurosci. Res</addtitle><description>Glial fibrillary acidic protein (GFAP) expressed by astrocytes in the central nervous system (CNS) has been extensively characterized but the molecular identity of related molecules in the peripheral nervous system (PNS) remains unclear. To examine possible structural differences between CNS and PNS GFAP, we have isolated cDNA clones for rat GFAP from both cultured astrocyte and Schwann cell libraries. Nucleotide sequence analysis indicated that the PNS and CNS GFAP clones contained identical coding regions, with a predicted protein product of 430 amino acids. However, the 5′‐untranslated region of clone rGFA15, isolated from the Schwann cell library, was longer than that predicted for brain‐derived GFAP mRNA. Primer extension analysis of RNA isolated from the RT4‐D6 Schwann cell line indicated that the start site for PNS GFAP mRNA lies 169 bases upstream from that used in the CNS. In addition, tryptic peptide mapping of GFAP prepared from cultured astrocytes and Schwann cells revealed one major peptide fragment present in CNS GFAP but absent from PNS GFAP. These results suggest structural differences between GFAP in these two cell types, at both the nucleic acid and protein level, and are consistent with previous observations of immunochemical differences existing between CNS and PNS GFAP. © 1992 Wiiey‐Liss, Inc. © 1992 Wiley‐Liss, Inc.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>astrocytes</subject><subject>Astrocytes - metabolism</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>cDNA</subject><subject>Cells, Cultured</subject><subject>Cloning, Molecular</subject><subject>DNA - isolation & purification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Library</subject><subject>genes</subject><subject>Glial Fibrillary Acidic Protein - genetics</subject><subject>Intermediate filament</subject><subject>Isolated neuron and nerve. Neuroglia</subject><subject>Molecular Sequence Data</subject><subject>mRNA</subject><subject>Oligonucleotide Probes - genetics</subject><subject>Peptide Mapping</subject><subject>Rats</subject><subject>RNA, Messenger - isolation & purification</subject><subject>Schwann cells</subject><subject>Schwann Cells - metabolism</subject><subject>transcription initiation</subject><subject>Transcription, Genetic</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0360-4012</issn><issn>1097-4547</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1vEzEQxS1EVULLkSOSD4jblvHau7a5VaWUVlWQKB8SF8s76y0ujjfYG7X573GUKOUEp5Fmfn7zPI-QlwxOGED99i6mE6GB18CgfkJmDLSsRCPkUzID3kIlgNXPyPOc7wBA64YfkkPW1lpzNSPTZR6DnfwY6ThQfD8_pRjG6DJ1Ecfex1ua7ERvg7eBDr5LPgSb1tSi7z3SZRon5-M7ev6wTC7njY6P1OYpjbieioyN_aZzgz_vbYwUXQj5mBwMNmT3YlePyNcP51_OPlbXny4uz06vK2zKz6peYofCdtKJDjRo3nIcetRKgkapJFMtStl0jQLVClvXQ401Dso2QjE5CH5E3mx1i83fK5cns_B548BGN66ykRxEqzj_L8jasqsGVcBqC2Iac05uMMvkF-UghoHZxGFKHGYfR-Ff7YRX3cL1j_T2_mX-eje3GW0Yko3o8x5rikEhWMHkFrv3wa3_vdNczT__bWBn2OfJPexf2vTLtJLLxnyfXxjG1Df5Q18Zzv8A3wGxwA</recordid><startdate>199205</startdate><enddate>199205</enddate><creator>Feinstein, D. L.</creator><creator>Weinmaster, G. A.</creator><creator>Milner, R. J.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199205</creationdate><title>Isolation of cDNA clones encoding rat glial fibrillary acidic protein: Expression in astrocytes and in Schwann cells</title><author>Feinstein, D. L. ; Weinmaster, G. A. ; Milner, R. J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5002-d7cbc4ab7e4b0909363cfdc98709c787186c775b580864a22f2c2cf8a54817f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>astrocytes</topic><topic>Astrocytes - metabolism</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>cDNA</topic><topic>Cells, Cultured</topic><topic>Cloning, Molecular</topic><topic>DNA - isolation & purification</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Library</topic><topic>genes</topic><topic>Glial Fibrillary Acidic Protein - genetics</topic><topic>Intermediate filament</topic><topic>Isolated neuron and nerve. Neuroglia</topic><topic>Molecular Sequence Data</topic><topic>mRNA</topic><topic>Oligonucleotide Probes - genetics</topic><topic>Peptide Mapping</topic><topic>Rats</topic><topic>RNA, Messenger - isolation & purification</topic><topic>Schwann cells</topic><topic>Schwann Cells - metabolism</topic><topic>transcription initiation</topic><topic>Transcription, Genetic</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Feinstein, D. L.</creatorcontrib><creatorcontrib>Weinmaster, G. A.</creatorcontrib><creatorcontrib>Milner, R. J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroscience research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Feinstein, D. L.</au><au>Weinmaster, G. A.</au><au>Milner, R. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of cDNA clones encoding rat glial fibrillary acidic protein: Expression in astrocytes and in Schwann cells</atitle><jtitle>Journal of neuroscience research</jtitle><addtitle>J. Neurosci. Res</addtitle><date>1992-05</date><risdate>1992</risdate><volume>32</volume><issue>1</issue><spage>1</spage><epage>14</epage><pages>1-14</pages><issn>0360-4012</issn><eissn>1097-4547</eissn><coden>JNREDK</coden><abstract>Glial fibrillary acidic protein (GFAP) expressed by astrocytes in the central nervous system (CNS) has been extensively characterized but the molecular identity of related molecules in the peripheral nervous system (PNS) remains unclear. To examine possible structural differences between CNS and PNS GFAP, we have isolated cDNA clones for rat GFAP from both cultured astrocyte and Schwann cell libraries. Nucleotide sequence analysis indicated that the PNS and CNS GFAP clones contained identical coding regions, with a predicted protein product of 430 amino acids. However, the 5′‐untranslated region of clone rGFA15, isolated from the Schwann cell library, was longer than that predicted for brain‐derived GFAP mRNA. Primer extension analysis of RNA isolated from the RT4‐D6 Schwann cell line indicated that the start site for PNS GFAP mRNA lies 169 bases upstream from that used in the CNS. In addition, tryptic peptide mapping of GFAP prepared from cultured astrocytes and Schwann cells revealed one major peptide fragment present in CNS GFAP but absent from PNS GFAP. These results suggest structural differences between GFAP in these two cell types, at both the nucleic acid and protein level, and are consistent with previous observations of immunochemical differences existing between CNS and PNS GFAP. © 1992 Wiiey‐Liss, Inc. © 1992 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>1629938</pmid><doi>10.1002/jnr.490320102</doi><tpages>14</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals astrocytes Astrocytes - metabolism Base Sequence Biological and medical sciences cDNA Cells, Cultured Cloning, Molecular DNA - isolation & purification Fundamental and applied biological sciences. Psychology Gene Library genes Glial Fibrillary Acidic Protein - genetics Intermediate filament Isolated neuron and nerve. Neuroglia Molecular Sequence Data mRNA Oligonucleotide Probes - genetics Peptide Mapping Rats RNA, Messenger - isolation & purification Schwann cells Schwann Cells - metabolism transcription initiation Transcription, Genetic Vertebrates: nervous system and sense organs |
| title | Isolation of cDNA clones encoding rat glial fibrillary acidic protein: Expression in astrocytes and in Schwann cells |
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