Cutting Edge: Molecular Cloning of a Killer Cell Ig-Like Receptor in the Mouse and Rat
We report the molecular cloning of a KIR3DL1 receptor in the mouse and the rat, between 37.4 and 45.4% identical with primate killer cell Ig-like receptors (KIRs/CD158). Both mouse and rat molecules contain a pair of immunoreceptor tyrosine-based inhibition motifs in their cytoplasmic regions, sugge...
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| Veröffentlicht in: | The Journal of immunology (1950) 2003-03, Vol.170 (5), p.2259-2263 |
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| container_title | The Journal of immunology (1950) |
| container_volume | 170 |
| creator | Hoelsbrekken, Sigurd E Nylenna, Oyvind Saether, Per C Slettedal, Imer O Ryan, James C Fossum, Sigbjorn Dissen, Erik |
| description | We report the molecular cloning of a KIR3DL1 receptor in the mouse and the rat, between 37.4 and 45.4% identical with primate killer cell Ig-like receptors (KIRs/CD158). Both mouse and rat molecules contain a pair of immunoreceptor tyrosine-based inhibition motifs in their cytoplasmic regions, suggesting an inhibitory function. Southern blot analysis indicated a single KIR gene in the rat, whereas the mouse genome contains more than one KIR-related element. The rat Kir3dl1 locus was mapped to the leukocyte receptor gene complex on chromosome 1, whereas mouse Kir3dl1 was localized to the X chromosome. RT-PCR demonstrated that KIR3DL1 was selectively expressed by NK cells in both rat and mouse. An epitope-tagged expression construct of mouse KIR3DL1 transfected into 293T cells induced expression of a approximately 55-kDa protein. Our data indicate that KIR receptors may contribute to the NK cell receptor repertoire in rodents, alongside the Ly-49 family. |
| doi_str_mv | 10.4049/jimmunol.170.5.2259 |
| format | Article |
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Both mouse and rat molecules contain a pair of immunoreceptor tyrosine-based inhibition motifs in their cytoplasmic regions, suggesting an inhibitory function. Southern blot analysis indicated a single KIR gene in the rat, whereas the mouse genome contains more than one KIR-related element. The rat Kir3dl1 locus was mapped to the leukocyte receptor gene complex on chromosome 1, whereas mouse Kir3dl1 was localized to the X chromosome. RT-PCR demonstrated that KIR3DL1 was selectively expressed by NK cells in both rat and mouse. An epitope-tagged expression construct of mouse KIR3DL1 transfected into 293T cells induced expression of a approximately 55-kDa protein. Our data indicate that KIR receptors may contribute to the NK cell receptor repertoire in rodents, alongside the Ly-49 family.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.170.5.2259</identifier><identifier>PMID: 12594244</identifier><language>eng</language><publisher>United States: Am Assoc Immnol</publisher><subject>Amino Acid Sequence ; Animals ; Cell Line ; Chromosome Mapping ; Cloning, Molecular ; Cricetinae ; DNA, Complementary - isolation & purification ; Humans ; Killer Cells, Natural - immunology ; Killer Cells, Natural - metabolism ; Membrane Proteins - biosynthesis ; Membrane Proteins - genetics ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Molecular Sequence Data ; Rats ; Receptors, Immunologic - biosynthesis ; Receptors, Immunologic - chemistry ; Receptors, Immunologic - genetics ; Receptors, KIR ; Receptors, KIR3DL1 ; Sequence Homology, Amino Acid ; Transcription, Genetic - immunology ; Transfection</subject><ispartof>The Journal of immunology (1950), 2003-03, Vol.170 (5), p.2259-2263</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-793c17a562f022739c89b930a05421b50635cfe5d75d0754d8aecb064d620f013</citedby><cites>FETCH-LOGICAL-c444t-793c17a562f022739c89b930a05421b50635cfe5d75d0754d8aecb064d620f013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12594244$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hoelsbrekken, Sigurd E</creatorcontrib><creatorcontrib>Nylenna, Oyvind</creatorcontrib><creatorcontrib>Saether, Per C</creatorcontrib><creatorcontrib>Slettedal, Imer O</creatorcontrib><creatorcontrib>Ryan, James C</creatorcontrib><creatorcontrib>Fossum, Sigbjorn</creatorcontrib><creatorcontrib>Dissen, Erik</creatorcontrib><title>Cutting Edge: Molecular Cloning of a Killer Cell Ig-Like Receptor in the Mouse and Rat</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>We report the molecular cloning of a KIR3DL1 receptor in the mouse and the rat, between 37.4 and 45.4% identical with primate killer cell Ig-like receptors (KIRs/CD158). Both mouse and rat molecules contain a pair of immunoreceptor tyrosine-based inhibition motifs in their cytoplasmic regions, suggesting an inhibitory function. Southern blot analysis indicated a single KIR gene in the rat, whereas the mouse genome contains more than one KIR-related element. The rat Kir3dl1 locus was mapped to the leukocyte receptor gene complex on chromosome 1, whereas mouse Kir3dl1 was localized to the X chromosome. RT-PCR demonstrated that KIR3DL1 was selectively expressed by NK cells in both rat and mouse. An epitope-tagged expression construct of mouse KIR3DL1 transfected into 293T cells induced expression of a approximately 55-kDa protein. Our data indicate that KIR receptors may contribute to the NK cell receptor repertoire in rodents, alongside the Ly-49 family.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Cell Line</subject><subject>Chromosome Mapping</subject><subject>Cloning, Molecular</subject><subject>Cricetinae</subject><subject>DNA, Complementary - isolation & purification</subject><subject>Humans</subject><subject>Killer Cells, Natural - immunology</subject><subject>Killer Cells, Natural - metabolism</subject><subject>Membrane Proteins - biosynthesis</subject><subject>Membrane Proteins - genetics</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular Sequence Data</subject><subject>Rats</subject><subject>Receptors, Immunologic - biosynthesis</subject><subject>Receptors, Immunologic - chemistry</subject><subject>Receptors, Immunologic - genetics</subject><subject>Receptors, KIR</subject><subject>Receptors, KIR3DL1</subject><subject>Sequence Homology, Amino Acid</subject><subject>Transcription, Genetic - immunology</subject><subject>Transfection</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkF1LKzEURYMo2qv-AkHypE9TTzL5cHyTUr1iRRD1NaSZM200M1MnMxT_vSntxft04LD2ZrMIOWMwFiCKqw9f10PThjHTMJZjzmWxR0ZMSsiUArVPRgCcZ0wrfUT-xPgBAAq4OCRHLLGCCzEi75Oh732zoNNygTf0qQ3ohmA7Oglts_m3FbX00YeA6Ych0IdFNvOfSF_Q4apvO-ob2i8xRYeI1DYlfbH9CTmobIh4urvH5O1u-jr5m82e7x8mt7PMCSH6TBe5Y9pKxas0VeeFuy7mRQ4WpOBsLkHl0lUoSy1L0FKU1xbdHJQoFYcKWH5MLra9q679GjD2pvbRpZm2wbTH6BxErhgkMN-Crmtj7LAyq87Xtvs2DMxGp_mn0ySdRpqNzpQ639UP8xrL38zOXwIut8DSL5Zr36GJtQ0h4cys1-v_qn4AKHN-Hw</recordid><startdate>20030301</startdate><enddate>20030301</enddate><creator>Hoelsbrekken, Sigurd E</creator><creator>Nylenna, Oyvind</creator><creator>Saether, Per C</creator><creator>Slettedal, Imer O</creator><creator>Ryan, James C</creator><creator>Fossum, Sigbjorn</creator><creator>Dissen, Erik</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030301</creationdate><title>Cutting Edge: Molecular Cloning of a Killer Cell Ig-Like Receptor in the Mouse and Rat</title><author>Hoelsbrekken, Sigurd E ; Nylenna, Oyvind ; Saether, Per C ; Slettedal, Imer O ; Ryan, James C ; Fossum, Sigbjorn ; Dissen, Erik</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-793c17a562f022739c89b930a05421b50635cfe5d75d0754d8aecb064d620f013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Cell Line</topic><topic>Chromosome Mapping</topic><topic>Cloning, Molecular</topic><topic>Cricetinae</topic><topic>DNA, Complementary - isolation & purification</topic><topic>Humans</topic><topic>Killer Cells, Natural - immunology</topic><topic>Killer Cells, Natural - metabolism</topic><topic>Membrane Proteins - biosynthesis</topic><topic>Membrane Proteins - genetics</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Molecular Sequence Data</topic><topic>Rats</topic><topic>Receptors, Immunologic - biosynthesis</topic><topic>Receptors, Immunologic - chemistry</topic><topic>Receptors, Immunologic - genetics</topic><topic>Receptors, KIR</topic><topic>Receptors, KIR3DL1</topic><topic>Sequence Homology, Amino Acid</topic><topic>Transcription, Genetic - immunology</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hoelsbrekken, Sigurd E</creatorcontrib><creatorcontrib>Nylenna, Oyvind</creatorcontrib><creatorcontrib>Saether, Per C</creatorcontrib><creatorcontrib>Slettedal, Imer O</creatorcontrib><creatorcontrib>Ryan, James C</creatorcontrib><creatorcontrib>Fossum, Sigbjorn</creatorcontrib><creatorcontrib>Dissen, Erik</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hoelsbrekken, Sigurd E</au><au>Nylenna, Oyvind</au><au>Saether, Per C</au><au>Slettedal, Imer O</au><au>Ryan, James C</au><au>Fossum, Sigbjorn</au><au>Dissen, Erik</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cutting Edge: Molecular Cloning of a Killer Cell Ig-Like Receptor in the Mouse and Rat</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>2003-03-01</date><risdate>2003</risdate><volume>170</volume><issue>5</issue><spage>2259</spage><epage>2263</epage><pages>2259-2263</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>We report the molecular cloning of a KIR3DL1 receptor in the mouse and the rat, between 37.4 and 45.4% identical with primate killer cell Ig-like receptors (KIRs/CD158). Both mouse and rat molecules contain a pair of immunoreceptor tyrosine-based inhibition motifs in their cytoplasmic regions, suggesting an inhibitory function. Southern blot analysis indicated a single KIR gene in the rat, whereas the mouse genome contains more than one KIR-related element. The rat Kir3dl1 locus was mapped to the leukocyte receptor gene complex on chromosome 1, whereas mouse Kir3dl1 was localized to the X chromosome. RT-PCR demonstrated that KIR3DL1 was selectively expressed by NK cells in both rat and mouse. An epitope-tagged expression construct of mouse KIR3DL1 transfected into 293T cells induced expression of a approximately 55-kDa protein. Our data indicate that KIR receptors may contribute to the NK cell receptor repertoire in rodents, alongside the Ly-49 family.</abstract><cop>United States</cop><pub>Am Assoc Immnol</pub><pmid>12594244</pmid><doi>10.4049/jimmunol.170.5.2259</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Cell Line Chromosome Mapping Cloning, Molecular Cricetinae DNA, Complementary - isolation & purification Humans Killer Cells, Natural - immunology Killer Cells, Natural - metabolism Membrane Proteins - biosynthesis Membrane Proteins - genetics Mice Mice, Inbred BALB C Mice, Inbred C57BL Molecular Sequence Data Rats Receptors, Immunologic - biosynthesis Receptors, Immunologic - chemistry Receptors, Immunologic - genetics Receptors, KIR Receptors, KIR3DL1 Sequence Homology, Amino Acid Transcription, Genetic - immunology Transfection |
| title | Cutting Edge: Molecular Cloning of a Killer Cell Ig-Like Receptor in the Mouse and Rat |
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