Porcine conceptus proteins: antiviral activity and effect on 2',5'-oligoadenylate synthetase

Interferon-like proteins synthesized by conceptuses of domestic ruminants inhibit luteolysis during early pregnancy. Although pig conceptuses secrete trophoblast interferons during the period of CL maintenance, estrogen is involved with maintenance of the CL. The principal purposes of this work were...

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Veröffentlicht in:Biology of reproduction 1992-03, Vol.46 (3), p.464-469
Hauptverfasser: Short, E.C. Jr. (Oklahoma State University, Stillwater, OK), Geisert, R.D, Groothuis, P.G, Blair, R.M, Schmitt, R.A.M, Fulton, R.W
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container_end_page 469
container_issue 3
container_start_page 464
container_title Biology of reproduction
container_volume 46
creator Short, E.C. Jr. (Oklahoma State University, Stillwater, OK)
Geisert, R.D
Groothuis, P.G
Blair, R.M
Schmitt, R.A.M
Fulton, R.W
description Interferon-like proteins synthesized by conceptuses of domestic ruminants inhibit luteolysis during early pregnancy. Although pig conceptuses secrete trophoblast interferons during the period of CL maintenance, estrogen is involved with maintenance of the CL. The principal purposes of this work were to confirm production of trophoblast interferons by porcine conceptuses and to compare the effect of trophoblast interferons on endometrium of pigs and cattle. When measured using Madin-Darby bovine kidney (MDBK) cells challenged with vesicular stomatitis virus, antiviral activity in uterine flushings from cyclic gilts was not detectable throughout the estrous cycle; however, in pregnant gilts, antiviral activity increased from undetectable amounts to 4-11 X 10(4) U on Days 14, 16, and 18. Porcine embryos in culture produced 1,100 U/embryo/ml/24 h. Porcine conceptus secretory proteins induced 2',5'-oligo(A) synthetase in MDBK cells and in endometrial explants of cows but had no measurable effect on 2',5'-oligo(A) synthetase activity of endometrial explants of pigs. Similarly, endometrial 2',5'-oligo(A) synthetase of pregnant pigs was unaffected in vivo during the period of maximal synthesis of conceptus secretory proteins. Porcine conceptus secretory proteins produced no detectable increase in serum antiviral activity or 2',5'-oligo(A) synthetase activity of blood mononuclear leukocytes in utero-ovarian venous blood. These results suggest that conceptus interferons of pigs play different roles in the establishment of pregnancy compared to their roles in ruminants
doi_str_mv 10.1095/biolreprod46.3.464
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When measured using Madin-Darby bovine kidney (MDBK) cells challenged with vesicular stomatitis virus, antiviral activity in uterine flushings from cyclic gilts was not detectable throughout the estrous cycle; however, in pregnant gilts, antiviral activity increased from undetectable amounts to 4-11 X 10(4) U on Days 14, 16, and 18. Porcine embryos in culture produced 1,100 U/embryo/ml/24 h. Porcine conceptus secretory proteins induced 2',5'-oligo(A) synthetase in MDBK cells and in endometrial explants of cows but had no measurable effect on 2',5'-oligo(A) synthetase activity of endometrial explants of pigs. Similarly, endometrial 2',5'-oligo(A) synthetase of pregnant pigs was unaffected in vivo during the period of maximal synthesis of conceptus secretory proteins. Porcine conceptus secretory proteins produced no detectable increase in serum antiviral activity or 2',5'-oligo(A) synthetase activity of blood mononuclear leukocytes in utero-ovarian venous blood. 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The principal purposes of this work were to confirm production of trophoblast interferons by porcine conceptuses and to compare the effect of trophoblast interferons on endometrium of pigs and cattle. When measured using Madin-Darby bovine kidney (MDBK) cells challenged with vesicular stomatitis virus, antiviral activity in uterine flushings from cyclic gilts was not detectable throughout the estrous cycle; however, in pregnant gilts, antiviral activity increased from undetectable amounts to 4-11 X 10(4) U on Days 14, 16, and 18. Porcine embryos in culture produced 1,100 U/embryo/ml/24 h. Porcine conceptus secretory proteins induced 2',5'-oligo(A) synthetase in MDBK cells and in endometrial explants of cows but had no measurable effect on 2',5'-oligo(A) synthetase activity of endometrial explants of pigs. Similarly, endometrial 2',5'-oligo(A) synthetase of pregnant pigs was unaffected in vivo during the period of maximal synthesis of conceptus secretory proteins. Porcine conceptus secretory proteins produced no detectable increase in serum antiviral activity or 2',5'-oligo(A) synthetase activity of blood mononuclear leukocytes in utero-ovarian venous blood. These results suggest that conceptus interferons of pigs play different roles in the establishment of pregnancy compared to their roles in ruminants</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>1319754</pmid><doi>10.1095/biolreprod46.3.464</doi><tpages>6</tpages></addata></record>
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subjects 2',5'-Oligoadenylate Synthetase - metabolism
Animals
Antiviral Agents - pharmacology
Biological and medical sciences
Cattle
Cell Line
CERDO
Culture Techniques
Endometrium - enzymology
Female
Fundamental and applied biological sciences. Psychology
Hormone metabolism and regulation
Interferon Type I - biosynthesis
Interferon Type I - pharmacology
Leukocytes, Mononuclear - drug effects
Leukocytes, Mononuclear - enzymology
Mammalian female genital system
PORCIN
Pregnancy
Pregnancy Proteins - biosynthesis
Pregnancy Proteins - pharmacology
Pregnancy, Animal - metabolism
PROPIEDADES ANTIMICROBIANAS
PROPRIETE ANTIMICROBIENNE
PROTEINAS
PROTEINE
Swine - embryology
Swine - metabolism
Uterus - microbiology
Vertebrates: reproduction
Vesicular stomatitis Indiana virus - drug effects
title Porcine conceptus proteins: antiviral activity and effect on 2',5'-oligoadenylate synthetase
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