The pattern of joining (JH) gene usage in the human IgH chain is established predominantly at the B precursor cell stage
Preferential utilization of JH and D genes has been demonstrated in the rearranged IgH chain in human peripheral B cells. We report here that the same hierarchy of JH gene usage is observed in leukemic cells arrested in the B precursor stage of differentiation. Specifically, JH4 and JH6 accounted fo...
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| Veröffentlicht in: | The Journal of immunology (1950) 1992-07, Vol.149 (2), p.511-516 |
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| creator | Wasserman, R Ito, Y Galili, N Yamada, M Reichard, BA Shane, S Lange, B Rovera, G |
| description | Preferential utilization of JH and D genes has been demonstrated in the rearranged IgH chain in human peripheral B cells. We report here that the same hierarchy of JH gene usage is observed in leukemic cells arrested in the B precursor stage of differentiation. Specifically, JH4 and JH6 accounted for 42.9% and 35.7%, respectively, of the JH gene usage in the leukemias compared with an expected frequency of 16.7% assuming unbiased gene usage. Within the D gene families, the DN1 gene appears to be overutilized in both populations, representing about 15% of the total gene usage compared with an expected frequency of 3.2%. Because 21 of the 36 leukemias contained only nonproductive IgH rearrangements, the preferential gene usage could not have arisen from pre-B cells that have undergone clonal selection after a productive rearrangement but before surface Ig expression. Nonproductive rearrangements exhibited the biased gene usage seen for productive rearrangements. These findings suggest that a recombination bias favoring certain segments may be the actual mechanism responsible for the apparent preferential utilization of JH and D genes. |
| doi_str_mv | 10.4049/jimmunol.149.2.511 |
| format | Article |
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We report here that the same hierarchy of JH gene usage is observed in leukemic cells arrested in the B precursor stage of differentiation. Specifically, JH4 and JH6 accounted for 42.9% and 35.7%, respectively, of the JH gene usage in the leukemias compared with an expected frequency of 16.7% assuming unbiased gene usage. Within the D gene families, the DN1 gene appears to be overutilized in both populations, representing about 15% of the total gene usage compared with an expected frequency of 3.2%. Because 21 of the 36 leukemias contained only nonproductive IgH rearrangements, the preferential gene usage could not have arisen from pre-B cells that have undergone clonal selection after a productive rearrangement but before surface Ig expression. Nonproductive rearrangements exhibited the biased gene usage seen for productive rearrangements. These findings suggest that a recombination bias favoring certain segments may be the actual mechanism responsible for the apparent preferential utilization of JH and D genes.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.149.2.511</identifier><identifier>PMID: 1624797</identifier><identifier>CODEN: JOIMA3</identifier><language>eng</language><publisher>Bethesda, MD: Am Assoc Immnol</publisher><subject>Adolescent ; B-Lymphocytes - immunology ; Base Sequence ; Biological and medical sciences ; Cells, Cultured ; Child ; Child, Preschool ; Fundamental and applied biological sciences. Psychology ; Gene Rearrangement ; Genes, Immunoglobulin ; Genic rearrangement. Recombination. 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We report here that the same hierarchy of JH gene usage is observed in leukemic cells arrested in the B precursor stage of differentiation. Specifically, JH4 and JH6 accounted for 42.9% and 35.7%, respectively, of the JH gene usage in the leukemias compared with an expected frequency of 16.7% assuming unbiased gene usage. Within the D gene families, the DN1 gene appears to be overutilized in both populations, representing about 15% of the total gene usage compared with an expected frequency of 3.2%. Because 21 of the 36 leukemias contained only nonproductive IgH rearrangements, the preferential gene usage could not have arisen from pre-B cells that have undergone clonal selection after a productive rearrangement but before surface Ig expression. Nonproductive rearrangements exhibited the biased gene usage seen for productive rearrangements. These findings suggest that a recombination bias favoring certain segments may be the actual mechanism responsible for the apparent preferential utilization of JH and D genes.</description><subject>Adolescent</subject><subject>B-Lymphocytes - immunology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Rearrangement</subject><subject>Genes, Immunoglobulin</subject><subject>Genic rearrangement. Recombination. Transposable element</subject><subject>Hematopoietic Stem Cells - immunology</subject><subject>Humans</subject><subject>Immunoglobulin Heavy Chains - genetics</subject><subject>Immunoglobulin Joining Region - genetics</subject><subject>Immunoglobulin Variable Region - genetics</subject><subject>Infant</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS1EVZbCF0BC8gEhOGSxHf9JjqUCtlUlLuVsOc4k8cpxFjvR0m-Pl126x55Gmvm9N096CL2jZM0Jr79s3TguYfJryus1WwtKX6AVFYIUUhL5Eq0IYaygSqpX6HVKW0KIJIxfoksqGVe1WqE_DwPgnZlniAFPHd5OLrjQ4093m8-4hwB4SaYH7AKeMzksown4tt9gO5i8cwlDmk3jXRqgxbsI7TS6YMLsH7GZ_2m-HtZ2iWmK2IL3OAt6eIMuOuMTvD3NK_Tr-7eHm01x__PH7c31fWF5Wc4FV8IaVVtqmBDMdI2AphVdJ6QsQTHR8LpsOVUVERW1VWUUbShta6grqGrByyv08ei7i9PvJYfVo0uHGCbAtCStSlIqQeWzIJU8_6xFBtkRtHFKKUKnd9GNJj5qSvShF_2_F5170UznXrLo_cl9aUZoz5JjEfn-4XQ3yRrfRROsS0-Y4JKUpTiHHFw_7F0EnUbjfTaler_fn__9BSNHpLA</recordid><startdate>19920715</startdate><enddate>19920715</enddate><creator>Wasserman, R</creator><creator>Ito, Y</creator><creator>Galili, N</creator><creator>Yamada, M</creator><creator>Reichard, BA</creator><creator>Shane, S</creator><creator>Lange, B</creator><creator>Rovera, G</creator><general>Am Assoc Immnol</general><general>American Association of Immunologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19920715</creationdate><title>The pattern of joining (JH) gene usage in the human IgH chain is established predominantly at the B precursor cell stage</title><author>Wasserman, R ; Ito, Y ; Galili, N ; Yamada, M ; Reichard, BA ; Shane, S ; Lange, B ; Rovera, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-475ca79c1a2552afb5ebd5ff5663e725b493d41780581c88a71b11d9e98e89543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Adolescent</topic><topic>B-Lymphocytes - immunology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Rearrangement</topic><topic>Genes, Immunoglobulin</topic><topic>Genic rearrangement. Recombination. Transposable element</topic><topic>Hematopoietic Stem Cells - immunology</topic><topic>Humans</topic><topic>Immunoglobulin Heavy Chains - genetics</topic><topic>Immunoglobulin Joining Region - genetics</topic><topic>Immunoglobulin Variable Region - genetics</topic><topic>Infant</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wasserman, R</creatorcontrib><creatorcontrib>Ito, Y</creatorcontrib><creatorcontrib>Galili, N</creatorcontrib><creatorcontrib>Yamada, M</creatorcontrib><creatorcontrib>Reichard, BA</creatorcontrib><creatorcontrib>Shane, S</creatorcontrib><creatorcontrib>Lange, B</creatorcontrib><creatorcontrib>Rovera, G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wasserman, R</au><au>Ito, Y</au><au>Galili, N</au><au>Yamada, M</au><au>Reichard, BA</au><au>Shane, S</au><au>Lange, B</au><au>Rovera, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The pattern of joining (JH) gene usage in the human IgH chain is established predominantly at the B precursor cell stage</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1992-07-15</date><risdate>1992</risdate><volume>149</volume><issue>2</issue><spage>511</spage><epage>516</epage><pages>511-516</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><coden>JOIMA3</coden><abstract>Preferential utilization of JH and D genes has been demonstrated in the rearranged IgH chain in human peripheral B cells. We report here that the same hierarchy of JH gene usage is observed in leukemic cells arrested in the B precursor stage of differentiation. Specifically, JH4 and JH6 accounted for 42.9% and 35.7%, respectively, of the JH gene usage in the leukemias compared with an expected frequency of 16.7% assuming unbiased gene usage. Within the D gene families, the DN1 gene appears to be overutilized in both populations, representing about 15% of the total gene usage compared with an expected frequency of 3.2%. Because 21 of the 36 leukemias contained only nonproductive IgH rearrangements, the preferential gene usage could not have arisen from pre-B cells that have undergone clonal selection after a productive rearrangement but before surface Ig expression. Nonproductive rearrangements exhibited the biased gene usage seen for productive rearrangements. These findings suggest that a recombination bias favoring certain segments may be the actual mechanism responsible for the apparent preferential utilization of JH and D genes.</abstract><cop>Bethesda, MD</cop><pub>Am Assoc Immnol</pub><pmid>1624797</pmid><doi>10.4049/jimmunol.149.2.511</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent B-Lymphocytes - immunology Base Sequence Biological and medical sciences Cells, Cultured Child Child, Preschool Fundamental and applied biological sciences. Psychology Gene Rearrangement Genes, Immunoglobulin Genic rearrangement. Recombination. Transposable element Hematopoietic Stem Cells - immunology Humans Immunoglobulin Heavy Chains - genetics Immunoglobulin Joining Region - genetics Immunoglobulin Variable Region - genetics Infant Molecular and cellular biology Molecular genetics Molecular Sequence Data |
| title | The pattern of joining (JH) gene usage in the human IgH chain is established predominantly at the B precursor cell stage |
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