Short communication: Point mutations in the dihydrofolate reductase and dihydropteroate synthase genes of Plasmodium falciparum isolates from Colombia
Summary Point mutations in the dihydrofolate reductase (DHFR) and dihydropteroate synthase (DHPS) genes of Plasmodium falciparum can lead to an increasing resistance of P. falciparum to pyrimethamine/sulphadoxine. We examined the prevalence of these mutations in 36 samples from Colombia. Analysed by...
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| Veröffentlicht in: | Tropical medicine & international health 2003-02, Vol.8 (2), p.129-132 |
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| creator | Schmider, Nadja Peyerl‐Hoffmann, Gaby Restrepo, Marcos Jelinek, Tomas |
| description | Summary
Point mutations in the dihydrofolate reductase (DHFR) and dihydropteroate synthase (DHPS) genes of Plasmodium falciparum can lead to an increasing resistance of P. falciparum to pyrimethamine/sulphadoxine. We examined the prevalence of these mutations in 36 samples from Colombia. Analysed by polymerase chain reaction (PCR) for infection with P. falciparum, 25 (69%) tested positive. These positive isolates were tested further for point mutations in the genes of DHFR (codons 16, 51, 59, 108 and 164) and DHPS (codons 436, 437, 540, 581 and 613) by nested PCR and following mutation‐specific restriction enzyme digestion. Gene mutations occurred in both the DHFR and DHPS gene of the Colombian isolates, suggesting that resistance to antifolate drugs exists or may develop soon in Colombia. |
| doi_str_mv | 10.1046/j.1365-3156.2003.01005.x |
| format | Article |
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Point mutations in the dihydrofolate reductase (DHFR) and dihydropteroate synthase (DHPS) genes of Plasmodium falciparum can lead to an increasing resistance of P. falciparum to pyrimethamine/sulphadoxine. We examined the prevalence of these mutations in 36 samples from Colombia. Analysed by polymerase chain reaction (PCR) for infection with P. falciparum, 25 (69%) tested positive. These positive isolates were tested further for point mutations in the genes of DHFR (codons 16, 51, 59, 108 and 164) and DHPS (codons 436, 437, 540, 581 and 613) by nested PCR and following mutation‐specific restriction enzyme digestion. Gene mutations occurred in both the DHFR and DHPS gene of the Colombian isolates, suggesting that resistance to antifolate drugs exists or may develop soon in Colombia.</description><identifier>ISSN: 1360-2276</identifier><identifier>EISSN: 1365-3156</identifier><identifier>DOI: 10.1046/j.1365-3156.2003.01005.x</identifier><identifier>PMID: 12581437</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Adult ; Aged ; Animals ; Antimalarials - therapeutic use ; Colombia ; dihydrofolate reductase ; dihydropteroate synthase ; Dihydropteroate Synthase - genetics ; Drug Combinations ; drug resistance ; Drug Resistance - genetics ; Genes, Protozoan - genetics ; Humans ; malaria ; Malaria, Falciparum - drug therapy ; Malaria, Falciparum - genetics ; Middle Aged ; Plasmodium falciparum ; Plasmodium falciparum - enzymology ; Plasmodium falciparum - genetics ; Point Mutation - genetics ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Pyrimethamine - therapeutic use ; Sulfadoxine - therapeutic use ; Tetrahydrofolate Dehydrogenase - genetics</subject><ispartof>Tropical medicine & international health, 2003-02, Vol.8 (2), p.129-132</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2805-f882a795a3b35b72615b39f596194ade108c918592a63f3c030e3d278973ff8b3</citedby><cites>FETCH-LOGICAL-c2805-f882a795a3b35b72615b39f596194ade108c918592a63f3c030e3d278973ff8b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-3156.2003.01005.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-3156.2003.01005.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12581437$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schmider, Nadja</creatorcontrib><creatorcontrib>Peyerl‐Hoffmann, Gaby</creatorcontrib><creatorcontrib>Restrepo, Marcos</creatorcontrib><creatorcontrib>Jelinek, Tomas</creatorcontrib><title>Short communication: Point mutations in the dihydrofolate reductase and dihydropteroate synthase genes of Plasmodium falciparum isolates from Colombia</title><title>Tropical medicine & international health</title><addtitle>Trop Med Int Health</addtitle><description>Summary
Point mutations in the dihydrofolate reductase (DHFR) and dihydropteroate synthase (DHPS) genes of Plasmodium falciparum can lead to an increasing resistance of P. falciparum to pyrimethamine/sulphadoxine. We examined the prevalence of these mutations in 36 samples from Colombia. Analysed by polymerase chain reaction (PCR) for infection with P. falciparum, 25 (69%) tested positive. These positive isolates were tested further for point mutations in the genes of DHFR (codons 16, 51, 59, 108 and 164) and DHPS (codons 436, 437, 540, 581 and 613) by nested PCR and following mutation‐specific restriction enzyme digestion. Gene mutations occurred in both the DHFR and DHPS gene of the Colombian isolates, suggesting that resistance to antifolate drugs exists or may develop soon in Colombia.</description><subject>Adult</subject><subject>Aged</subject><subject>Animals</subject><subject>Antimalarials - therapeutic use</subject><subject>Colombia</subject><subject>dihydrofolate reductase</subject><subject>dihydropteroate synthase</subject><subject>Dihydropteroate Synthase - genetics</subject><subject>Drug Combinations</subject><subject>drug resistance</subject><subject>Drug Resistance - genetics</subject><subject>Genes, Protozoan - genetics</subject><subject>Humans</subject><subject>malaria</subject><subject>Malaria, Falciparum - drug therapy</subject><subject>Malaria, Falciparum - genetics</subject><subject>Middle Aged</subject><subject>Plasmodium falciparum</subject><subject>Plasmodium falciparum - enzymology</subject><subject>Plasmodium falciparum - genetics</subject><subject>Point Mutation - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Pyrimethamine - therapeutic use</subject><subject>Sulfadoxine - therapeutic use</subject><subject>Tetrahydrofolate Dehydrogenase - genetics</subject><issn>1360-2276</issn><issn>1365-3156</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0Eoj_wCsir7pJe23HiILFAI6CVWlGJsrYcx2Y8iuOp7YjOi_R5SWam7ZaVj3W-e650D0KYQEmgqi83JWE1LxjhdUkBWAkEgJePb9Dpi_F2r6GgtKlP0FlKGwCoKl6_RyeEckEq1pyip1_rEDPWwftpdFplF8bP-C64MWM_5f0_YTfivDa4d-tdH4MNg8oGR9NPOqtksBr7Z2-bTQyLm3ZjXi_mHzOahIPFd4NKPvRu8tiqQbutirN0aZ-WsI3B41UYgu-c-oDezUwyH4_vOfr9_dv96qq4-fnjevX1ptBUAC-sEFQ1LVesY7xraE14x1rL25q0leoNAaFbInhLVc0s08DAsJ42om2YtaJj5-jikLuN4WEyKUvvkjbDoEYTpiQbBow0lMygOIA6hpSisXIbnVdxJwnIpRO5kcvp5XJ6uXQi953Ix3n003HH1HnTvw4eS5iBLwfgrxvM7r-D5f3t9aLYP6bjne4</recordid><startdate>200302</startdate><enddate>200302</enddate><creator>Schmider, Nadja</creator><creator>Peyerl‐Hoffmann, Gaby</creator><creator>Restrepo, Marcos</creator><creator>Jelinek, Tomas</creator><general>Blackwell Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200302</creationdate><title>Short communication: Point mutations in the dihydrofolate reductase and dihydropteroate synthase genes of Plasmodium falciparum isolates from Colombia</title><author>Schmider, Nadja ; Peyerl‐Hoffmann, Gaby ; Restrepo, Marcos ; Jelinek, Tomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2805-f882a795a3b35b72615b39f596194ade108c918592a63f3c030e3d278973ff8b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Animals</topic><topic>Antimalarials - therapeutic use</topic><topic>Colombia</topic><topic>dihydrofolate reductase</topic><topic>dihydropteroate synthase</topic><topic>Dihydropteroate Synthase - genetics</topic><topic>Drug Combinations</topic><topic>drug resistance</topic><topic>Drug Resistance - genetics</topic><topic>Genes, Protozoan - genetics</topic><topic>Humans</topic><topic>malaria</topic><topic>Malaria, Falciparum - drug therapy</topic><topic>Malaria, Falciparum - genetics</topic><topic>Middle Aged</topic><topic>Plasmodium falciparum</topic><topic>Plasmodium falciparum - enzymology</topic><topic>Plasmodium falciparum - genetics</topic><topic>Point Mutation - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Pyrimethamine - therapeutic use</topic><topic>Sulfadoxine - therapeutic use</topic><topic>Tetrahydrofolate Dehydrogenase - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schmider, Nadja</creatorcontrib><creatorcontrib>Peyerl‐Hoffmann, Gaby</creatorcontrib><creatorcontrib>Restrepo, Marcos</creatorcontrib><creatorcontrib>Jelinek, Tomas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Tropical medicine & international health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schmider, Nadja</au><au>Peyerl‐Hoffmann, Gaby</au><au>Restrepo, Marcos</au><au>Jelinek, Tomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Short communication: Point mutations in the dihydrofolate reductase and dihydropteroate synthase genes of Plasmodium falciparum isolates from Colombia</atitle><jtitle>Tropical medicine & international health</jtitle><addtitle>Trop Med Int Health</addtitle><date>2003-02</date><risdate>2003</risdate><volume>8</volume><issue>2</issue><spage>129</spage><epage>132</epage><pages>129-132</pages><issn>1360-2276</issn><eissn>1365-3156</eissn><abstract>Summary
Point mutations in the dihydrofolate reductase (DHFR) and dihydropteroate synthase (DHPS) genes of Plasmodium falciparum can lead to an increasing resistance of P. falciparum to pyrimethamine/sulphadoxine. We examined the prevalence of these mutations in 36 samples from Colombia. Analysed by polymerase chain reaction (PCR) for infection with P. falciparum, 25 (69%) tested positive. These positive isolates were tested further for point mutations in the genes of DHFR (codons 16, 51, 59, 108 and 164) and DHPS (codons 436, 437, 540, 581 and 613) by nested PCR and following mutation‐specific restriction enzyme digestion. Gene mutations occurred in both the DHFR and DHPS gene of the Colombian isolates, suggesting that resistance to antifolate drugs exists or may develop soon in Colombia.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>12581437</pmid><doi>10.1046/j.1365-3156.2003.01005.x</doi><tpages>4</tpages></addata></record> |
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| source | Wiley Free Content; MEDLINE; IngentaConnect Free/Open Access Journals; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adult Aged Animals Antimalarials - therapeutic use Colombia dihydrofolate reductase dihydropteroate synthase Dihydropteroate Synthase - genetics Drug Combinations drug resistance Drug Resistance - genetics Genes, Protozoan - genetics Humans malaria Malaria, Falciparum - drug therapy Malaria, Falciparum - genetics Middle Aged Plasmodium falciparum Plasmodium falciparum - enzymology Plasmodium falciparum - genetics Point Mutation - genetics Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Pyrimethamine - therapeutic use Sulfadoxine - therapeutic use Tetrahydrofolate Dehydrogenase - genetics |
| title | Short communication: Point mutations in the dihydrofolate reductase and dihydropteroate synthase genes of Plasmodium falciparum isolates from Colombia |
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