Clinical and bacteriological studies of El Tor cholera after ingestion of known inocula in Thai volunteers
Twenty-six healthy adult Thai volunteers were recruited for clinical and bacteriological studies of cholera induced by oral inoculation with Vibrio cholerae El Tor Inaba strain N16961. Vibrio dosages of 0.3 × 10 4, 1.6 × 10 5 and 1.9 × 10 6 c.f.u. were given to three groups of five volunteers, and 2...
Gespeichert in:
| Veröffentlicht in: | Vaccine 1992, Vol.10 (8), p.502-505 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 505 |
|---|---|
| container_issue | 8 |
| container_start_page | 502 |
| container_title | Vaccine |
| container_volume | 10 |
| creator | Suntharasamai, P. Migasena, S. Vongsthongsri, U. Supanaranond, W. Pitisuttitham, P. Supeeranan, L. Chantra, A. Naksrisook, S. |
| description | Twenty-six healthy adult Thai volunteers were recruited for clinical and bacteriological studies of cholera induced by oral inoculation with
Vibrio cholerae El Tor Inaba strain N16961. Vibrio dosages of 0.3 × 10
4, 1.6 × 10
5 and 1.9 × 10
6 c.f.u. were given to three groups of five volunteers, and 2.0 × 10
7 c.f.u. to 11 volunteers. Diarrhoeal attack rates correlated positively with the size of the inocula (
p < 0.01). It was estimated that a diarrhoeal attack rate of 90% (ED
90) would be achievable by inoculation of 1.3 × 10
7 c.f.u. of the organisms. There were no significant differences between the groups in the latent period to positive stool culture, maximum vibrio count per gram of stool and duration of stool positivity. The ED
90 of
V. cholerae obtained may be used as a challenge dose in subsequent studies on protective efficacy of cholera vaccines in Thai adult volunteers. |
| doi_str_mv | 10.1016/0264-410X(92)90347-M |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73029803</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0264410X9290347M</els_id><sourcerecordid>16244492</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-80b2a9e372ae897673930f5e9624aee840d1080f781e9dd844ef1fde6e19bb933</originalsourceid><addsrcrecordid>eNqFkU2LFDEQhoMo67j6DxRyENFDa766O7kIMqwfsIuXEbyFdFLZzZpJ1qR7xX9vemdYb3oqqHqqeHkKoeeUvKWEDu8IG0QnKPn-WrE3inAxdhcP0IbKkXesp_Ih2twjj9GTWq8JIT2n6gSd0IFRQdkGXW9jSMGaiE1yeDJ2hhJyzJd3vTovLkDF2eOziHe5YHuVIxSDjW8gDukS6hxyWokfKf9KrZXtEk2reHdlAr7NcUkzQKlP0SNvYoVnx3qKvn08220_d-dfP33ZfjjvrKDj3EkyMaOAj8yAVOMwcsWJ70ENTBgAKYijRBI_SgrKOSkEeOodDEDVNCnOT9Grw92bkn8uLZ_eh2ohRpMgL1WPnDAlyf_BJkkIoVgDxQG0JddawOubEvam_NaU6PUXehWtV9FaMX33C33R1l4c7y_THtzfpYP8Nn95nJvabPtikg31Hut5r7hcY74_YNCk3QYoutoAyYILBeysXQ7_zvEHP9GmEQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16244492</pqid></control><display><type>article</type><title>Clinical and bacteriological studies of El Tor cholera after ingestion of known inocula in Thai volunteers</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Suntharasamai, P. ; Migasena, S. ; Vongsthongsri, U. ; Supanaranond, W. ; Pitisuttitham, P. ; Supeeranan, L. ; Chantra, A. ; Naksrisook, S.</creator><creatorcontrib>Suntharasamai, P. ; Migasena, S. ; Vongsthongsri, U. ; Supanaranond, W. ; Pitisuttitham, P. ; Supeeranan, L. ; Chantra, A. ; Naksrisook, S.</creatorcontrib><description>Twenty-six healthy adult Thai volunteers were recruited for clinical and bacteriological studies of cholera induced by oral inoculation with
Vibrio cholerae El Tor Inaba strain N16961. Vibrio dosages of 0.3 × 10
4, 1.6 × 10
5 and 1.9 × 10
6 c.f.u. were given to three groups of five volunteers, and 2.0 × 10
7 c.f.u. to 11 volunteers. Diarrhoeal attack rates correlated positively with the size of the inocula (
p < 0.01). It was estimated that a diarrhoeal attack rate of 90% (ED
90) would be achievable by inoculation of 1.3 × 10
7 c.f.u. of the organisms. There were no significant differences between the groups in the latent period to positive stool culture, maximum vibrio count per gram of stool and duration of stool positivity. The ED
90 of
V. cholerae obtained may be used as a challenge dose in subsequent studies on protective efficacy of cholera vaccines in Thai adult volunteers.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/0264-410X(92)90347-M</identifier><identifier>PMID: 1621412</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adult ; Bacterial diseases ; Biological and medical sciences ; Cholera ; Cholera - immunology ; Cholera - microbiology ; Cholera Vaccines - immunology ; ED 90 determination ; Experimental bacterial diseases and models ; experimental infection ; Feces - microbiology ; Humans ; Infectious diseases ; Male ; Medical sciences ; Thai adults ; Vibrio cholerae - isolation & purification</subject><ispartof>Vaccine, 1992, Vol.10 (8), p.502-505</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-80b2a9e372ae897673930f5e9624aee840d1080f781e9dd844ef1fde6e19bb933</citedby><cites>FETCH-LOGICAL-c417t-80b2a9e372ae897673930f5e9624aee840d1080f781e9dd844ef1fde6e19bb933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0264-410X(92)90347-M$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,4022,27921,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5359383$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1621412$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suntharasamai, P.</creatorcontrib><creatorcontrib>Migasena, S.</creatorcontrib><creatorcontrib>Vongsthongsri, U.</creatorcontrib><creatorcontrib>Supanaranond, W.</creatorcontrib><creatorcontrib>Pitisuttitham, P.</creatorcontrib><creatorcontrib>Supeeranan, L.</creatorcontrib><creatorcontrib>Chantra, A.</creatorcontrib><creatorcontrib>Naksrisook, S.</creatorcontrib><title>Clinical and bacteriological studies of El Tor cholera after ingestion of known inocula in Thai volunteers</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Twenty-six healthy adult Thai volunteers were recruited for clinical and bacteriological studies of cholera induced by oral inoculation with
Vibrio cholerae El Tor Inaba strain N16961. Vibrio dosages of 0.3 × 10
4, 1.6 × 10
5 and 1.9 × 10
6 c.f.u. were given to three groups of five volunteers, and 2.0 × 10
7 c.f.u. to 11 volunteers. Diarrhoeal attack rates correlated positively with the size of the inocula (
p < 0.01). It was estimated that a diarrhoeal attack rate of 90% (ED
90) would be achievable by inoculation of 1.3 × 10
7 c.f.u. of the organisms. There were no significant differences between the groups in the latent period to positive stool culture, maximum vibrio count per gram of stool and duration of stool positivity. The ED
90 of
V. cholerae obtained may be used as a challenge dose in subsequent studies on protective efficacy of cholera vaccines in Thai adult volunteers.</description><subject>Adult</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>Cholera</subject><subject>Cholera - immunology</subject><subject>Cholera - microbiology</subject><subject>Cholera Vaccines - immunology</subject><subject>ED 90 determination</subject><subject>Experimental bacterial diseases and models</subject><subject>experimental infection</subject><subject>Feces - microbiology</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Thai adults</subject><subject>Vibrio cholerae - isolation & purification</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo67j6DxRyENFDa766O7kIMqwfsIuXEbyFdFLZzZpJ1qR7xX9vemdYb3oqqHqqeHkKoeeUvKWEDu8IG0QnKPn-WrE3inAxdhcP0IbKkXesp_Ih2twjj9GTWq8JIT2n6gSd0IFRQdkGXW9jSMGaiE1yeDJ2hhJyzJd3vTovLkDF2eOziHe5YHuVIxSDjW8gDukS6hxyWokfKf9KrZXtEk2reHdlAr7NcUkzQKlP0SNvYoVnx3qKvn08220_d-dfP33ZfjjvrKDj3EkyMaOAj8yAVOMwcsWJ70ENTBgAKYijRBI_SgrKOSkEeOodDEDVNCnOT9Grw92bkn8uLZ_eh2ohRpMgL1WPnDAlyf_BJkkIoVgDxQG0JddawOubEvam_NaU6PUXehWtV9FaMX33C33R1l4c7y_THtzfpYP8Nn95nJvabPtikg31Hut5r7hcY74_YNCk3QYoutoAyYILBeysXQ7_zvEHP9GmEQ</recordid><startdate>1992</startdate><enddate>1992</enddate><creator>Suntharasamai, P.</creator><creator>Migasena, S.</creator><creator>Vongsthongsri, U.</creator><creator>Supanaranond, W.</creator><creator>Pitisuttitham, P.</creator><creator>Supeeranan, L.</creator><creator>Chantra, A.</creator><creator>Naksrisook, S.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1992</creationdate><title>Clinical and bacteriological studies of El Tor cholera after ingestion of known inocula in Thai volunteers</title><author>Suntharasamai, P. ; Migasena, S. ; Vongsthongsri, U. ; Supanaranond, W. ; Pitisuttitham, P. ; Supeeranan, L. ; Chantra, A. ; Naksrisook, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-80b2a9e372ae897673930f5e9624aee840d1080f781e9dd844ef1fde6e19bb933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Adult</topic><topic>Bacterial diseases</topic><topic>Biological and medical sciences</topic><topic>Cholera</topic><topic>Cholera - immunology</topic><topic>Cholera - microbiology</topic><topic>Cholera Vaccines - immunology</topic><topic>ED 90 determination</topic><topic>Experimental bacterial diseases and models</topic><topic>experimental infection</topic><topic>Feces - microbiology</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Thai adults</topic><topic>Vibrio cholerae - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suntharasamai, P.</creatorcontrib><creatorcontrib>Migasena, S.</creatorcontrib><creatorcontrib>Vongsthongsri, U.</creatorcontrib><creatorcontrib>Supanaranond, W.</creatorcontrib><creatorcontrib>Pitisuttitham, P.</creatorcontrib><creatorcontrib>Supeeranan, L.</creatorcontrib><creatorcontrib>Chantra, A.</creatorcontrib><creatorcontrib>Naksrisook, S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suntharasamai, P.</au><au>Migasena, S.</au><au>Vongsthongsri, U.</au><au>Supanaranond, W.</au><au>Pitisuttitham, P.</au><au>Supeeranan, L.</au><au>Chantra, A.</au><au>Naksrisook, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinical and bacteriological studies of El Tor cholera after ingestion of known inocula in Thai volunteers</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>1992</date><risdate>1992</risdate><volume>10</volume><issue>8</issue><spage>502</spage><epage>505</epage><pages>502-505</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Twenty-six healthy adult Thai volunteers were recruited for clinical and bacteriological studies of cholera induced by oral inoculation with
Vibrio cholerae El Tor Inaba strain N16961. Vibrio dosages of 0.3 × 10
4, 1.6 × 10
5 and 1.9 × 10
6 c.f.u. were given to three groups of five volunteers, and 2.0 × 10
7 c.f.u. to 11 volunteers. Diarrhoeal attack rates correlated positively with the size of the inocula (
p < 0.01). It was estimated that a diarrhoeal attack rate of 90% (ED
90) would be achievable by inoculation of 1.3 × 10
7 c.f.u. of the organisms. There were no significant differences between the groups in the latent period to positive stool culture, maximum vibrio count per gram of stool and duration of stool positivity. The ED
90 of
V. cholerae obtained may be used as a challenge dose in subsequent studies on protective efficacy of cholera vaccines in Thai adult volunteers.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>1621412</pmid><doi>10.1016/0264-410X(92)90347-M</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-410X |
| ispartof | Vaccine, 1992, Vol.10 (8), p.502-505 |
| issn | 0264-410X 1873-2518 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73029803 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adult Bacterial diseases Biological and medical sciences Cholera Cholera - immunology Cholera - microbiology Cholera Vaccines - immunology ED 90 determination Experimental bacterial diseases and models experimental infection Feces - microbiology Humans Infectious diseases Male Medical sciences Thai adults Vibrio cholerae - isolation & purification |
| title | Clinical and bacteriological studies of El Tor cholera after ingestion of known inocula in Thai volunteers |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T23%3A05%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Clinical%20and%20bacteriological%20studies%20of%20El%20Tor%20cholera%20after%20ingestion%20of%20known%20inocula%20in%20Thai%20volunteers&rft.jtitle=Vaccine&rft.au=Suntharasamai,%20P.&rft.date=1992&rft.volume=10&rft.issue=8&rft.spage=502&rft.epage=505&rft.pages=502-505&rft.issn=0264-410X&rft.eissn=1873-2518&rft.coden=VACCDE&rft_id=info:doi/10.1016/0264-410X(92)90347-M&rft_dat=%3Cproquest_cross%3E16244492%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16244492&rft_id=info:pmid/1621412&rft_els_id=0264410X9290347M&rfr_iscdi=true |