Role of Saccharomyces cerevisiae serine O-acetyltransferase in cysteine biosynthesis

Some strains of Saccharomyces cerevisiae have detectable activities of L-serine O-acetyltransferase (SATase) and O-acetyl-L-serine/O-acetyl-L-homoserine sulfhydrylase (OAS/OAH-SHLase), but synthesize L-cysteine exclusively via cystathionine by cystathionine beta-synthase and cystathionine gamma-lyas...

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Veröffentlicht in:FEMS microbiology letters 2003-01, Vol.218 (2), p.291-297
Hauptverfasser: Takagi, H, Yoshioka, K, Awano, N, Nakamori, S, Ono, B.I
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container_issue 2
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container_title FEMS microbiology letters
container_volume 218
creator Takagi, H
Yoshioka, K
Awano, N
Nakamori, S
Ono, B.I
description Some strains of Saccharomyces cerevisiae have detectable activities of L-serine O-acetyltransferase (SATase) and O-acetyl-L-serine/O-acetyl-L-homoserine sulfhydrylase (OAS/OAH-SHLase), but synthesize L-cysteine exclusively via cystathionine by cystathionine beta-synthase and cystathionine gamma-lyase. To untangle this peculiar feature in sulfur metabolism, we introduced Escherichia coli genes encoding SATase and OAS-SHLase into S. cerevisiae L-cysteine auxotrophs. While the cells expressing SATase grew on medium lacking L-cysteine, those expressing OAS-SHLase did not grow at all. The cells expressing both enzymes grew very well without L-cysteine. These results indicate that S. cerevisiae SATase cannot support L-cysteine biosynthesis and that S. cerevisiae OAS/OAH-SHLase produces L-cysteine if enough OAS is provided by E. coli SATase. It appears as if S. cerevisiae SATase does not possess a metabolic role in vivo either because of very low activity or localization. For example, S. cerevisiae SATase may be localized in the nucleus, thus controlling the level of OAS required for regulation of sulfate assimilation, but playing no role in the direct synthesis of L-cysteine.
doi_str_mv 10.1111/j.1574-6968.2003.tb11531.x
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subjects Acetyltransferase
Acetyltransferases - antagonists & inhibitors
Acetyltransferases - physiology
Auxotrophs
Biological and medical sciences
Biosynthesis
Carbon-Oxygen Lyases - metabolism
cystathionine
cystathionine beta-synthase
cystathionine gamma-lyase
Cysteine
Cysteine - biosynthesis
Cysteine Synthase
E coli
Escherichia coli
Fundamental and applied biological sciences. Psychology
genes
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
L-Serine
Localization
Lyases - metabolism
l‐Cysteine biosynthesis
l‐Serine O‐acetyltransferase
Microbiology
Multienzyme Complexes
Mycology
O‐Acetyl‐l‐serine sulfhydrylase
Saccharomyces cerevisiae
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Serine O-Acetyltransferase
Sulfates
Sulfates - metabolism
Sulfhydrylase
Sulfur
Yeast
title Role of Saccharomyces cerevisiae serine O-acetyltransferase in cysteine biosynthesis
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