Overlapping But Nonidentical Binding Sites on CD2 for CD58 and a Second Ligand CD59
The interaction of the T cell glycoprotein CD2 with one ligand, CD58, contributes to T cell function. We have identified CD59, a glycoprotein with complement-inhibitory function, as a second physiological ligand for CD2. Antibodies to CD59 inhibit CD2-dependent T cell activation in murine T cell hyb...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1992-06, Vol.256 (5065), p.1805-1807 |
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| container_issue | 5065 |
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| container_title | Science (American Association for the Advancement of Science) |
| container_volume | 256 |
| creator | Hahn, William C. Menu, Elisabeth Alfred L. M. Bothwell Sims, Peter J. Bierer, Barbara E. |
| description | The interaction of the T cell glycoprotein CD2 with one ligand, CD58, contributes to T cell function. We have identified CD59, a glycoprotein with complement-inhibitory function, as a second physiological ligand for CD2. Antibodies to CD59 inhibit CD2-dependent T cell activation in murine T cell hybridomas expressing human CD2. In an in vitro binding assay with purified CD58 and CD59, CD2$^+$ cells bind not only immobilized CD58 but also CD59. With two complementary approaches, it was demonstrated that the binding sites on CD2 for CD58 and CD59 are overlapping but nonidentical. These observations suggest that direct interactions between CD2 and both CD58 and CD59 contribute to T cell activation and adhesion. |
| doi_str_mv | 10.1126/science.1377404 |
| format | Article |
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These observations suggest that direct interactions between CD2 and both CD58 and CD59 contribute to T cell activation and adhesion.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1377404</identifier><identifier>PMID: 1377404</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Society for the Advancement of Science</publisher><subject>Amino acids ; Animals ; Antibodies ; Antigen presenting cells ; Antigens, CD - metabolism ; Antigens, Differentiation, T-Lymphocyte - metabolism ; Binding Sites ; Binding sites (Biochemistry) ; Biological and medical sciences ; CD2 Antigens ; CD58 Antigens ; CD59 Antigens ; Cell lines ; Dose-Response Relationship, Drug ; Fundamental and applied biological sciences. 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M. Bothwell</creatorcontrib><creatorcontrib>Sims, Peter J.</creatorcontrib><creatorcontrib>Bierer, Barbara E.</creatorcontrib><title>Overlapping But Nonidentical Binding Sites on CD2 for CD58 and a Second Ligand CD59</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>The interaction of the T cell glycoprotein CD2 with one ligand, CD58, contributes to T cell function. We have identified CD59, a glycoprotein with complement-inhibitory function, as a second physiological ligand for CD2. Antibodies to CD59 inhibit CD2-dependent T cell activation in murine T cell hybridomas expressing human CD2. In an in vitro binding assay with purified CD58 and CD59, CD2$^+$ cells bind not only immobilized CD58 but also CD59. With two complementary approaches, it was demonstrated that the binding sites on CD2 for CD58 and CD59 are overlapping but nonidentical. These observations suggest that direct interactions between CD2 and both CD58 and CD59 contribute to T cell activation and adhesion.</description><subject>Amino acids</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antigen presenting cells</subject><subject>Antigens, CD - metabolism</subject><subject>Antigens, Differentiation, T-Lymphocyte - metabolism</subject><subject>Binding Sites</subject><subject>Binding sites (Biochemistry)</subject><subject>Biological and medical sciences</subject><subject>CD2 Antigens</subject><subject>CD58 Antigens</subject><subject>CD59 Antigens</subject><subject>Cell lines</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Glycoproteins</subject><subject>Humans</subject><subject>Hybridomas</subject><subject>Immunity, Cellular</subject><subject>Immunobiology</subject><subject>In Vitro Techniques</subject><subject>Ligand binding (Biochemistry)</subject><subject>Ligands</subject><subject>Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Mice</subject><subject>Molecules</subject><subject>Receptors, Antigen, T-Cell - physiology</subject><subject>Receptors, Immunologic - metabolism</subject><subject>T cells</subject><subject>T lymphocytes</subject><subject>T-Lymphocytes - immunology</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0s-PEyEUB3BiNGutnr1oMgdjPOzs8mNmgOO2at2k2R6qXieUeUzYTKELM0b_e2k6qWnSxIbDA74f4MBD6C3BN4TQ6jZqC07DDWGcF7h4hiYEyzKXFLPnaIIxq3KBefkSvYrxEeOUSXaFrkY-QevVLwid2u2sa7PZ0GcP3tkGXG-16rKZdc0-WNseYuZdNv9MM-NDqqXIlGsyla1B-zRZ2na_ToF8jV4Y1UV4M9Yp-vH1y_f5t3y5WtzP75a5rjjvc9mAoZJIoYVWzUY0RcEVxrpUBVOGSEkaRYCaclNJTo1pDOFaCW2IUJgrxabo4-HeXfBPA8S-3tqooeuUAz_EmjNMREXFfyGpKGMc0wSvD7BVHdTWGd8HpVtwEFTnHRibtu8IZVKyas_zMzyNBrZWn_OfTnwiPfzuWzXEWN-vHy6mq58X09niUioWyxN6fY5q33XQQp0-cr464bcHroOPMYCpd8FuVfhTE1zvO7UeO7UeWy-deD9-yrDZQvPPH_MPY65i6kUTlNM2HllZMFalt6fo3YE9xt6HY0wF5xXl7C_Nw_R1</recordid><startdate>19920626</startdate><enddate>19920626</enddate><creator>Hahn, William C.</creator><creator>Menu, Elisabeth</creator><creator>Alfred L. 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Bothwell ; Sims, Peter J. ; Bierer, Barbara E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c677t-9def29198c8cadb8d447a00c5a43af1991da1e2f5b6972ffdf17ca8cf18a07aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Amino acids</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antigen presenting cells</topic><topic>Antigens, CD - metabolism</topic><topic>Antigens, Differentiation, T-Lymphocyte - metabolism</topic><topic>Binding Sites</topic><topic>Binding sites (Biochemistry)</topic><topic>Biological and medical sciences</topic><topic>CD2 Antigens</topic><topic>CD58 Antigens</topic><topic>CD59 Antigens</topic><topic>Cell lines</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fundamental and applied biological sciences. 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M. Bothwell</au><au>Sims, Peter J.</au><au>Bierer, Barbara E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overlapping But Nonidentical Binding Sites on CD2 for CD58 and a Second Ligand CD59</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1992-06-26</date><risdate>1992</risdate><volume>256</volume><issue>5065</issue><spage>1805</spage><epage>1807</epage><pages>1805-1807</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>The interaction of the T cell glycoprotein CD2 with one ligand, CD58, contributes to T cell function. We have identified CD59, a glycoprotein with complement-inhibitory function, as a second physiological ligand for CD2. Antibodies to CD59 inhibit CD2-dependent T cell activation in murine T cell hybridomas expressing human CD2. 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| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1992-06, Vol.256 (5065), p.1805-1807 |
| issn | 0036-8075 1095-9203 |
| language | eng |
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| source | MEDLINE; American Association for the Advancement of Science; Jstor Complete Legacy |
| subjects | Amino acids Animals Antibodies Antigen presenting cells Antigens, CD - metabolism Antigens, Differentiation, T-Lymphocyte - metabolism Binding Sites Binding sites (Biochemistry) Biological and medical sciences CD2 Antigens CD58 Antigens CD59 Antigens Cell lines Dose-Response Relationship, Drug Fundamental and applied biological sciences. Psychology Fundamental immunology Glycoproteins Humans Hybridomas Immunity, Cellular Immunobiology In Vitro Techniques Ligand binding (Biochemistry) Ligands Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation Membrane Glycoproteins - metabolism Mice Molecules Receptors, Antigen, T-Cell - physiology Receptors, Immunologic - metabolism T cells T lymphocytes T-Lymphocytes - immunology |
| title | Overlapping But Nonidentical Binding Sites on CD2 for CD58 and a Second Ligand CD59 |
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