A device for automated control of pipette internal pressure for patch-clamp recording

Formation of a high-resistance seal between the tip of a glass recording pipette and the membrane of the recorded cell is the crucial step in patch clamping, or whole cell recording with patch pipettes. Formation of the seal, and subsequent rupture of the membrane for whole cell recording, requires...

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Veröffentlicht in:	Journal of neuroscience methods 2003-02, Vol.123 (1), p.109-115
Hauptverfasser:	Heyward, Philip M, Shipley, Michael T
Format:	Artikel
Sprache:	eng
Schlagworte:	Automated Brain slice Electronics Hydrostatic Hydrostatic Pressure Membrane Potentials - physiology Patch-clamp Patch-Clamp Techniques - instrumentation Pipette Pressure Reproducibility of Results Software
Online-Zugang:	Volltext
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