A human aldehyde dehydrogenase (Aldose reductase) pseudogene: Nucleotide sequence analysis and assignment to chromosome 3
Four cosmid clones containing putative pseudogenes for human aldehyde dehydrogenase (Aldose reductase) were isolated from libraries made to two individuals. These clones show different patterns on digestion with restriction endonucleases and probably represent distinct and separate loci. The DNA seq...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 1992-06, Vol.13 (2), p.465-468 |
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| creator | Brown, Linda Hedge, Philip J. Markham, Alexander F. Graham, Alexander |
| description | Four cosmid clones containing putative pseudogenes for human aldehyde dehydrogenase (Aldose reductase) were isolated from libraries made to two individuals. These clones show different patterns on digestion with restriction endonucleases and probably represent distinct and separate loci. The DNA sequence of one of the putative pseudogenes (cosmid AR.F) was determined, and comparisons demonstrate 89.7% homology with the cDNA sequence of the functional aldose reductase gene. This pseudogene sequence contains no intronic sequences, whereas the functional aldose reductase has nine introns. In addition, the homology disappears in region 5′ to the transcription start site for the cDNA, implying that regulatory elements such as the promoter are missing from this pseudogene. The pseudogene defined by cosmid AR.F has been mapped to chromosome 3 by polymerase chain reaction using amplimers specific for this pseudogene to amplify DNA from somatic cell hybrids. |
| doi_str_mv | 10.1016/0888-7543(92)90274-V |
| format | Article |
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These clones show different patterns on digestion with restriction endonucleases and probably represent distinct and separate loci. The DNA sequence of one of the putative pseudogenes (cosmid AR.F) was determined, and comparisons demonstrate 89.7% homology with the cDNA sequence of the functional aldose reductase gene. This pseudogene sequence contains no intronic sequences, whereas the functional aldose reductase has nine introns. In addition, the homology disappears in region 5′ to the transcription start site for the cDNA, implying that regulatory elements such as the promoter are missing from this pseudogene. The pseudogene defined by cosmid AR.F has been mapped to chromosome 3 by polymerase chain reaction using amplimers specific for this pseudogene to amplify DNA from somatic cell hybrids.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1016/0888-7543(92)90274-V</identifier><identifier>PMID: 1612607</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>aldehyde reductase ; Aldehyde Reductase - genetics ; Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; chromosome 3 ; Chromosomes, Human, Pair 3 ; Cosmids ; Cricetinae ; DNA - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Library ; Genes ; Genes. Genome ; Humans ; Hybrid Cells ; man ; mapping ; Mice ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; nucleotide sequence ; Polymerase Chain Reaction ; Pseudogenes ; Sequence Homology, Nucleic Acid</subject><ispartof>Genomics (San Diego, Calif.), 1992-06, Vol.13 (2), p.465-468</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-5af17bc77e7b2cf1ff203436caf68085a9ae14048ab5a3390027a400407030513</citedby><cites>FETCH-LOGICAL-c417t-5af17bc77e7b2cf1ff203436caf68085a9ae14048ab5a3390027a400407030513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/088875439290274V$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5288490$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1612607$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brown, Linda</creatorcontrib><creatorcontrib>Hedge, Philip J.</creatorcontrib><creatorcontrib>Markham, Alexander F.</creatorcontrib><creatorcontrib>Graham, Alexander</creatorcontrib><title>A human aldehyde dehydrogenase (Aldose reductase) pseudogene: Nucleotide sequence analysis and assignment to chromosome 3</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>Four cosmid clones containing putative pseudogenes for human aldehyde dehydrogenase (Aldose reductase) were isolated from libraries made to two individuals. These clones show different patterns on digestion with restriction endonucleases and probably represent distinct and separate loci. The DNA sequence of one of the putative pseudogenes (cosmid AR.F) was determined, and comparisons demonstrate 89.7% homology with the cDNA sequence of the functional aldose reductase gene. This pseudogene sequence contains no intronic sequences, whereas the functional aldose reductase has nine introns. In addition, the homology disappears in region 5′ to the transcription start site for the cDNA, implying that regulatory elements such as the promoter are missing from this pseudogene. The pseudogene defined by cosmid AR.F has been mapped to chromosome 3 by polymerase chain reaction using amplimers specific for this pseudogene to amplify DNA from somatic cell hybrids.</description><subject>aldehyde reductase</subject><subject>Aldehyde Reductase - genetics</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>chromosome 3</subject><subject>Chromosomes, Human, Pair 3</subject><subject>Cosmids</subject><subject>Cricetinae</subject><subject>DNA - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Library</subject><subject>Genes</subject><subject>Genes. Genome</subject><subject>Humans</subject><subject>Hybrid Cells</subject><subject>man</subject><subject>mapping</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequence</subject><subject>Polymerase Chain Reaction</subject><subject>Pseudogenes</subject><subject>Sequence Homology, Nucleic Acid</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUctu1DAUtRCoTAt_AJIXCLWLwPUjsdMF0qgqD6mCDXRreeybjlESD3aCNH-PMzMqO1gdy-ehq3MIecXgHQPWvAetdaVqKS5bftUCV7K6f0JWDHRb6UY2T8nqUfKcnOf8EwBaofkZOWMN4w2oFdmv6XYe7Eht73G790gPkOIDjjYjvVz3PhZM6Gc3lZ8russ4-4XHa_p1dj3GKRRfxl8zjg6pHW2_zyGXh6c25_AwDjhOdIrUbVMcYo4DUvGCPOtsn_HlCS_Ij4-3328-V3ffPn25Wd9VTjI1VbXtmNo4pVBtuOtY13EQUjTOdo0GXdvWIpMgtd3UVogWShFWAkhQIKBm4oK8PebuUiwX5skMITvseztinLNRAphUtfqvkDVcaX5IlEehSzHnhJ3ZpTDYtDcMzDKNWXo3S--m5eYwjbkvtten_HkzoP9rOm5R-Dcn3mZn-y7Z0YX8KKu51rKFIvtwlGEp7XfAZLILS_E-JHST8TH8-44_KrGq5A</recordid><startdate>19920601</startdate><enddate>19920601</enddate><creator>Brown, Linda</creator><creator>Hedge, Philip J.</creator><creator>Markham, Alexander F.</creator><creator>Graham, Alexander</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T3</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19920601</creationdate><title>A human aldehyde dehydrogenase (Aldose reductase) pseudogene: Nucleotide sequence analysis and assignment to chromosome 3</title><author>Brown, Linda ; Hedge, Philip J. ; Markham, Alexander F. ; Graham, Alexander</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-5af17bc77e7b2cf1ff203436caf68085a9ae14048ab5a3390027a400407030513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>aldehyde reductase</topic><topic>Aldehyde Reductase - genetics</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>chromosome 3</topic><topic>Chromosomes, Human, Pair 3</topic><topic>Cosmids</topic><topic>Cricetinae</topic><topic>DNA - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Library</topic><topic>Genes</topic><topic>Genes. Genome</topic><topic>Humans</topic><topic>Hybrid Cells</topic><topic>man</topic><topic>mapping</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequence</topic><topic>Polymerase Chain Reaction</topic><topic>Pseudogenes</topic><topic>Sequence Homology, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brown, Linda</creatorcontrib><creatorcontrib>Hedge, Philip J.</creatorcontrib><creatorcontrib>Markham, Alexander F.</creatorcontrib><creatorcontrib>Graham, Alexander</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Human Genome Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brown, Linda</au><au>Hedge, Philip J.</au><au>Markham, Alexander F.</au><au>Graham, Alexander</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A human aldehyde dehydrogenase (Aldose reductase) pseudogene: Nucleotide sequence analysis and assignment to chromosome 3</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>1992-06-01</date><risdate>1992</risdate><volume>13</volume><issue>2</issue><spage>465</spage><epage>468</epage><pages>465-468</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>Four cosmid clones containing putative pseudogenes for human aldehyde dehydrogenase (Aldose reductase) were isolated from libraries made to two individuals. These clones show different patterns on digestion with restriction endonucleases and probably represent distinct and separate loci. The DNA sequence of one of the putative pseudogenes (cosmid AR.F) was determined, and comparisons demonstrate 89.7% homology with the cDNA sequence of the functional aldose reductase gene. This pseudogene sequence contains no intronic sequences, whereas the functional aldose reductase has nine introns. In addition, the homology disappears in region 5′ to the transcription start site for the cDNA, implying that regulatory elements such as the promoter are missing from this pseudogene. The pseudogene defined by cosmid AR.F has been mapped to chromosome 3 by polymerase chain reaction using amplimers specific for this pseudogene to amplify DNA from somatic cell hybrids.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1612607</pmid><doi>10.1016/0888-7543(92)90274-V</doi><tpages>4</tpages></addata></record> |
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| subjects | aldehyde reductase Aldehyde Reductase - genetics Amino Acid Sequence Animals Base Sequence Biological and medical sciences chromosome 3 Chromosomes, Human, Pair 3 Cosmids Cricetinae DNA - genetics Fundamental and applied biological sciences. Psychology Gene Library Genes Genes. Genome Humans Hybrid Cells man mapping Mice Molecular and cellular biology Molecular genetics Molecular Sequence Data nucleotide sequence Polymerase Chain Reaction Pseudogenes Sequence Homology, Nucleic Acid |
| title | A human aldehyde dehydrogenase (Aldose reductase) pseudogene: Nucleotide sequence analysis and assignment to chromosome 3 |
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