Screening of Thiol Compounds: Depolarization‐Induced Release of Glutathione and Cysteine from Rat Brain Slices
Superfusates from rat brain slices were screened for thiol compounds after derivatization with monobromo‐bimane by reversed‐phase HPLC. Only glutathione and cysteine were detected. The Ca2+‐dependent release of these compounds from slices of different regions of rat brain was investigated, applying...
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| Veröffentlicht in: | Journal of neurochemistry 1992-07, Vol.59 (1), p.181-189 |
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| container_title | Journal of neurochemistry |
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| creator | Zängerle, Leo Cuénod, Michel Winterhalter, Kaspar H. Do, Kim Quang |
| description | Superfusates from rat brain slices were screened for thiol compounds after derivatization with monobromo‐bimane by reversed‐phase HPLC. Only glutathione and cysteine were detected. The Ca2+‐dependent release of these compounds from slices of different regions of rat brain was investigated, applying a highly sensitive and reproducible quantification method, based on reduction of superfusates with dithiothreitol, reaction of thiols with iodoacetic acid, precolumn derivatization with o‐phthalaldehyde reagent solution, and analysis with reversed‐phase HPLC. This methodology allowed determination of reduced and total thiols in aliquots of the same superfusates. Mostly reduced glutathione and cysteine were released upon K+ depolarization and the Ca2+ dependency suggests that they originate from a neuronal compartment. The GSH release was most prominent in the mesodiencephalon, cortex, hippocampus, and striatum and lowest in the pons‐medulla and cerebellum. This underscores a physiologically significant role for glutathione in CNS neurotransmission. |
| doi_str_mv | 10.1111/j.1471-4159.1992.tb08889.x |
| format | Article |
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Only glutathione and cysteine were detected. The Ca2+‐dependent release of these compounds from slices of different regions of rat brain was investigated, applying a highly sensitive and reproducible quantification method, based on reduction of superfusates with dithiothreitol, reaction of thiols with iodoacetic acid, precolumn derivatization with o‐phthalaldehyde reagent solution, and analysis with reversed‐phase HPLC. This methodology allowed determination of reduced and total thiols in aliquots of the same superfusates. Mostly reduced glutathione and cysteine were released upon K+ depolarization and the Ca2+ dependency suggests that they originate from a neuronal compartment. The GSH release was most prominent in the mesodiencephalon, cortex, hippocampus, and striatum and lowest in the pons‐medulla and cerebellum. This underscores a physiologically significant role for glutathione in CNS neurotransmission.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/j.1471-4159.1992.tb08889.x</identifier><identifier>PMID: 1351924</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Azaserine - pharmacology ; Biochemistry and metabolism ; Biological and medical sciences ; Brain - metabolism ; Central nervous system ; Chromatography, High Pressure Liquid ; Cysteine ; Cysteine - metabolism ; Diazooxonorleucine - pharmacology ; Endogenous transmitter release ; Fundamental and applied biological sciences. Psychology ; gamma-Glutamyltransferase - antagonists & inhibitors ; Glutathione ; Glutathione - metabolism ; Oxidation-Reduction ; Perfusion ; Rat brain slices ; Rats ; Redox ; Sulfhydryl Compounds - metabolism ; Thiols ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of neurochemistry, 1992-07, Vol.59 (1), p.181-189</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4951-c6b34e707614caa515e119ced2387a1fa50f39d8eb5f597a7547706177b78c533</citedby><cites>FETCH-LOGICAL-c4951-c6b34e707614caa515e119ced2387a1fa50f39d8eb5f597a7547706177b78c533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1471-4159.1992.tb08889.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1471-4159.1992.tb08889.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5385338$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1351924$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zängerle, Leo</creatorcontrib><creatorcontrib>Cuénod, Michel</creatorcontrib><creatorcontrib>Winterhalter, Kaspar H.</creatorcontrib><creatorcontrib>Do, Kim Quang</creatorcontrib><title>Screening of Thiol Compounds: Depolarization‐Induced Release of Glutathione and Cysteine from Rat Brain Slices</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>Superfusates from rat brain slices were screened for thiol compounds after derivatization with monobromo‐bimane by reversed‐phase HPLC. Only glutathione and cysteine were detected. The Ca2+‐dependent release of these compounds from slices of different regions of rat brain was investigated, applying a highly sensitive and reproducible quantification method, based on reduction of superfusates with dithiothreitol, reaction of thiols with iodoacetic acid, precolumn derivatization with o‐phthalaldehyde reagent solution, and analysis with reversed‐phase HPLC. This methodology allowed determination of reduced and total thiols in aliquots of the same superfusates. Mostly reduced glutathione and cysteine were released upon K+ depolarization and the Ca2+ dependency suggests that they originate from a neuronal compartment. The GSH release was most prominent in the mesodiencephalon, cortex, hippocampus, and striatum and lowest in the pons‐medulla and cerebellum. This underscores a physiologically significant role for glutathione in CNS neurotransmission.</description><subject>Animals</subject><subject>Azaserine - pharmacology</subject><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Brain - metabolism</subject><subject>Central nervous system</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Cysteine</subject><subject>Cysteine - metabolism</subject><subject>Diazooxonorleucine - pharmacology</subject><subject>Endogenous transmitter release</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gamma-Glutamyltransferase - antagonists & inhibitors</subject><subject>Glutathione</subject><subject>Glutathione - metabolism</subject><subject>Oxidation-Reduction</subject><subject>Perfusion</subject><subject>Rat brain slices</subject><subject>Rats</subject><subject>Redox</subject><subject>Sulfhydryl Compounds - metabolism</subject><subject>Thiols</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVUc1u1DAYtBCobAuPgGQhxC3BX2zHSQ9IkEIpqkBqy9lyHAe8cuzUTkSXE4_AM_IkJNpVOSJ8-WTNzPczg9BzIDks79U2ByYgY8DrHOq6yKeWVFVV53cP0OYeeog2hBRFRgkrHqPjlLaEQMlKOEJHQDnUBdug8VpHY7z1X3Ho8c03GxxuwjCG2XfpFJ-ZMTgV7Q812eB___x14btZmw5fGWdUMqvo3M2TmhalN1j5Dje7NBm7fPoYBnylJvw2KuvxtbPapCfoUa9cMk8P9QR9ef_upvmQXX4-v2jeXGaa1RwyXbaUGUFECUwrxYEbgHqZXNBKKOgVJz2tu8q0vOe1UIIzIUgJQrSi0pzSE_Ry33eM4XY2aZKDTdo4p7wJc5KCksUEXv2TCGXBCGVsIZ7uiTqGlKLp5RjtoOJOApFrLnIrV_Plar5cc5GHXOTdIn52mDK3g-n-SvdBLPiLA66SVq6Pymub7mmcVstN67Kv97Tv1pndfywgP35qoAL6Bz1Zqvw</recordid><startdate>199207</startdate><enddate>199207</enddate><creator>Zängerle, Leo</creator><creator>Cuénod, Michel</creator><creator>Winterhalter, Kaspar H.</creator><creator>Do, Kim Quang</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>199207</creationdate><title>Screening of Thiol Compounds: Depolarization‐Induced Release of Glutathione and Cysteine from Rat Brain Slices</title><author>Zängerle, Leo ; Cuénod, Michel ; Winterhalter, Kaspar H. ; Do, Kim Quang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4951-c6b34e707614caa515e119ced2387a1fa50f39d8eb5f597a7547706177b78c533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Azaserine - pharmacology</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Brain - metabolism</topic><topic>Central nervous system</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Cysteine</topic><topic>Cysteine - metabolism</topic><topic>Diazooxonorleucine - pharmacology</topic><topic>Endogenous transmitter release</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gamma-Glutamyltransferase - antagonists & inhibitors</topic><topic>Glutathione</topic><topic>Glutathione - metabolism</topic><topic>Oxidation-Reduction</topic><topic>Perfusion</topic><topic>Rat brain slices</topic><topic>Rats</topic><topic>Redox</topic><topic>Sulfhydryl Compounds - metabolism</topic><topic>Thiols</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zängerle, Leo</creatorcontrib><creatorcontrib>Cuénod, Michel</creatorcontrib><creatorcontrib>Winterhalter, Kaspar H.</creatorcontrib><creatorcontrib>Do, Kim Quang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zängerle, Leo</au><au>Cuénod, Michel</au><au>Winterhalter, Kaspar H.</au><au>Do, Kim Quang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening of Thiol Compounds: Depolarization‐Induced Release of Glutathione and Cysteine from Rat Brain Slices</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1992-07</date><risdate>1992</risdate><volume>59</volume><issue>1</issue><spage>181</spage><epage>189</epage><pages>181-189</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>Superfusates from rat brain slices were screened for thiol compounds after derivatization with monobromo‐bimane by reversed‐phase HPLC. Only glutathione and cysteine were detected. The Ca2+‐dependent release of these compounds from slices of different regions of rat brain was investigated, applying a highly sensitive and reproducible quantification method, based on reduction of superfusates with dithiothreitol, reaction of thiols with iodoacetic acid, precolumn derivatization with o‐phthalaldehyde reagent solution, and analysis with reversed‐phase HPLC. This methodology allowed determination of reduced and total thiols in aliquots of the same superfusates. Mostly reduced glutathione and cysteine were released upon K+ depolarization and the Ca2+ dependency suggests that they originate from a neuronal compartment. The GSH release was most prominent in the mesodiencephalon, cortex, hippocampus, and striatum and lowest in the pons‐medulla and cerebellum. This underscores a physiologically significant role for glutathione in CNS neurotransmission.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>1351924</pmid><doi>10.1111/j.1471-4159.1992.tb08889.x</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of neurochemistry, 1992-07, Vol.59 (1), p.181-189 |
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| subjects | Animals Azaserine - pharmacology Biochemistry and metabolism Biological and medical sciences Brain - metabolism Central nervous system Chromatography, High Pressure Liquid Cysteine Cysteine - metabolism Diazooxonorleucine - pharmacology Endogenous transmitter release Fundamental and applied biological sciences. Psychology gamma-Glutamyltransferase - antagonists & inhibitors Glutathione Glutathione - metabolism Oxidation-Reduction Perfusion Rat brain slices Rats Redox Sulfhydryl Compounds - metabolism Thiols Vertebrates: nervous system and sense organs |
| title | Screening of Thiol Compounds: Depolarization‐Induced Release of Glutathione and Cysteine from Rat Brain Slices |
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