Functional analysis of biologically distinct genetic variants of simian immunodeficiency virus isolated from a mandrill
We examined the biological properties of two infectious clones of a simian immunodeficiency virus, SIV MND, which were designated as pMD121 and pMD122. Upon transfection into CD4-negative cells, pMD122 generated virions much less efficiently than pMD121. Likewise, the growth kinetics in CD4-positive...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1992-07, Vol.189 (1), p.161-166 |
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| creator | Sakai, Hiroyuki Sakuragi, Jun-Ichi Sakuragi, Sayuri Shibata, Riri Adachi, Akio |
| description | We examined the biological properties of two infectious clones of a simian immunodeficiency virus, SIV
MND, which were designated as pMD121 and pMD122. Upon transfection into CD4-negative cells, pMD122 generated virions much less efficiently than pMD121. Likewise, the growth kinetics in CD4-positive cells of virus derived from pMD122 were remarkably delayed relative to those of virus from pMD121. The cytocidal activity of the MD122 virus was also low. A series of recombinant clones were constructed from pMD121 and pMD122 to determine the sequence responsible for the low virulence of the MD122 virus. The genetic determinant in pMD122 responsible for its properties was mapped to within a region (316 base pairs) encompassing the
tat, rev, and
env coding sequences. Sequence analysis revealed that the two clones differed by only one nucleotide in this region. A nucleotide substitution G (pMD121) to T (pMD122) altered an arginine codon to a serine codon in the first
tat coding exon. Transient transfection experiments showed that the
tat activity of pMD122 was about twofold less than that of pMD121. These findings indicate that small differences in tat activity can have a dramatic effect on the biological behavior of SIV
MND. |
| doi_str_mv | 10.1016/0042-6822(92)90691-H |
| format | Article |
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MND, which were designated as pMD121 and pMD122. Upon transfection into CD4-negative cells, pMD122 generated virions much less efficiently than pMD121. Likewise, the growth kinetics in CD4-positive cells of virus derived from pMD122 were remarkably delayed relative to those of virus from pMD121. The cytocidal activity of the MD122 virus was also low. A series of recombinant clones were constructed from pMD121 and pMD122 to determine the sequence responsible for the low virulence of the MD122 virus. The genetic determinant in pMD122 responsible for its properties was mapped to within a region (316 base pairs) encompassing the
tat, rev, and
env coding sequences. Sequence analysis revealed that the two clones differed by only one nucleotide in this region. A nucleotide substitution G (pMD121) to T (pMD122) altered an arginine codon to a serine codon in the first
tat coding exon. Transient transfection experiments showed that the
tat activity of pMD122 was about twofold less than that of pMD121. These findings indicate that small differences in tat activity can have a dramatic effect on the biological behavior of SIV
MND.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(92)90691-H</identifier><identifier>PMID: 1604807</identifier><identifier>CODEN: VIRLAX</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>AIDS/HIV ; Amino Acid Sequence ; Animals ; Biological and medical sciences ; CD4 Antigens ; Chromosome Mapping ; Cloning, Molecular ; Exons - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, env - genetics ; Genes, rev - genetics ; Genes, tat - genetics ; Genetic Variation ; Genetics ; Microbiology ; Molecular Sequence Data ; Papio - genetics ; Phenotype ; simian immunodeficiency virus ; Simian Immunodeficiency Virus - genetics ; Simian Immunodeficiency Virus - pathogenicity ; Transfection ; Tumor Cells, Cultured ; Virology</subject><ispartof>Virology (New York, N.Y.), 1992-07, Vol.189 (1), p.161-166</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-24fad4253d9e5d6ed7187cd80ab6e3f801c620093e2ad50cb505af25b367c7103</citedby><cites>FETCH-LOGICAL-c332t-24fad4253d9e5d6ed7187cd80ab6e3f801c620093e2ad50cb505af25b367c7103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0042-6822(92)90691-H$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5383700$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1604807$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sakai, Hiroyuki</creatorcontrib><creatorcontrib>Sakuragi, Jun-Ichi</creatorcontrib><creatorcontrib>Sakuragi, Sayuri</creatorcontrib><creatorcontrib>Shibata, Riri</creatorcontrib><creatorcontrib>Adachi, Akio</creatorcontrib><title>Functional analysis of biologically distinct genetic variants of simian immunodeficiency virus isolated from a mandrill</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>We examined the biological properties of two infectious clones of a simian immunodeficiency virus, SIV
MND, which were designated as pMD121 and pMD122. Upon transfection into CD4-negative cells, pMD122 generated virions much less efficiently than pMD121. Likewise, the growth kinetics in CD4-positive cells of virus derived from pMD122 were remarkably delayed relative to those of virus from pMD121. The cytocidal activity of the MD122 virus was also low. A series of recombinant clones were constructed from pMD121 and pMD122 to determine the sequence responsible for the low virulence of the MD122 virus. The genetic determinant in pMD122 responsible for its properties was mapped to within a region (316 base pairs) encompassing the
tat, rev, and
env coding sequences. Sequence analysis revealed that the two clones differed by only one nucleotide in this region. A nucleotide substitution G (pMD121) to T (pMD122) altered an arginine codon to a serine codon in the first
tat coding exon. Transient transfection experiments showed that the
tat activity of pMD122 was about twofold less than that of pMD121. These findings indicate that small differences in tat activity can have a dramatic effect on the biological behavior of SIV
MND.</description><subject>AIDS/HIV</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>CD4 Antigens</subject><subject>Chromosome Mapping</subject><subject>Cloning, Molecular</subject><subject>Exons - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, env - genetics</subject><subject>Genes, rev - genetics</subject><subject>Genes, tat - genetics</subject><subject>Genetic Variation</subject><subject>Genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Papio - genetics</subject><subject>Phenotype</subject><subject>simian immunodeficiency virus</subject><subject>Simian Immunodeficiency Virus - genetics</subject><subject>Simian Immunodeficiency Virus - pathogenicity</subject><subject>Transfection</subject><subject>Tumor Cells, Cultured</subject><subject>Virology</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVGL1TAQhYMo63X1HyjkQUQfqpOkTdoXQRbXKyz4os8hTabLSNqsSXvl_nvbvZf1TSEkE-abk3AOYy8FvBcg9AeAWla6lfJtJ991oDtR7R-xnYBOV6Bq8ZjtHpCn7FkpP2G9GwMX7EJoqFswO_b7epn8TGlykbt1OxYqPA28pxTTLXkX45EHKjOtGL_FCWfy_OAyuWm-JwuNa81pHJcpBRzIE07-yA-Ul8KppOhmDHzIaeSOj24KmWJ8zp4MLhZ8cT4v2Y_rz9-v9tXNty9frz7dVF4pOVeyHlyoZaNCh03QGIxojQ8tuF6jGloQXkuATqF0oQHfN9C4QTa90sYbAeqSvTnp3uX0a8Ey25GKxxjdhGkp1igQ0ojuv6DQUtSt3hTrE-hzKiXjYO8yjS4frQC7BWM31-3muu3WtQVj9-vYq7P-0o8Y_g6dklj7r899V1bXh-wmT-UBa1SrDGyvfzxhuJp2IMy23PuNgTL62YZE__7HHxlHq68</recordid><startdate>199207</startdate><enddate>199207</enddate><creator>Sakai, Hiroyuki</creator><creator>Sakuragi, Jun-Ichi</creator><creator>Sakuragi, Sayuri</creator><creator>Shibata, Riri</creator><creator>Adachi, Akio</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199207</creationdate><title>Functional analysis of biologically distinct genetic variants of simian immunodeficiency virus isolated from a mandrill</title><author>Sakai, Hiroyuki ; Sakuragi, Jun-Ichi ; Sakuragi, Sayuri ; Shibata, Riri ; Adachi, Akio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-24fad4253d9e5d6ed7187cd80ab6e3f801c620093e2ad50cb505af25b367c7103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>AIDS/HIV</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>CD4 Antigens</topic><topic>Chromosome Mapping</topic><topic>Cloning, Molecular</topic><topic>Exons - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, env - genetics</topic><topic>Genes, rev - genetics</topic><topic>Genes, tat - genetics</topic><topic>Genetic Variation</topic><topic>Genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Papio - genetics</topic><topic>Phenotype</topic><topic>simian immunodeficiency virus</topic><topic>Simian Immunodeficiency Virus - genetics</topic><topic>Simian Immunodeficiency Virus - pathogenicity</topic><topic>Transfection</topic><topic>Tumor Cells, Cultured</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sakai, Hiroyuki</creatorcontrib><creatorcontrib>Sakuragi, Jun-Ichi</creatorcontrib><creatorcontrib>Sakuragi, Sayuri</creatorcontrib><creatorcontrib>Shibata, Riri</creatorcontrib><creatorcontrib>Adachi, Akio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sakai, Hiroyuki</au><au>Sakuragi, Jun-Ichi</au><au>Sakuragi, Sayuri</au><au>Shibata, Riri</au><au>Adachi, Akio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional analysis of biologically distinct genetic variants of simian immunodeficiency virus isolated from a mandrill</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1992-07</date><risdate>1992</risdate><volume>189</volume><issue>1</issue><spage>161</spage><epage>166</epage><pages>161-166</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><coden>VIRLAX</coden><abstract>We examined the biological properties of two infectious clones of a simian immunodeficiency virus, SIV
MND, which were designated as pMD121 and pMD122. Upon transfection into CD4-negative cells, pMD122 generated virions much less efficiently than pMD121. Likewise, the growth kinetics in CD4-positive cells of virus derived from pMD122 were remarkably delayed relative to those of virus from pMD121. The cytocidal activity of the MD122 virus was also low. A series of recombinant clones were constructed from pMD121 and pMD122 to determine the sequence responsible for the low virulence of the MD122 virus. The genetic determinant in pMD122 responsible for its properties was mapped to within a region (316 base pairs) encompassing the
tat, rev, and
env coding sequences. Sequence analysis revealed that the two clones differed by only one nucleotide in this region. A nucleotide substitution G (pMD121) to T (pMD122) altered an arginine codon to a serine codon in the first
tat coding exon. Transient transfection experiments showed that the
tat activity of pMD122 was about twofold less than that of pMD121. These findings indicate that small differences in tat activity can have a dramatic effect on the biological behavior of SIV
MND.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1604807</pmid><doi>10.1016/0042-6822(92)90691-H</doi><tpages>6</tpages></addata></record> |
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| ispartof | Virology (New York, N.Y.), 1992-07, Vol.189 (1), p.161-166 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; EZB-FREE-00999 freely available EZB journals |
| subjects | AIDS/HIV Amino Acid Sequence Animals Biological and medical sciences CD4 Antigens Chromosome Mapping Cloning, Molecular Exons - genetics Fundamental and applied biological sciences. Psychology Genes, env - genetics Genes, rev - genetics Genes, tat - genetics Genetic Variation Genetics Microbiology Molecular Sequence Data Papio - genetics Phenotype simian immunodeficiency virus Simian Immunodeficiency Virus - genetics Simian Immunodeficiency Virus - pathogenicity Transfection Tumor Cells, Cultured Virology |
| title | Functional analysis of biologically distinct genetic variants of simian immunodeficiency virus isolated from a mandrill |
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