Antibodies directed against synthetic peptides distinguish between the catalytic subunits of protein phosphatases 1 and 2A
Two antipeptide antibodies (designated type 1 antibody and type 2A antibody) were raised against synthetic peptides, Cys -Thr-Pro-Pro-Arg-Asn-Ser-Ala-Lys-Ala-Lys-Lys and Cys -Val-Thr-Arg-Arg-Thr-Pro-Asp-Try-Phe-Leu, corresponding to the carboxyl termini of the catalytic subunits of protein phosphata...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1992-06, Vol.185 (2), p.657-662 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Wang, Wei Lane, Richard D. Schlender, Keith K. |
| description | Two antipeptide antibodies (designated type 1 antibody and type 2A antibody) were raised against synthetic peptides,
Cys
-Thr-Pro-Pro-Arg-Asn-Ser-Ala-Lys-Ala-Lys-Lys and
Cys
-Val-Thr-Arg-Arg-Thr-Pro-Asp-Try-Phe-Leu, corresponding to the carboxyl termini of the catalytic subunits of protein phosphatase 1 and phosphatase 2A (
Cys
was added for specific coupling to carrier protein). These antipeptide antibodies were highly specific and were useful in discriminating between protein phosphatase 1 and phosphatase 2A in crude extracts or purified preparations. Type 2A antibody reacted with both native and denatured protein phosphatase 2A whereas under the same condition type 1 antibody reacted only with denatured protein phosphatase 1. |
| doi_str_mv | 10.1016/0006-291X(92)91675-G |
| format | Article |
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Cys
-Thr-Pro-Pro-Arg-Asn-Ser-Ala-Lys-Ala-Lys-Lys and
Cys
-Val-Thr-Arg-Arg-Thr-Pro-Asp-Try-Phe-Leu, corresponding to the carboxyl termini of the catalytic subunits of protein phosphatase 1 and phosphatase 2A (
Cys
was added for specific coupling to carrier protein). These antipeptide antibodies were highly specific and were useful in discriminating between protein phosphatase 1 and phosphatase 2A in crude extracts or purified preparations. Type 2A antibody reacted with both native and denatured protein phosphatase 2A whereas under the same condition type 1 antibody reacted only with denatured protein phosphatase 1.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(92)91675-G</identifier><identifier>PMID: 1319144</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Blotting, Western ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; Hydrolases ; Molecular Sequence Data ; Peptides - immunology ; Phosphoprotein Phosphatases - immunology ; Protein Phosphatase 1 ; Protein Phosphatase 2 ; Rabbits</subject><ispartof>Biochemical and biophysical research communications, 1992-06, Vol.185 (2), p.657-662</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c301t-5138be2df8f3f8707b6a0123f12f552a9ee903b74d19746ba9150cb3d06af0f73</citedby><cites>FETCH-LOGICAL-c301t-5138be2df8f3f8707b6a0123f12f552a9ee903b74d19746ba9150cb3d06af0f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(92)91675-G$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5347854$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1319144$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Lane, Richard D.</creatorcontrib><creatorcontrib>Schlender, Keith K.</creatorcontrib><title>Antibodies directed against synthetic peptides distinguish between the catalytic subunits of protein phosphatases 1 and 2A</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Two antipeptide antibodies (designated type 1 antibody and type 2A antibody) were raised against synthetic peptides,
Cys
-Thr-Pro-Pro-Arg-Asn-Ser-Ala-Lys-Ala-Lys-Lys and
Cys
-Val-Thr-Arg-Arg-Thr-Pro-Asp-Try-Phe-Leu, corresponding to the carboxyl termini of the catalytic subunits of protein phosphatase 1 and phosphatase 2A (
Cys
was added for specific coupling to carrier protein). These antipeptide antibodies were highly specific and were useful in discriminating between protein phosphatase 1 and phosphatase 2A in crude extracts or purified preparations. Type 2A antibody reacted with both native and denatured protein phosphatase 2A whereas under the same condition type 1 antibody reacted only with denatured protein phosphatase 1.</description><subject>Amino Acid Sequence</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrolases</subject><subject>Molecular Sequence Data</subject><subject>Peptides - immunology</subject><subject>Phosphoprotein Phosphatases - immunology</subject><subject>Protein Phosphatase 1</subject><subject>Protein Phosphatase 2</subject><subject>Rabbits</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhi0EKtvCG4DkA0JwCHgSJ1lfkFYVLEiVuIDEzXLscdco64SMU7Q8PU6zKjdOc5hv_hl9w9gLEO9AQPNeCNEUpYIfb1T5VkHT1sX-EduAUKIoQcjHbPOAPGWXRD-FAJCNumAXUIECKTfszy6m0A0uIHEXJrQJHTe3JkRKnE4xHTAFy0ccU3D3DKUQb-dAB95h-o0YeWa4Ncn0pwWluZtjSMQHz8dpSBgiHw8DjYeMUI4AbqLj5e4Ze-JNT_j8XK_Y908fv11_Lm6-7r9c724KWwlIRQ3VtsPS-a2v_LYVbdcYAWXlofR1XRqFqETVtdKBamXTGQW1sF3lRGO88G11xV6vufmaXzNS0sdAFvveRBxm0m1ekz3JDMoVtNNANKHX4xSOZjppEHpRrhefevGpVanvlet9Hnt5zp-7I7p_Q6vj3H917huypveTiTbQA1ZXst3WC_ZhxTC7uAs4abIBo8X1LdoN4f93_AWqKp85</recordid><startdate>19920615</startdate><enddate>19920615</enddate><creator>Wang, Wei</creator><creator>Lane, Richard D.</creator><creator>Schlender, Keith K.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920615</creationdate><title>Antibodies directed against synthetic peptides distinguish between the catalytic subunits of protein phosphatases 1 and 2A</title><author>Wang, Wei ; Lane, Richard D. ; Schlender, Keith K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-5138be2df8f3f8707b6a0123f12f552a9ee903b74d19746ba9150cb3d06af0f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Amino Acid Sequence</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrolases</topic><topic>Molecular Sequence Data</topic><topic>Peptides - immunology</topic><topic>Phosphoprotein Phosphatases - immunology</topic><topic>Protein Phosphatase 1</topic><topic>Protein Phosphatase 2</topic><topic>Rabbits</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Lane, Richard D.</creatorcontrib><creatorcontrib>Schlender, Keith K.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Wei</au><au>Lane, Richard D.</au><au>Schlender, Keith K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibodies directed against synthetic peptides distinguish between the catalytic subunits of protein phosphatases 1 and 2A</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1992-06-15</date><risdate>1992</risdate><volume>185</volume><issue>2</issue><spage>657</spage><epage>662</epage><pages>657-662</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Two antipeptide antibodies (designated type 1 antibody and type 2A antibody) were raised against synthetic peptides,
Cys
-Thr-Pro-Pro-Arg-Asn-Ser-Ala-Lys-Ala-Lys-Lys and
Cys
-Val-Thr-Arg-Arg-Thr-Pro-Asp-Try-Phe-Leu, corresponding to the carboxyl termini of the catalytic subunits of protein phosphatase 1 and phosphatase 2A (
Cys
was added for specific coupling to carrier protein). These antipeptide antibodies were highly specific and were useful in discriminating between protein phosphatase 1 and phosphatase 2A in crude extracts or purified preparations. Type 2A antibody reacted with both native and denatured protein phosphatase 2A whereas under the same condition type 1 antibody reacted only with denatured protein phosphatase 1.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1319144</pmid><doi>10.1016/0006-291X(92)91675-G</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1992-06, Vol.185 (2), p.657-662 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acid Sequence Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Blotting, Western Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Hydrolases Molecular Sequence Data Peptides - immunology Phosphoprotein Phosphatases - immunology Protein Phosphatase 1 Protein Phosphatase 2 Rabbits |
| title | Antibodies directed against synthetic peptides distinguish between the catalytic subunits of protein phosphatases 1 and 2A |
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