Zinc Transporters 1, 2 and 4 Are Differentially Expressed and Localized in Rats during Pregnancy and Lactation
Zinc metabolism is controlled within relatively restricted limits throughout the life cycle. Expression and localization of zinc transporters 1, 2 and 4 during pregnancy and lactation in small intestine, mammary gland and liver of the rat were investigated using Northern analysis, Western blotting a...
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| Veröffentlicht in: | The Journal of nutrition 2003-02, Vol.133 (2), p.342-351 |
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| creator | Liuzzi, Juan P. Bobo, Jeffrey A. Cui, Li McMahon, Robert J. Cousins, Robert J. |
| description | Zinc metabolism is controlled within relatively restricted limits throughout the life cycle. Expression and localization of zinc transporters 1, 2 and 4 during pregnancy and lactation in small intestine, mammary gland and liver of the rat were investigated using Northern analysis, Western blotting and immunohistochemistry. In maternal tissues, zinc transporter 4 was the most widely expressed among these zinc transporters in the tissues examined. In small intestine and liver, zinc transporter 4 increased from levels found during late gestation, but zinc transporter 1 did not. Zinc transporter 2 expression in small intestine was transient, being highest around parturition, and was not detected in liver. Immunohistochemistry revealed unique patterns of zinc transporter localization at different stages of development. In the placenta, zinc transporters 1 and 4 were found concentrated along the villous visceral splanchnopleure. In the mammary gland, zinc transporter 4 was most abundant in cells surrounding the alveolar ducts and oriented to the basement lamina. All three transporters were highly expressed in neonatal small intestine, principally near the apical surface, but zinc transporters 1 and 4 increased in abundance at the basolateral surface during development. Zinc transporter 2 was oriented apically, directly adjacent to the microvilli of enterocytes. Within the intestine, expression of each transporter was limited to enterocytes. These results support a role for these transporters in maintaining an adequate zinc supply derived from the maternal diet for zinc acquisition and use by the fetus and neonate. |
| doi_str_mv | 10.1093/jn/133.2.342 |
| format | Article |
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Expression and localization of zinc transporters 1, 2 and 4 during pregnancy and lactation in small intestine, mammary gland and liver of the rat were investigated using Northern analysis, Western blotting and immunohistochemistry. In maternal tissues, zinc transporter 4 was the most widely expressed among these zinc transporters in the tissues examined. In small intestine and liver, zinc transporter 4 increased from levels found during late gestation, but zinc transporter 1 did not. Zinc transporter 2 expression in small intestine was transient, being highest around parturition, and was not detected in liver. Immunohistochemistry revealed unique patterns of zinc transporter localization at different stages of development. In the placenta, zinc transporters 1 and 4 were found concentrated along the villous visceral splanchnopleure. In the mammary gland, zinc transporter 4 was most abundant in cells surrounding the alveolar ducts and oriented to the basement lamina. All three transporters were highly expressed in neonatal small intestine, principally near the apical surface, but zinc transporters 1 and 4 increased in abundance at the basolateral surface during development. Zinc transporter 2 was oriented apically, directly adjacent to the microvilli of enterocytes. Within the intestine, expression of each transporter was limited to enterocytes. These results support a role for these transporters in maintaining an adequate zinc supply derived from the maternal diet for zinc acquisition and use by the fetus and neonate.</description><identifier>ISSN: 0022-3166</identifier><identifier>EISSN: 1541-6100</identifier><identifier>DOI: 10.1093/jn/133.2.342</identifier><identifier>PMID: 12566465</identifier><identifier>CODEN: JONUAI</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>Analysis of Variance ; Animal reproduction ; Animals ; Biological and medical sciences ; Blotting, Northern ; Blotting, Western ; Breast - metabolism ; Breast - ultrastructure ; Carrier Proteins - genetics ; Carrier Proteins - physiology ; Embryonic and Fetal Development ; enterocytes ; Female ; fetal development ; fetus ; Fundamental and applied biological sciences. Psychology ; gene expression ; gene expression regulation ; immunohistochemistry ; Intestine, Small - metabolism ; Intestine, Small - ultrastructure ; lactation ; Lactation - metabolism ; liver ; Liver - metabolism ; Liver - ultrastructure ; mammary glands ; maternal nutrition ; Metabolism ; microvilli ; Mother. Fetoplacental unit. Mammary gland. Milk ; neonates ; parturition ; placenta ; Placenta - metabolism ; Placenta - ultrastructure ; Pregnancy ; Pregnancy. Parturition. Lactation ; Rats ; Rodents ; small intestine ; transport ; transporters ; Vertebrates: reproduction ; Western blotting ; Zinc ; Zinc - metabolism</subject><ispartof>The Journal of nutrition, 2003-02, Vol.133 (2), p.342-351</ispartof><rights>2003 American Society for Nutrition.</rights><rights>2003 INIST-CNRS</rights><rights>Copyright American Institute of Nutrition Feb 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c519t-d6120fd8ef95bfbd39527268a71b4d628d15cf4735298cac97719ede50ba865e3</citedby><cites>FETCH-LOGICAL-c519t-d6120fd8ef95bfbd39527268a71b4d628d15cf4735298cac97719ede50ba865e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14530360$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12566465$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liuzzi, Juan P.</creatorcontrib><creatorcontrib>Bobo, Jeffrey A.</creatorcontrib><creatorcontrib>Cui, Li</creatorcontrib><creatorcontrib>McMahon, Robert J.</creatorcontrib><creatorcontrib>Cousins, Robert J.</creatorcontrib><title>Zinc Transporters 1, 2 and 4 Are Differentially Expressed and Localized in Rats during Pregnancy and Lactation</title><title>The Journal of nutrition</title><addtitle>J Nutr</addtitle><description>Zinc metabolism is controlled within relatively restricted limits throughout the life cycle. Expression and localization of zinc transporters 1, 2 and 4 during pregnancy and lactation in small intestine, mammary gland and liver of the rat were investigated using Northern analysis, Western blotting and immunohistochemistry. In maternal tissues, zinc transporter 4 was the most widely expressed among these zinc transporters in the tissues examined. In small intestine and liver, zinc transporter 4 increased from levels found during late gestation, but zinc transporter 1 did not. Zinc transporter 2 expression in small intestine was transient, being highest around parturition, and was not detected in liver. Immunohistochemistry revealed unique patterns of zinc transporter localization at different stages of development. In the placenta, zinc transporters 1 and 4 were found concentrated along the villous visceral splanchnopleure. In the mammary gland, zinc transporter 4 was most abundant in cells surrounding the alveolar ducts and oriented to the basement lamina. All three transporters were highly expressed in neonatal small intestine, principally near the apical surface, but zinc transporters 1 and 4 increased in abundance at the basolateral surface during development. Zinc transporter 2 was oriented apically, directly adjacent to the microvilli of enterocytes. Within the intestine, expression of each transporter was limited to enterocytes. These results support a role for these transporters in maintaining an adequate zinc supply derived from the maternal diet for zinc acquisition and use by the fetus and neonate.</description><subject>Analysis of Variance</subject><subject>Animal reproduction</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Blotting, Western</subject><subject>Breast - metabolism</subject><subject>Breast - ultrastructure</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - physiology</subject><subject>Embryonic and Fetal Development</subject><subject>enterocytes</subject><subject>Female</subject><subject>fetal development</subject><subject>fetus</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>gene expression regulation</subject><subject>immunohistochemistry</subject><subject>Intestine, Small - metabolism</subject><subject>Intestine, Small - ultrastructure</subject><subject>lactation</subject><subject>Lactation - metabolism</subject><subject>liver</subject><subject>Liver - metabolism</subject><subject>Liver - ultrastructure</subject><subject>mammary glands</subject><subject>maternal nutrition</subject><subject>Metabolism</subject><subject>microvilli</subject><subject>Mother. Fetoplacental unit. Mammary gland. Milk</subject><subject>neonates</subject><subject>parturition</subject><subject>placenta</subject><subject>Placenta - metabolism</subject><subject>Placenta - ultrastructure</subject><subject>Pregnancy</subject><subject>Pregnancy. Parturition. Lactation</subject><subject>Rats</subject><subject>Rodents</subject><subject>small intestine</subject><subject>transport</subject><subject>transporters</subject><subject>Vertebrates: reproduction</subject><subject>Western blotting</subject><subject>Zinc</subject><subject>Zinc - metabolism</subject><issn>0022-3166</issn><issn>1541-6100</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0c1rFDEYBvAgil2rN88aBD11tvmeybHU-gELirYXLyGbvFmyzGa2yYy4_vVmnYWCeAqBHy8Pz4PQS0qWlGh-uU2XlPMlW3LBHqEFlYI2ihLyGC0IYazhVKkz9KyULSGECt09RWeUSaWEkguUfsTk8G22qeyHPEIumF5ghm3yWOCrDPh9DAEypDHavj_gm1_7DKWA_0tWg7N9_F1_MeFvdizYTzmmDf6aYZNscoeZWTfaMQ7pOXoSbF_gxek9R3cfbm6vPzWrLx8_X1-tGiepHhuvKCPBdxC0XIe151qylqnOtnQtvGKdp9IF0XLJdOes021LNXiQZG07JYGfo3fz3X0e7icoo9nF4qDvbYJhKqblhMjaR4Vv_oHbYcqpZjNUt0Io1eqKLmbk8lBKhmD2Oe5sPhhKzHEEs02mjmCYqSNU_up0c1rvwD_gU-sVvD0BW2p_obbvYnlwQnLC1THc69kFOxi7ydXcfWd1xOOQopVtFWoWUMv8GSGb4iIkBz5mcKPxQ_x_xj_x9qk1</recordid><startdate>20030201</startdate><enddate>20030201</enddate><creator>Liuzzi, Juan P.</creator><creator>Bobo, Jeffrey A.</creator><creator>Cui, Li</creator><creator>McMahon, Robert J.</creator><creator>Cousins, Robert J.</creator><general>Elsevier Inc</general><general>American Institute of Nutrition</general><general>American Society for Nutritional Sciences</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>20030201</creationdate><title>Zinc Transporters 1, 2 and 4 Are Differentially Expressed and Localized in Rats during Pregnancy and Lactation</title><author>Liuzzi, Juan P. ; Bobo, Jeffrey A. ; Cui, Li ; McMahon, Robert J. ; Cousins, Robert J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c519t-d6120fd8ef95bfbd39527268a71b4d628d15cf4735298cac97719ede50ba865e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Analysis of Variance</topic><topic>Animal reproduction</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Blotting, Western</topic><topic>Breast - metabolism</topic><topic>Breast - ultrastructure</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - physiology</topic><topic>Embryonic and Fetal Development</topic><topic>enterocytes</topic><topic>Female</topic><topic>fetal development</topic><topic>fetus</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>gene expression regulation</topic><topic>immunohistochemistry</topic><topic>Intestine, Small - metabolism</topic><topic>Intestine, Small - ultrastructure</topic><topic>lactation</topic><topic>Lactation - metabolism</topic><topic>liver</topic><topic>Liver - metabolism</topic><topic>Liver - ultrastructure</topic><topic>mammary glands</topic><topic>maternal nutrition</topic><topic>Metabolism</topic><topic>microvilli</topic><topic>Mother. Fetoplacental unit. Mammary gland. Milk</topic><topic>neonates</topic><topic>parturition</topic><topic>placenta</topic><topic>Placenta - metabolism</topic><topic>Placenta - ultrastructure</topic><topic>Pregnancy</topic><topic>Pregnancy. Parturition. Lactation</topic><topic>Rats</topic><topic>Rodents</topic><topic>small intestine</topic><topic>transport</topic><topic>transporters</topic><topic>Vertebrates: reproduction</topic><topic>Western blotting</topic><topic>Zinc</topic><topic>Zinc - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liuzzi, Juan P.</creatorcontrib><creatorcontrib>Bobo, Jeffrey A.</creatorcontrib><creatorcontrib>Cui, Li</creatorcontrib><creatorcontrib>McMahon, Robert J.</creatorcontrib><creatorcontrib>Cousins, Robert J.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liuzzi, Juan P.</au><au>Bobo, Jeffrey A.</au><au>Cui, Li</au><au>McMahon, Robert J.</au><au>Cousins, Robert J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Zinc Transporters 1, 2 and 4 Are Differentially Expressed and Localized in Rats during Pregnancy and Lactation</atitle><jtitle>The Journal of nutrition</jtitle><addtitle>J Nutr</addtitle><date>2003-02-01</date><risdate>2003</risdate><volume>133</volume><issue>2</issue><spage>342</spage><epage>351</epage><pages>342-351</pages><issn>0022-3166</issn><eissn>1541-6100</eissn><coden>JONUAI</coden><abstract>Zinc metabolism is controlled within relatively restricted limits throughout the life cycle. Expression and localization of zinc transporters 1, 2 and 4 during pregnancy and lactation in small intestine, mammary gland and liver of the rat were investigated using Northern analysis, Western blotting and immunohistochemistry. In maternal tissues, zinc transporter 4 was the most widely expressed among these zinc transporters in the tissues examined. In small intestine and liver, zinc transporter 4 increased from levels found during late gestation, but zinc transporter 1 did not. Zinc transporter 2 expression in small intestine was transient, being highest around parturition, and was not detected in liver. Immunohistochemistry revealed unique patterns of zinc transporter localization at different stages of development. In the placenta, zinc transporters 1 and 4 were found concentrated along the villous visceral splanchnopleure. In the mammary gland, zinc transporter 4 was most abundant in cells surrounding the alveolar ducts and oriented to the basement lamina. All three transporters were highly expressed in neonatal small intestine, principally near the apical surface, but zinc transporters 1 and 4 increased in abundance at the basolateral surface during development. Zinc transporter 2 was oriented apically, directly adjacent to the microvilli of enterocytes. Within the intestine, expression of each transporter was limited to enterocytes. These results support a role for these transporters in maintaining an adequate zinc supply derived from the maternal diet for zinc acquisition and use by the fetus and neonate.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>12566465</pmid><doi>10.1093/jn/133.2.342</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis of Variance Animal reproduction Animals Biological and medical sciences Blotting, Northern Blotting, Western Breast - metabolism Breast - ultrastructure Carrier Proteins - genetics Carrier Proteins - physiology Embryonic and Fetal Development enterocytes Female fetal development fetus Fundamental and applied biological sciences. Psychology gene expression gene expression regulation immunohistochemistry Intestine, Small - metabolism Intestine, Small - ultrastructure lactation Lactation - metabolism liver Liver - metabolism Liver - ultrastructure mammary glands maternal nutrition Metabolism microvilli Mother. Fetoplacental unit. Mammary gland. Milk neonates parturition placenta Placenta - metabolism Placenta - ultrastructure Pregnancy Pregnancy. Parturition. Lactation Rats Rodents small intestine transport transporters Vertebrates: reproduction Western blotting Zinc Zinc - metabolism |
| title | Zinc Transporters 1, 2 and 4 Are Differentially Expressed and Localized in Rats during Pregnancy and Lactation |
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